PGAP3_DROME
ID PGAP3_DROME Reviewed; 326 AA.
AC Q7K0P4; A1Z6N0;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 2.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Post-GPI attachment to proteins factor 3;
DE Flags: Precursor;
GN Name=PGAP3; ORFNames=CG3271;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-42, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=Oregon-R; TISSUE=Head;
RX PubMed=17893096; DOI=10.1093/glycob/cwm097;
RA Koles K., Lim J.-M., Aoki K., Porterfield M., Tiemeyer M., Wells L.,
RA Panin V.;
RT "Identification of N-glycosylated proteins from the central nervous system
RT of Drosophila melanogaster.";
RL Glycobiology 17:1388-1403(2007).
CC -!- FUNCTION: Involved in the lipid remodeling steps of GPI-anchor
CC maturation. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7K0P4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7K0P4-2; Sequence=VSP_034160;
CC -!- SIMILARITY: Belongs to the PGAP3 family. {ECO:0000305}.
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DR EMBL; AE013599; AAM70807.1; -; Genomic_DNA.
DR EMBL; AE013599; AAM70808.1; -; Genomic_DNA.
DR EMBL; AY069668; AAL39813.1; -; mRNA.
DR RefSeq; NP_610223.1; NM_136379.4. [Q7K0P4-2]
DR RefSeq; NP_724485.1; NM_165482.4. [Q7K0P4-1]
DR AlphaFoldDB; Q7K0P4; -.
DR IntAct; Q7K0P4; 5.
DR STRING; 7227.FBpp0085453; -.
DR TCDB; 9.B.132.1.1; the post-gpi attachment protein-3 (p-gap3) family.
DR GlyGen; Q7K0P4; 1 site.
DR iPTMnet; Q7K0P4; -.
DR PaxDb; Q7K0P4; -.
DR PRIDE; Q7K0P4; -.
DR DNASU; 35570; -.
DR EnsemblMetazoa; FBtr0086118; FBpp0085452; FBgn0033088. [Q7K0P4-1]
DR EnsemblMetazoa; FBtr0086119; FBpp0085453; FBgn0033088. [Q7K0P4-2]
DR GeneID; 35570; -.
DR KEGG; dme:Dmel_CG3271; -.
DR UCSC; CG3271-RA; d. melanogaster. [Q7K0P4-1]
DR CTD; 93210; -.
DR FlyBase; FBgn0033088; PGAP3.
DR VEuPathDB; VectorBase:FBgn0033088; -.
DR eggNOG; KOG2970; Eukaryota.
DR GeneTree; ENSGT00390000001304; -.
DR HOGENOM; CLU_032917_1_1_1; -.
DR InParanoid; Q7K0P4; -.
DR OMA; FMIEDCR; -.
DR PhylomeDB; Q7K0P4; -.
DR BioGRID-ORCS; 35570; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35570; -.
DR PRO; PR:Q7K0P4; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033088; Expressed in oviduct (Drosophila) and 24 other tissues.
DR Genevisible; Q7K0P4; DM.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0031227; C:intrinsic component of endoplasmic reticulum membrane; ISS:UniProtKB.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; ISS:UniProtKB.
DR GO; GO:0006506; P:GPI anchor biosynthetic process; IBA:GO_Central.
DR GO; GO:0006505; P:GPI anchor metabolic process; ISS:UniProtKB.
DR InterPro; IPR007217; Per1-like.
DR PANTHER; PTHR13148; PTHR13148; 1.
DR Pfam; PF04080; Per1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Glycoprotein; Golgi apparatus;
KW GPI-anchor biosynthesis; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..326
FT /note="Post-GPI attachment to proteins factor 3"
FT /id="PRO_0000339361"
FT TOPO_DOM 23..105
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 127..142
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 164..171
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..203
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..232
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 254..267
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 289..302
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 42
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17893096"
FT VAR_SEQ 307..326
FT /note="ECVEILLYSNIATCVCILAS -> DFMIEDCRTLRKEKAAAGGYSFYN (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_034160"
SQ SEQUENCE 326 AA; 38113 MW; F838F716FD0742FE CRC64;
MSSRSLSAIV LLLGALVTAC LASNGDRTQF FHNCRQNCER TNCSADGLEI QEQAVKFYQQ
SVFDRLFQWS CADECQYGCM WRTVFAFFER GWPIPQFYGK WPFLRLLGMQ EPASVIFSCL
NFVVHLRLLR KFRREVRPDS PCYMLTHIFA VTSLNGWIWS AIFHTRDFPL TELLDYAFAY
SIILCSLYVM VMRMLHRYSL FLRGVITLAF LSYYINYFAY LSVGRFNYAF NMMVNVATGV
IAAVGWFVWC HFVRTRRPYF RRILRFYILM ALAMSLELLD FPPILWILDA HALWHLATIP
LASLYYECVE ILLYSNIATC VCILAS
