PGBD5_MOUSE
ID PGBD5_MOUSE Reviewed; 523 AA.
AC D3YZI9; D3Z077; E9PUA8; Q52KE6;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=PiggyBac transposable element-derived protein 5;
DE EC=3.1.-.-;
DE AltName: Full=PiggyBac transposase 5;
GN Name=Pgbd5 {ECO:0000312|MGI:MGI:2429955};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-520, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MISCELLANEOUS.
RX PubMed=24180413; DOI=10.1186/1759-8753-4-23;
RA Pavelitz T., Gray L.T., Padilla S.L., Bailey A.D., Weiner A.M.;
RT "PGBD5: a neural-specific intron-containing piggyBac transposase
RT domesticated over 500 million years ago and conserved from cephalochordates
RT to humans.";
RL Mob. DNA 4:23-23(2013).
CC -!- FUNCTION: Transposase that mediates sequence-specific genomic
CC rearrangements. {ECO:0000250|UniProtKB:Q8N414}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:24180413}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=D3YZI9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=D3YZI9-2; Sequence=VSP_059132;
CC -!- TISSUE SPECIFICITY: Expressed in the brain (at protein level).
CC {ECO:0000269|PubMed:24180413}.
CC -!- MISCELLANEOUS: Has been domesticated very early in vertebrate
CC evolution, approximately 500 million years ago, in the common ancestor
CC of cephalochordates and vertebrates. {ECO:0000305|PubMed:24180413}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH94384.1; Type=Miscellaneous discrepancy; Note=20-nucleotide gap within coding exon 1.; Evidence={ECO:0000305};
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DR EMBL; AC114005; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC126930; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC094384; AAH94384.1; ALT_SEQ; mRNA.
DR CCDS; CCDS22770.1; -. [D3YZI9-2]
DR RefSeq; NP_741958.1; NM_171824.2. [D3YZI9-2]
DR RefSeq; XP_006530867.1; XM_006530804.1.
DR AlphaFoldDB; D3YZI9; -.
DR SMR; D3YZI9; -.
DR IntAct; D3YZI9; 1.
DR MINT; D3YZI9; -.
DR STRING; 10090.ENSMUSP00000120984; -.
DR iPTMnet; D3YZI9; -.
DR PhosphoSitePlus; D3YZI9; -.
DR MaxQB; D3YZI9; -.
DR PaxDb; D3YZI9; -.
DR PeptideAtlas; D3YZI9; -.
DR PRIDE; D3YZI9; -.
DR ProteomicsDB; 288104; -. [D3YZI9-1]
DR ProteomicsDB; 288105; -. [D3YZI9-2]
DR Antibodypedia; 54100; 36 antibodies from 12 providers.
DR DNASU; 209966; -.
DR Ensembl; ENSMUST00000052580; ENSMUSP00000054788; ENSMUSG00000050751. [D3YZI9-2]
DR Ensembl; ENSMUST00000136892; ENSMUSP00000123265; ENSMUSG00000050751. [D3YZI9-2]
DR Ensembl; ENSMUST00000140012; ENSMUSP00000120984; ENSMUSG00000050751. [D3YZI9-1]
DR GeneID; 209966; -.
DR KEGG; mmu:209966; -.
DR UCSC; uc009nxb.1; mouse.
DR CTD; 79605; -.
DR MGI; MGI:2429955; Pgbd5.
DR VEuPathDB; HostDB:ENSMUSG00000050751; -.
DR eggNOG; ENOG502QT9F; Eukaryota.
DR GeneTree; ENSGT00510000047893; -.
DR InParanoid; D3YZI9; -.
DR OMA; SEAYHVT; -.
DR OrthoDB; 1139412at2759; -.
DR PhylomeDB; D3YZI9; -.
DR TreeFam; TF332005; -.
DR BioGRID-ORCS; 209966; 2 hits in 72 CRISPR screens.
DR PRO; PR:D3YZI9; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; D3YZI9; protein.
DR Bgee; ENSMUSG00000050751; Expressed in dentate gyrus of hippocampal formation and 153 other tissues.
DR ExpressionAtlas; D3YZI9; baseline and differential.
DR Genevisible; Q52KE6; MM.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0004803; F:transposase activity; ISS:UniProtKB.
DR GO; GO:0098038; P:non-replicative transposition, DNA-mediated; ISS:UniProtKB.
DR InterPro; IPR029526; PGBD.
DR InterPro; IPR042423; PGBD5.
DR PANTHER; PTHR28576; PTHR28576; 1.
DR Pfam; PF13843; DDE_Tnp_1_7; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Endonuclease; Hydrolase; Nuclease; Nucleus;
KW Phosphoprotein; Reference proteome; Transposition.
FT CHAIN 1..523
FT /note="PiggyBac transposable element-derived protein 5"
FT /id="PRO_0000441912"
FT REGION 30..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 41..57
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 520
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 1..114
FT /note="Missing (in isoform 2)"
FT /id="VSP_059132"
SQ SEQUENCE 523 AA; 58300 MW; F6F419B2DFAF5FB8 CRC64;
MAEGGGGSRR RAPALLEAAR ARYESLHISD DVFGESGPDS GGNPFYSTSA ASRSSSAASS
DDERERPAPP GTAPPSYAAD PLELEEDETG GGWSAVLRDR PSPRFEDTGG PTRKMPPSAS
AVDFFQLFVP DNVLKNMVVQ TNMYARKFQE RFGSDGAWVE VTLAEMKAFL GYVISTSVSH
CESVLSIWSG GFYSNRSLAL VMSQARFEKI LKYFHVVAFR SSQTTHGLYK VQPFLDSLQS
GFDAAFRPSQ TQVLHEPLID EDPVFIATCT ERELRKRKKR KFSLWVRQCS STGFIIQIYV
HLKEGGGPDG LDALKNKPQL HSMVARSLCR NAAGKNYIIF TGPSITSLNL FEEFEKQGIY
CCGLLSSRKS DCTGLPPSML TNPATPLARG QHQIRTKGNM SLICWYNKGH FRFLTNAYSP
VQKGVIIKRR SGEIPCPLAV EAFAAHLSYI CRYDDKYSKY FISHKPNKTW QQVFWFAISI
AVNNAYILYK MSDAYHVKKY SRAQFGERLV RELLGLEDSS PAH
