PGA62_CANAL
ID PGA62_CANAL Reviewed; 213 AA.
AC Q5AF41; A0A1D8PLK8;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=Cell wall protein PGA62;
DE AltName: Full=Flocculation protein 1;
DE AltName: Full=GPI-anchored protein 62;
DE Flags: Precursor;
GN Name=PGA62; Synonyms=FLO1; OrderedLocusNames=CAALFM_C402390WA;
GN ORFNames=CaO19.10281, CaO19.2765;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP PREDICTION OF GPI-ANCHOR.
RX PubMed=12845604; DOI=10.1002/yea.1007;
RA De Groot P.W., Hellingwerf K.J., Klis F.M.;
RT "Genome-wide identification of fungal GPI proteins.";
RL Yeast 20:781-796(2003).
RN [5]
RP INDUCTION.
RX PubMed=14555484; DOI=10.1128/ec.2.5.1018-1024.2003;
RA Chauhan N., Inglis D., Roman E., Pla J., Li D., Calera J.A., Calderone R.;
RT "Candida albicans response regulator gene SSK1 regulates a subset of genes
RT whose functions are associated with cell wall biosynthesis and adaptation
RT to oxidative stress.";
RL Eukaryot. Cell 2:1018-1024(2003).
RN [6]
RP INDUCTION.
RX PubMed=15269278; DOI=10.1091/mbc.e04-02-0144;
RA Harcus D., Nantel A., Marcil A., Rigby T., Whiteway M.;
RT "Transcription profiling of cyclic AMP signaling in Candida albicans.";
RL Mol. Biol. Cell 15:4490-4499(2004).
RN [7]
RP INDUCTION.
RX PubMed=15387822; DOI=10.1111/j.1365-2958.2004.04214.x;
RA Lan C.Y., Rodarte G., Murillo L.A., Jones T., Davis R.W., Dungan J.,
RA Newport G., Agabian N.;
RT "Regulatory networks affected by iron availability in Candida albicans.";
RL Mol. Microbiol. 53:1451-1469(2004).
RN [8]
RP INDUCTION.
RX PubMed=16455273; DOI=10.1016/j.fgb.2005.12.002;
RA Castillo L., Martinez A.I., Garcera A., Garcia-Martinez J.,
RA Ruiz-Herrera J., Valentin E., Sentandreu R.;
RT "Genomic response programs of Candida albicans following protoplasting and
RT regeneration.";
RL Fungal Genet. Biol. 43:124-134(2006).
RN [9]
RP INDUCTION.
RX PubMed=19089303; DOI=10.1111/j.1745-7270.2008.00483.x;
RA Yan L., Zhang J., Li M., Cao Y., Xu Z., Cao Y., Gao P., Wang Y., Jiang Y.;
RT "DNA microarray analysis of fluconazole resistance in a laboratory Candida
RT albicans strain.";
RL Acta Biochim. Biophys. Sin. 40:1048-1060(2008).
RN [10]
RP INDUCTION.
RX PubMed=18342603; DOI=10.1016/j.molcel.2008.02.006;
RA Hogues H., Lavoie H., Sellam A., Mangos M., Roemer T., Purisima E.,
RA Nantel A., Whiteway M.;
RT "Transcription factor substitution during the evolution of fungal ribosome
RT regulation.";
RL Mol. Cell 29:552-562(2008).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX PubMed=19555771; DOI=10.1016/j.fgb.2009.06.005;
RA Maddi A., Bowman S.M., Free S.J.;
RT "Trifluoromethanesulfonic acid-based proteomic analysis of cell wall and
RT secreted proteins of the ascomycetous fungi Neurospora crassa and Candida
RT albicans.";
RL Fungal Genet. Biol. 46:768-781(2009).
RN [12]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, GLYCOSYLATION,
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19383685; DOI=10.1099/mic.0.028902-0;
RA Moreno-Ruiz E., Ortu G., de Groot P.W., Cottier F., Loussert C.,
RA Prevost M.C., de Koster C., Klis F.M., Goyard S., d'Enfert C.;
RT "The GPI-modified proteins Pga59 and Pga62 of Candida albicans are required
RT for cell wall integrity.";
RL Microbiology 155:2004-2020(2009).
CC -!- FUNCTION: Cell wall protein necessary for cell wall integrity. plays
CC only a minor role in hyphal morphogenesis and is not critical to
CC biofilm formation. {ECO:0000269|PubMed:19383685}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000269|PubMed:19383685,
CC ECO:0000269|PubMed:19555771}. Membrane {ECO:0000305}; Lipid-anchor,
CC GPI-anchor {ECO:0000305}.
CC -!- INDUCTION: Induced during cell well regeneration, by fluconazole, and
CC in high iron conditions. Expression is also induced by TBF1 and
CC repressed by SSK1, and CYR1 or RAS1. {ECO:0000269|PubMed:14555484,
CC ECO:0000269|PubMed:15269278, ECO:0000269|PubMed:15387822,
CC ECO:0000269|PubMed:16455273, ECO:0000269|PubMed:18342603,
CC ECO:0000269|PubMed:19089303}.
CC -!- PTM: N- and O-glycosylated. {ECO:0000269|PubMed:19383685}.
CC -!- PTM: The GPI-anchor is attached to the protein in the endoplasmic
CC reticulum and serves to target the protein to the cell surface. There,
CC the glucosamine-inositol phospholipid moiety is cleaved off and the
CC GPI-modified mannoprotein is covalently attached via its lipidless GPI
CC glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.
CC -!- DISRUPTION PHENOTYPE: Leads to an altered cell wall structure with a
CC less electron dense inner layer of the cell wall, and a disorganized
CC outer layer. {ECO:0000269|PubMed:19383685}.
CC -!- SIMILARITY: Belongs to the HWP1 family. {ECO:0000305}.
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DR EMBL; CP017626; AOW29008.1; -; Genomic_DNA.
DR RefSeq; XP_720515.1; XM_715422.1.
DR AlphaFoldDB; Q5AF41; -.
DR GeneID; 3637879; -.
DR KEGG; cal:CAALFM_C402390WA; -.
DR CGD; CAL0000178718; PGA62.
DR VEuPathDB; FungiDB:C4_02390W_A; -.
DR eggNOG; ENOG502SDE4; Eukaryota.
DR HOGENOM; CLU_105987_0_0_1; -.
DR InParanoid; Q5AF41; -.
DR OMA; YCPLSTV; -.
DR PRO; PR:Q5AF41; -.
DR Proteomes; UP000000559; Chromosome 4.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0009277; C:fungal-type cell wall; IDA:CGD.
DR GO; GO:0030446; C:hyphal cell wall; IDA:CGD.
DR GO; GO:0031505; P:fungal-type cell wall organization; IDA:CGD.
DR InterPro; IPR025928; Flocculin_t3_rpt.
DR Pfam; PF13928; Flocculin_t3; 2.
PE 1: Evidence at protein level;
KW Cell wall; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT CHAIN 19..194
FT /note="Cell wall protein PGA62"
FT /id="PRO_0000424733"
FT PROPEP 195..213
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000424734"
FT REGION 120..194
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 137..187
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 194
FT /note="GPI-anchor amidated glycine"
FT /evidence="ECO:0000255"
FT CARBOHYD 22
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 213 AA; 21255 MW; 6EB880A0EA0AB03E CRC64;
MQFSSAVVLS AVAGSALAAY SNSTVTDIQT TVVTITSCEE NKCHETEVTT GVTTVTEVDT
TYTTYCPLST TEAPAPSTAT DVSTTVVTIT SCEEDKCHET AVTTGVTTVT EGTTIYTTYC
PLPSTEAPGP APSTAEESKP AESSPVPTTA AESSPAKTTA AESSPAQETT PKTVAAESSS
AETTAPAVST AEAGAAANAV PVAAGLLALA ALF
