PGA59_CANAL
ID PGA59_CANAL Reviewed; 113 AA.
AC Q5AF39; A0A1D8PLI6;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 25-MAY-2022, entry version 69.
DE RecName: Full=Cell wall protein PGA59;
DE AltName: Full=GPI-anchored protein 59;
DE Flags: Precursor;
GN Name=PGA59; OrderedLocusNames=CAALFM_C402370CA;
GN ORFNames=CaO19.10283, CaO19.2767;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP PREDICTION OF GPI-ANCHOR.
RX PubMed=12845604; DOI=10.1002/yea.1007;
RA De Groot P.W., Hellingwerf K.J., Klis F.M.;
RT "Genome-wide identification of fungal GPI proteins.";
RL Yeast 20:781-796(2003).
RN [5]
RP INDUCTION.
RX PubMed=15814841; DOI=10.1091/mbc.e05-01-0071;
RA Garcia-Sanchez S., Mavor A.L., Russell C.L., Argimon S., Dennison P.,
RA Enjalbert B., Brown A.J.;
RT "Global roles of Ssn6 in Tup1- and Nrg1-dependent gene regulation in the
RT fungal pathogen, Candida albicans.";
RL Mol. Biol. Cell 16:2913-2925(2005).
RN [6]
RP INDUCTION.
RX PubMed=16455273; DOI=10.1016/j.fgb.2005.12.002;
RA Castillo L., Martinez A.I., Garcera A., Garcia-Martinez J.,
RA Ruiz-Herrera J., Valentin E., Sentandreu R.;
RT "Genomic response programs of Candida albicans following protoplasting and
RT regeneration.";
RL Fungal Genet. Biol. 43:124-134(2006).
RN [7]
RP INDUCTION.
RX PubMed=19032986; DOI=10.1016/j.fgb.2008.10.012;
RA Walker L.A., Maccallum D.M., Bertram G., Gow N.A., Odds F.C., Brown A.J.;
RT "Genome-wide analysis of Candida albicans gene expression patterns during
RT infection of the mammalian kidney.";
RL Fungal Genet. Biol. 46:210-219(2009).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=19687206; DOI=10.1128/iai.00223-09;
RA Enjalbert B., Rachini A., Vediyappan G., Pietrella D., Spaccapelo R.,
RA Vecchiarelli A., Brown A.J., d'Enfert C.;
RT "A multifunctional, synthetic Gaussia princeps luciferase reporter for live
RT imaging of Candida albicans infections.";
RL Infect. Immun. 77:4847-4858(2009).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, GLYCOSYLATION,
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19383685; DOI=10.1099/mic.0.028902-0;
RA Moreno-Ruiz E., Ortu G., de Groot P.W., Cottier F., Loussert C.,
RA Prevost M.C., de Koster C., Klis F.M., Goyard S., d'Enfert C.;
RT "The GPI-modified proteins Pga59 and Pga62 of Candida albicans are required
RT for cell wall integrity.";
RL Microbiology 155:2004-2020(2009).
RN [10]
RP INDUCTION.
RX PubMed=21592964; DOI=10.1074/jbc.m111.233569;
RA Singh R.P., Prasad H.K., Sinha I., Agarwal N., Natarajan K.;
RT "Cap2-HAP complex is a critical transcriptional regulator that has dual but
RT contrasting roles in regulation of iron homeostasis in Candida albicans.";
RL J. Biol. Chem. 286:25154-25170(2011).
CC -!- FUNCTION: Cell wall protein necessary for cell wall integrity. Plays
CC only a minor role in hyphal morphogenesis and is not critical to
CC biofilm formation. {ECO:0000269|PubMed:19383685}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:19383685,
CC ECO:0000269|PubMed:19687206}. Membrane {ECO:0000305}; Lipid-anchor,
CC GPI-anchor {ECO:0000305}.
CC -!- INDUCTION: Up-regulated during cell wall regeneration and down-
CC regulated in kidney lesions. Expression is also regulated by HAP43 and
CC SSN6. {ECO:0000269|PubMed:15814841, ECO:0000269|PubMed:16455273,
CC ECO:0000269|PubMed:19032986, ECO:0000269|PubMed:21592964}.
CC -!- PTM: N- and O-glycosylated. {ECO:0000269|PubMed:19383685}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC -!- DISRUPTION PHENOTYPE: Leads to an altered cell wall structure with a
CC less electron dense inner layer of the cell wall, and a disorganized
CC outer layer. {ECO:0000269|PubMed:19383685}.
CC -!- SIMILARITY: Belongs to the HWP1 family. {ECO:0000305}.
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DR EMBL; CP017626; AOW29007.1; -; Genomic_DNA.
DR RefSeq; XP_720517.1; XM_715424.2.
DR AlphaFoldDB; Q5AF39; -.
DR STRING; 237561.Q5AF39; -.
DR GeneID; 3637881; -.
DR KEGG; cal:CAALFM_C402370CA; -.
DR CGD; CAL0000185470; PGA59.
DR VEuPathDB; FungiDB:C4_02370C_A; -.
DR eggNOG; ENOG502S42T; Eukaryota.
DR HOGENOM; CLU_105987_0_0_1; -.
DR InParanoid; Q5AF39; -.
DR OMA; SAHANVT; -.
DR PRO; PR:Q5AF39; -.
DR Proteomes; UP000000559; Chromosome 4.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009277; C:fungal-type cell wall; IDA:CGD.
DR GO; GO:0036244; P:cellular response to neutral pH; IMP:CGD.
DR GO; GO:0030447; P:filamentous growth; IMP:CGD.
DR GO; GO:0036178; P:filamentous growth of a population of unicellular organisms in response to neutral pH; IMP:CGD.
DR GO; GO:0031505; P:fungal-type cell wall organization; IDA:CGD.
DR InterPro; IPR025928; Flocculin_t3_rpt.
DR Pfam; PF13928; Flocculin_t3; 1.
PE 1: Evidence at protein level;
KW Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..92
FT /note="Cell wall protein PGA59"
FT /id="PRO_0000424731"
FT PROPEP 93..113
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000424732"
FT LIPID 92
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
FT CARBOHYD 22
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 80
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 113 AA; 11249 MW; C80B96A6C0335FF9 CRC64;
MQFSSAIILS AVAGSALATY ANSTVTDIAT TVVTITSCEE NKCHETEVTT GVTTVTEVET
TYTTYCPLPT AKAPVASTSN STTTPPVSTA EGAAAANAVP AVAAGLLALG AFM
