ID   PGAD_ECO57              Reviewed;         137 AA.
AC   P69433; P75904;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=Biofilm PGA synthesis protein PgaD;
GN   Name=pgaD; OrderedLocusNames=Z1523, ECs1267;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Required for the synthesis of poly-beta-1,6-N-acetyl-D-
CC       glucosamine (PGA), a biofilm adhesin polysaccharide. May assist the
CC       glycosyltransferase PgaC in the polymerization of PGA (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
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DR   EMBL; AE005174; AAG55639.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB34690.1; -; Genomic_DNA.
DR   PIR; C85647; C85647.
DR   PIR; C90787; C90787.
DR   RefSeq; NP_309294.1; NC_002695.1.
DR   RefSeq; WP_001061095.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P69433; -.
DR   STRING; 155864.EDL933_1448; -.
DR   EnsemblBacteria; AAG55639; AAG55639; Z1523.
DR   EnsemblBacteria; BAB34690; BAB34690; ECs_1267.
DR   GeneID; 58391625; -.
DR   GeneID; 912758; -.
DR   KEGG; ece:Z1523; -.
DR   KEGG; ecs:ECs_1267; -.
DR   PATRIC; fig|386585.9.peg.1375; -.
DR   eggNOG; COG3658; Bacteria.
DR   HOGENOM; CLU_131509_0_0_6; -.
DR   OMA; VFWESEA; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043709; P:cell adhesion involved in single-species biofilm formation; IEA:InterPro.
DR   InterPro; IPR023829; PGA_PgaD.
DR   Pfam; PF13994; PgaD; 1.
DR   TIGRFAMs; TIGR03940; PGA_PgaD; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..137
FT                   /note="Biofilm PGA synthesis protein PgaD"
FT                   /id="PRO_0000058348"
FT   TOPO_DOM        1..23
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        24..44
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        45..53
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        75..137
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   137 AA;  16082 MW;  574776B68F667D5D CRC64;
     MNNLIITTRQ SPVRLLVDYV ATTILWTLFA LFIFLFAMDL LTGYYWQSEA RSRLQFYFLL
     AVANAVVLIV WALYNKLRFQ KQQHHAAYQY TPQEYAESLA IPDELYQQLQ KSHRMSVHFT
     SQGQIKMVVS EKALVRA