ID   PGAA_ECO57              Reviewed;         807 AA.
AC   P69435; P75907;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Poly-beta-1,6-N-acetyl-D-glucosamine export protein;
DE            Short=PGA export protein;
DE            Short=Poly-beta-1,6-GlcNAc export protein;
DE   Flags: Precursor;
GN   Name=pgaA; OrderedLocusNames=Z1526, ECs1270;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Exports the biofilm adhesin polysaccharide poly-beta-1,6-N-
CC       acetyl-D-glucosamine (PGA) across the outer membrane. The PGA
CC       transported seems to be partially N-deacetylated since N-deacetylation
CC       of PGA by PgaB is needed for PGA export through the PgaA porin (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- DOMAIN: Contains a predicted C-terminal beta-barrel porin domain and a
CC       N-terminal periplasmic superhelical domain containing tetratricopeptide
CC       repeats, which may mediate protein-protein interactions, perhaps with
CC       PgaB. {ECO:0000250}.
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DR   EMBL; AE005174; AAG55642.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB34693.1; -; Genomic_DNA.
DR   PIR; F90787; F90787.
DR   RefSeq; NP_309297.1; NC_002695.1.
DR   RefSeq; WP_000287458.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P69435; -.
DR   SMR; P69435; -.
DR   STRING; 155864.EDL933_1451; -.
DR   EnsemblBacteria; AAG55642; AAG55642; Z1526.
DR   EnsemblBacteria; BAB34693; BAB34693; ECs_1270.
DR   GeneID; 912850; -.
DR   KEGG; ece:Z1526; -.
DR   KEGG; ecs:ECs_1270; -.
DR   PATRIC; fig|386585.9.peg.1378; -.
DR   eggNOG; COG0457; Bacteria.
DR   HOGENOM; CLU_018289_0_0_6; -.
DR   OMA; WDKRPYD; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:1901515; F:poly-beta-1,6-N-acetyl-D-glucosamine transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.25.40.10; -; 2.
DR   InterPro; IPR023870; PGA_export_porin_PgaA.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   SMART; SM00028; TPR; 1.
DR   SUPFAM; SSF48452; SSF48452; 2.
DR   TIGRFAMs; TIGR03939; PGA_TPR_OMP; 1.
DR   PROSITE; PS50005; TPR; 1.
DR   PROSITE; PS50293; TPR_REGION; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Membrane; Reference proteome; Repeat; Signal;
KW   TPR repeat; Transmembrane; Transmembrane beta strand; Transport.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..807
FT                   /note="Poly-beta-1,6-N-acetyl-D-glucosamine export protein"
FT                   /id="PRO_0000035695"
FT   REPEAT          98..131
FT                   /note="TPR 1"
FT   REPEAT          165..198
FT                   /note="TPR 2"
FT   REPEAT          279..311
FT                   /note="TPR 3"
SQ   SEQUENCE   807 AA;  92207 MW;  B20067C3D41723FD CRC64;
     MYSSSRKRCP KTKWALKLLT AAFLAASPAA KSAVNNAYDA LIIEARKGNT QPALSWFALK
     SALSNNQIAD WLQIALWAGQ DKQVITVYNR YRHQQLPARG YAAVAVAYRN LQQWQNSLTL
     WQKALSLEPQ NKDYQRGQIL TLADAGHYDT ALVKLKQLNS GAPDKANLLA EAYIYKLAGR
     HQDELRAMTE SLPENASTQQ YPTEYVQALR NNQLAAAIDD ANLTPDIRAD IHAELVRLSF
     MPTRSESERY AIADRALAQY AALEILWHDN PDRTAQYQRI QVDHLGALLT RDRYKDVISH
     YQRLKKTGQI IPPWGQYWVA SAYLKDHQPK KAQSIMTELF YHKETIAPDL SDEELADLFY
     SHLESENYPG ALTVTQHTIN TSPPFLRLMG TPTSIPNDTW LQGHSFLSTV AKYSNDLPQA
     EMTARELAYN APGNQGLRID YASVLQARGW PRAAENELKK AEVIEPRNIN LEVEQAWTAL
     TLQEWQQAAV LTHDVVEREP QDPGVVRLKR AVDVHNLAEL RIAGSTGIDA EGPDSGKHDV
     DLTTIVYSPP LKDNWRGFAG FGYADGQFSE GKGIVRDWLA GVEWRSRNIW LEAEYAERVF
     NHEHKPGARL SGWYDFNDNW RIGSQLERLS HRVPLRAMKN GVTGNSAQAY VRWYQNERRK
     YGVSWAFTDF SDSNQRHEVS LEGQERIWSS PYLIVDFLPS LYYEQNTEHD TPYYNPIKTF
     DIVPAFEASH LLWRSYENSW EQIFSAGVGA SWQKHYGTDV VTQLGYGQRI SWNDVIDAGA
     TLRWEKRPYD GDREHNLYVE FDMTFRF