PGA47_CANAL
ID PGA47_CANAL Reviewed; 653 AA.
AC G1UBC2; A0A1D8PID9; G1UB13;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 2.
DT 25-MAY-2022, entry version 49.
DE RecName: Full=Cell wall adhesin EAP1;
DE AltName: Full=Enhanced adherence to polystyrene protein 1;
DE AltName: Full=GPI-anchored protein 47;
DE Flags: Precursor;
GN Name=EAP1; Synonyms=PGA47; OrderedLocusNames=CAALFM_C209530WA;
GN ORFNames=CaO19.1401, CaO19.8979;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP FUNCTION, AND INDUCTION.
RX PubMed=14665461; DOI=10.1128/ec.2.6.1266-1273.2003;
RA Li F., Palecek S.P.;
RT "EAP1, a Candida albicans gene involved in binding human epithelial
RT cells.";
RL Eukaryot. Cell 2:1266-1273(2003).
RN [5]
RP PREDICTION OF GPI-ANCHOR.
RX PubMed=12845604; DOI=10.1002/yea.1007;
RA De Groot P.W., Hellingwerf K.J., Klis F.M.;
RT "Genome-wide identification of fungal GPI proteins.";
RL Yeast 20:781-796(2003).
RN [6]
RP INDUCTION.
RX PubMed=15387822; DOI=10.1111/j.1365-2958.2004.04214.x;
RA Lan C.Y., Rodarte G., Murillo L.A., Jones T., Davis R.W., Dungan J.,
RA Newport G., Agabian N.;
RT "Regulatory networks affected by iron availability in Candida albicans.";
RL Mol. Microbiol. 53:1451-1469(2004).
RN [7]
RP FUNCTION.
RX PubMed=16321041; DOI=10.1021/bp050236c;
RA Li F., Palecek S.P.;
RT "Identification of Candida albicans genes that induce Saccharomyces
RT cerevisiae cell adhesion and morphogenesis.";
RL Biotechnol. Prog. 21:1601-1609(2005).
RN [8]
RP SUBCELLULAR LOCATION, GPI-ANCHOR, FUNCTION, AND INDUCTION.
RX PubMed=17416898; DOI=10.1128/ec.00049-07;
RA Li F., Svarovsky M.J., Karlsson A.J., Wagner J.P., Marchillo K., Oshel P.,
RA Andes D., Palecek S.P.;
RT "Eap1p, an adhesin that mediates Candida albicans biofilm formation in
RT vitro and in vivo.";
RL Eukaryot. Cell 6:931-939(2007).
RN [9]
RP SUBCELLULAR LOCATION, FUNCTION, AND DOMAIN.
RX PubMed=18375812; DOI=10.1099/mic.0.2007/013789-0;
RA Li F., Palecek S.P.;
RT "Distinct domains of the Candida albicans adhesin Eap1p mediate cell-cell
RT and cell-substrate interactions.";
RL Microbiology 154:1193-1203(2008).
RN [10]
RP INDUCTION.
RX PubMed=19798425; DOI=10.1371/journal.ppat.1000601;
RA Sahni N., Yi S., Daniels K.J., Srikantha T., Pujol C., Soll D.R.;
RT "Genes selectively up-regulated by pheromone in white cells are involved in
RT biofilm formation in Candida albicans.";
RL PLoS Pathog. 5:E1000601-E1000601(2009).
RN [11]
RP FUNCTION.
RX PubMed=20709785; DOI=10.1128/ec.00103-10;
RA Nobbs A.H., Vickerman M.M., Jenkinson H.F.;
RT "Heterologous expression of Candida albicans cell wall-associated adhesins
RT in Saccharomyces cerevisiae Reveals differential specificities in adherence
RT and biofilm formation and in binding oral Streptococcus gordonii.";
RL Eukaryot. Cell 9:1622-1634(2010).
RN [12]
RP INDUCTION.
RX PubMed=21566087; DOI=10.1099/jmm.0.032037-0;
RA Kucharikova S., Tournu H., Lagrou K., Van Dijck P., Bujdakova H.;
RT "Detailed comparison of Candida albicans and Candida glabrata biofilms
RT under different conditions and their susceptibility to caspofungin and
RT anidulafungin.";
RL J. Med. Microbiol. 60:1261-1269(2011).
RN [13]
RP INDUCTION.
RX PubMed=23563485; DOI=10.1128/ec.00071-13;
RA Srikantha T., Daniels K.J., Pujol C., Kim E., Soll D.R.;
RT "Identification of genes upregulated by the transcription factor Bcr1 that
RT are involved in impermeability, impenetrability, and drug resistance of
RT Candida albicans a/alpha biofilms.";
RL Eukaryot. Cell 12:875-888(2013).
RN [14]
RP INDUCTION.
RX PubMed=23948468; DOI=10.1016/j.micpath.2013.07.003;
RA Tian J., Weng L.X., Zhang Y.Q., Wang L.H.;
RT "BDSF inhibits Candida albicans adherence to urinary catheters.";
RL Microb. Pathog. 64:33-38(2013).
RN [15]
RP INDUCTION.
RX PubMed=23704884; DOI=10.1371/journal.pone.0062902;
RA Samaranayake Y.H., Cheung B.P., Yau J.Y., Yeung S.K., Samaranayake L.P.;
RT "Human serum promotes Candida albicans biofilm growth and virulence gene
RT expression on silicone biomaterial.";
RL PLoS ONE 8:E62902-E62902(2013).
CC -!- FUNCTION: Cell wall protein which mediates cell-cell and cell-substrate
CC adhesion. Required for biofilm formation and plays a role in virulence.
CC {ECO:0000269|PubMed:14665461, ECO:0000269|PubMed:16321041,
CC ECO:0000269|PubMed:17416898, ECO:0000269|PubMed:18375812,
CC ECO:0000269|PubMed:20709785}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:17416898,
CC ECO:0000269|PubMed:18375812}. Membrane {ECO:0000305}; Lipid-anchor,
CC GPI-anchor {ECO:0000305}.
CC -!- INDUCTION: Expressed in white cells. Transcription is regulated by the
CC transcription factor EFG1 and up-regulated by alpha-pheromone, by host
CC serum, in biofilms, and in high iron conditions. Repressed by BCR1 in
CC a/a biofilms. {ECO:0000269|PubMed:14665461,
CC ECO:0000269|PubMed:15387822, ECO:0000269|PubMed:17416898,
CC ECO:0000269|PubMed:19798425, ECO:0000269|PubMed:21566087,
CC ECO:0000269|PubMed:23563485, ECO:0000269|PubMed:23704884,
CC ECO:0000269|PubMed:23948468}.
CC -!- DOMAIN: The N-terminal domain mediates haploid invasive growth and
CC yeast cell-cell adhesion. {ECO:0000269|PubMed:18375812}.
CC -!- DOMAIN: The regions containing tandem repeats are required to project
CC the N-terminal region into the extracellular environment and to mediate
CC adhesion to polystyrene and to host cells. In allele CaO19.8979, the N-
CC terminal tandem repeat region is expended and contains up to 90 repeats
CC and the C-terminal tandem repeat region has one more repeat.
CC {ECO:0000269|PubMed:18375812}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC -!- SIMILARITY: Belongs to the PGA18 family. {ECO:0000305}.
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DR EMBL; CP017624; AOW27933.1; -; Genomic_DNA.
DR RefSeq; XP_714572.2; XM_709479.2.
DR AlphaFoldDB; G1UBC2; -.
DR BioGRID; 1226778; 11.
DR GeneID; 3643814; -.
DR KEGG; cal:CAALFM_C209530WA; -.
DR CGD; CAL0000175727; EAP1.
DR VEuPathDB; FungiDB:C2_09530W_A; -.
DR HOGENOM; CLU_280330_0_0_1; -.
DR OrthoDB; 1844974at2759; -.
DR PHI-base; PHI:517; -.
DR PRO; PR:G1UBC2; -.
DR Proteomes; UP000000559; Chromosome 2.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0007155; P:cell adhesion; IEA:UniProtKB-KW.
DR InterPro; IPR025928; Flocculin_t3_rpt.
DR Pfam; PF13928; Flocculin_t3; 2.
PE 1: Evidence at protein level;
KW Cell adhesion; Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Repeat; Secreted; Signal; Virulence.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..632
FT /note="Cell wall adhesin EAP1"
FT /id="PRO_0000424809"
FT PROPEP 633..653
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000424810"
FT REPEAT 147..152
FT /note="1-1"
FT REPEAT 153..158
FT /note="1-2"
FT REPEAT 159..164
FT /note="1-3"
FT REPEAT 165..170
FT /note="1-4"
FT REPEAT 171..176
FT /note="1-5"
FT REPEAT 177..182
FT /note="1-6"
FT REPEAT 192..197
FT /note="1-7"
FT REPEAT 198..203
FT /note="1-8"
FT REPEAT 204..209
FT /note="1-9"
FT REPEAT 210..215
FT /note="1-10"
FT REPEAT 216..221
FT /note="1-11"
FT REPEAT 222..227
FT /note="1-12"
FT REPEAT 228..233
FT /note="1-13"
FT REPEAT 234..248
FT /note="1-14"
FT REPEAT 249..254
FT /note="1-15"
FT REPEAT 326..345
FT /note="2-1"
FT REPEAT 346..365
FT /note="2-2"
FT REPEAT 366..389
FT /note="2-3"
FT REPEAT 390..413
FT /note="2-4"
FT REPEAT 414..433
FT /note="2-5"
FT REPEAT 434..457
FT /note="2-6"
FT REPEAT 458..477
FT /note="2-7"
FT REPEAT 478..501
FT /note="2-8"
FT REPEAT 502..521
FT /note="2-9"
FT REPEAT 522..541
FT /note="2-10"
FT REGION 19..146
FT /note="N-terminal cell-cell adhesion domain"
FT REGION 144..267
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 147..254
FT /note="15 X 6 AA tandem repeats, Ser/Thr-rich"
FT REGION 324..540
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 326..541
FT /note="10 X 20 AA approximate tandem repeats"
FT REGION 572..595
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 324..343
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 358..411
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 426..455
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 470..499
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 514..540
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 578..595
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 632
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
FT CARBOHYD 323
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 653 AA; 65265 MW; 62847FE0F3135DC1 CRC64;
MKVSQILPLA GAISVASGFW IPDFSNKQNS NSYPGQYKGK GGYQDDCGDD YKKGYKSKTY
SKVKPITSTD CTTPIQPTGT TTGYTKDVVE STSYTTDTAY TTTVITVTKC DGGSCSHTAV
TTGVTIITVT TNDVITEYTT YCPLTSTPAT ESTPATESTP ATESTPATES TPATESTPAT
ESTPCTTSTE TTPATESTPA TESTPATEST PATESTPATE STPATESTPA TESTPATEST
PCTTSTETTP ATESTASTET ASSTPVESTV IVPSTTVITV SSCYEDKCSV SSVTTGVVTI
SSEETIYTTY CPITSSITIP VPNTSTPAAP GTPVESQPVI PGTETTPAAP GTPVESQPVI
PGTETTPAAP GTPVESQPAT TPVAPGTETT PAAPGTPVES QPATTPVAPG TETTPAAPGT
PVESQPVIPG TETTPAAPGT PVESQPATTP VAPGTETTPA APGTPVESQP VIPGTETTPA
APGTPVESQP ATTPVAPGTE TTPAAPGTPV ESQPVIPGTE TTPAAPGTPG TEATPVTTQP
VSVLSTSQVV TASGEFSTVT AHSTSIVASC PEGGCVPEGQ QTETSPSVPT NGPEVEASSS
VLSIPVSSVT TSTIASSSET SVPPAQVSTF EGSGSALKKP YYGLAVAALV YFM
