PGA31_CANAL
ID PGA31_CANAL Reviewed; 293 AA.
AC Q5A5U9; A0A1D8PM04; Q5A631;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 12-APR-2017, sequence version 2.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=Cell wall protein PGA31;
DE AltName: Full=GPI-anchored protein 31;
DE AltName: Full=LDG family protein 5;
DE Flags: Precursor;
GN Name=PGA31; Synonyms=LDG5; OrderedLocusNames=CAALFM_C404080CA;
GN ORFNames=CaO19.12761, CaO19.5302;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP INDUCTION.
RX PubMed=12397174; DOI=10.1073/pnas.232566499;
RA Lan C.Y., Newport G., Murillo L.A., Jones T., Scherer S., Davis R.W.,
RA Agabian N.;
RT "Metabolic specialization associated with phenotypic switching in
RT Candidaalbicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:14907-14912(2002).
RN [5]
RP PREDICTION OF GPI-ANCHOR.
RX PubMed=12845604; DOI=10.1002/yea.1007;
RA De Groot P.W., Hellingwerf K.J., Klis F.M.;
RT "Genome-wide identification of fungal GPI proteins.";
RL Yeast 20:781-796(2003).
RN [6]
RP INDUCTION.
RX PubMed=16455273; DOI=10.1016/j.fgb.2005.12.002;
RA Castillo L., Martinez A.I., Garcera A., Garcia-Martinez J.,
RA Ruiz-Herrera J., Valentin E., Sentandreu R.;
RT "Genomic response programs of Candida albicans following protoplasting and
RT regeneration.";
RL Fungal Genet. Biol. 43:124-134(2006).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=18765290; DOI=10.1016/j.fgb.2008.08.003;
RA Plaine A., Walker L., Da Costa G., Mora-Montes H.M., McKinnon A., Gow N.A.,
RA Gaillardin C., Munro C.A., Richard M.L.;
RT "Functional analysis of Candida albicans GPI-anchored proteins: roles in
RT cell wall integrity and caspofungin sensitivity.";
RL Fungal Genet. Biol. 45:1404-1414(2008).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=18712765; DOI=10.1002/pmic.200800110;
RA Castillo L., Calvo E., Martinez A.I., Ruiz-Herrera J., Valentin E.,
RA Lopez J.A., Sentandreu R.;
RT "A study of the Candida albicans cell wall proteome.";
RL Proteomics 8:3871-3881(2008).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=22997008; DOI=10.1002/pmic.201200228;
RA Ene I.V., Heilmann C.J., Sorgo A.G., Walker L.A., de Koster C.G.,
RA Munro C.A., Klis F.M., Brown A.J.;
RT "Carbon source-induced reprogramming of the cell wall proteome and
RT secretome modulates the adherence and drug resistance of the fungal
RT pathogen Candida albicans.";
RL Proteomics 12:3164-3179(2012).
RN [10]
RP INDUCTION.
RX PubMed=23208712; DOI=10.1128/aac.02040-12;
RA Silva L.V., Sanguinetti M., Vandeputte P., Torelli R., Rochat B.,
RA Sanglard D.;
RT "Milbemycins: more than efflux inhibitors for fungal pathogens.";
RL Antimicrob. Agents Chemother. 57:873-886(2013).
RN [11]
RP INDUCTION.
RX PubMed=23563485; DOI=10.1128/ec.00071-13;
RA Srikantha T., Daniels K.J., Pujol C., Kim E., Soll D.R.;
RT "Identification of genes upregulated by the transcription factor Bcr1 that
RT are involved in impermeability, impenetrability, and drug resistance of
RT Candida albicans a/alpha biofilms.";
RL Eukaryot. Cell 12:875-888(2013).
CC -!- FUNCTION: Component of the cell wall involved in virulence which plays
CC a role in the relationship between C.albicans and the host (By
CC similarity). Involved in the regulation or assembly of chitin within
CC the cell wall. {ECO:0000250, ECO:0000269|PubMed:18765290}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall. Membrane; Lipid-anchor, GPI-
CC anchor.
CC -!- INDUCTION: Expression is regulated upon white-opaque switch, during
CC cell wall regeneration, and when cells are grown on lactate. Also up-
CC regulated upon milbemycin A3 oxim derivative (A3Ox) treatment.
CC Repressed by BCR1. {ECO:0000269|PubMed:12397174,
CC ECO:0000269|PubMed:16455273, ECO:0000269|PubMed:22997008,
CC ECO:0000269|PubMed:23208712, ECO:0000269|PubMed:23563485}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC -!- DISRUPTION PHENOTYPE: Leads to hypersensitivity to caspofungin.
CC {ECO:0000269|PubMed:18765290}.
CC -!- SIMILARITY: Belongs to the SRP1/TIP1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AOW29157.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CP017626; AOW29157.1; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_717105.1; XM_712012.1.
DR AlphaFoldDB; Q5A5U9; -.
DR GeneID; 3641226; -.
DR KEGG; cal:CAALFM_C404080CA; -.
DR CGD; CAL0000183944; PGA31.
DR eggNOG; ENOG502SXWZ; Eukaryota.
DR HOGENOM; CLU_083354_0_0_1; -.
DR OrthoDB; 1611342at2759; -.
DR PRO; PR:Q5A5U9; -.
DR Proteomes; UP000000559; Chromosome 4.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
PE 1: Evidence at protein level;
KW Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Secreted; Signal; Virulence.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..271
FT /note="Cell wall protein PGA31"
FT /id="PRO_0000424729"
FT PROPEP 272..293
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000424730"
FT REGION 161..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 233..262
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 233..254
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 271
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
FT CARBOHYD 131
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 190
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 293 AA; 29735 MW; F8FD74DCBD1C544A CRC64;
MKFLTAASLL TLSSSALAAI KDIQLYAQSS NNEVNDFGIS SRHEGAALNY LFLAAPGVAE
NLKYDDETKT VYTELKAGSS TVRQPLNVGN TVLQLGGSGD GTKVDIAEDG TLSFDGSDSV
GAAKNINDPY NYSKDSYAVV KGGDGAIPIK LVAKFTGDDK ESASSSSSSA APEPTASSSE
APKETPVYSN STVTLYTTYC PLSTTITLTV CSDVCTPTVI ETSGSVTVSS VQVPSKTASS
EAAPPKTTVD SVSKPAPSGK KPTAAVTSFE GAANALTGGS VAIAVAAAIG LVF
