PGDS_BACSU
ID PGDS_BACSU Reviewed; 413 AA.
AC P96740; Q8L0S3;
DT 16-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 2.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Gamma-DL-glutamyl hydrolase;
DE EC=3.4.19.-;
DE AltName: Full=Poly-gamma-glutamate depolymerase;
DE Short=PGA depolymerase;
DE Flags: Precursor;
GN Name=pgdS; Synonyms=ywtD; OrderedLocusNames=BSU35860;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12073126; DOI=10.1007/s00253-002-0984-x;
RA Ashiuchi M., Misono H.;
RT "Biochemistry and molecular genetics of poly-gamma-glutamate synthesis.";
RL Appl. Microbiol. Biotechnol. 59:9-14(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=NBRC 16449;
RX PubMed=12644511; DOI=10.1128/jb.185.7.2379-2382.2003;
RA Suzuki T., Tahara Y.;
RT "Characterization of the Bacillus subtilis ywtD gene, whose product is
RT involved in gamma-polyglutamic acid degradation.";
RL J. Bacteriol. 185:2379-2382(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9353933; DOI=10.1099/00221287-143-10-3313;
RA Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V.,
RA Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G.,
RA Kunst F., Danchin A., Glaser P.;
RT "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334
RT degrees).";
RL Microbiology 143:3313-3328(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP PROTEIN SEQUENCE OF 33-43, AND SUBCELLULAR LOCATION.
RC STRAIN=168;
RX PubMed=10658653; DOI=10.1099/00221287-146-1-65;
RA Hirose I., Sano K., Shioda I., Kumano M., Nakamura K., Yamane K.;
RT "Proteome analysis of Bacillus subtilis extracellular proteins: a two-
RT dimensional protein electrophoretic study.";
RL Microbiology 146:65-75(2000).
RN [6]
RP SUBCELLULAR LOCATION, INDUCTION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=168;
RX PubMed=11987133;
RX DOI=10.1002/1615-9861(200205)2:5<591::aid-prot591>3.0.co;2-8;
RA Antelmann H., Yamamoto H., Sekiguchi J., Hecker M.;
RT "Stabilization of cell wall proteins in Bacillus subtilis: a proteomic
RT approach.";
RL Proteomics 2:591-602(2002).
CC -!- FUNCTION: Cleaves, in an endo-type manner, the gamma-glutamyl bond
CC between D-glutamate and L-glutamate of poly-gamma-glutamate (PGA).
CC -!- ACTIVITY REGULATION: Inhibited by pretreatment with 1 mM 4-
CC (hydroxymercuri)benzoate, a sulfhydryl inhibitor.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 5.0.;
CC Temperature dependence:
CC Optimum temperature is 45 degrees Celsius.;
CC -!- SUBCELLULAR LOCATION: Secreted. Secreted, cell wall. Note=Cell wall
CC localization shown in PubMed:11987133.
CC -!- INDUCTION: In stationary phase; under control of SigD.
CC {ECO:0000269|PubMed:11987133}.
CC -!- SIMILARITY: Belongs to the peptidase C40 family. {ECO:0000255|PROSITE-
CC ProRule:PRU01284, ECO:0000305}.
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DR EMBL; AB085821; BAB96576.1; -; Genomic_DNA.
DR EMBL; AB080748; BAC11878.1; -; Genomic_DNA.
DR EMBL; Z92954; CAB07476.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15603.1; -; Genomic_DNA.
DR PIR; B70070; B70070.
DR RefSeq; NP_391467.1; NC_000964.3.
DR RefSeq; WP_003242751.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P96740; -.
DR SMR; P96740; -.
DR STRING; 224308.BSU35860; -.
DR MEROPS; C40.005; -.
DR PaxDb; P96740; -.
DR PRIDE; P96740; -.
DR DNASU; 936837; -.
DR EnsemblBacteria; CAB15603; CAB15603; BSU_35860.
DR GeneID; 936837; -.
DR KEGG; bsu:BSU35860; -.
DR PATRIC; fig|224308.179.peg.3882; -.
DR eggNOG; COG0791; Bacteria.
DR OMA; MNTSTYW; -.
DR BioCyc; BSUB:BSU35860-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR InterPro; IPR000064; NLP_P60_dom.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR Pfam; PF00877; NLPC_P60; 3.
DR SUPFAM; SSF54001; SSF54001; 3.
DR PROSITE; PS51935; NLPC_P60; 3.
PE 1: Evidence at protein level;
KW Cell wall; Direct protein sequencing; Hydrolase; Protease;
KW Reference proteome; Secreted; Signal; Thiol protease.
FT SIGNAL 1..32
FT /evidence="ECO:0000269|PubMed:10658653"
FT CHAIN 33..413
FT /note="Gamma-DL-glutamyl hydrolase"
FT /id="PRO_0000019756"
FT DOMAIN 33..159
FT /note="NlpC/P60 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT DOMAIN 163..287
FT /note="NlpC/P60 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT DOMAIN 291..413
FT /note="NlpC/P60 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT ACT_SITE 194
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT ACT_SITE 247
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT ACT_SITE 259
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT VARIANT 112..114
FT /note="TGT -> NGS (in strain: IFO 16449)"
FT VARIANT 224
FT /note="V -> I (in strain: IFO 16449)"
FT VARIANT 354
FT /note="E -> K (in strain: IFO 16449)"
SQ SEQUENCE 413 AA; 45247 MW; F7801CE5749234FB CRC64;
MNTLANWKKF LLVAVIICFL VPIMTKAEIA EADTSSELIV SEAKNLLGYQ YKYGGETPKE
GFDPSGLIQY VFSKADIHLP RSVNDQYKIG TAVKPENLKP GDILFFKKEG STGTVPTHDA
LYIGDGQMVH STQSKGVIIT NYKKSSYWSG TYIGARRIAA DPATADVPVV QEAEKYIGVP
YVFGGSTPSE GFDCSGLVQY VFQQALGIYL PRSAEQQWAV GEKVAPQNIK PGDVVYFSNT
YKTGISHAGI YAGAGRFIQA SRSEKVTISY LSEDYWKSKM TGIRRFDNLT IPKENPIVSE
ATLYVGEVPY KQGGVTPETG FDTAGFVQYV YQKAAGISLP RYATSQYNAG TKIEKADLKP
GDIVFFQSTS LNPSIYIGNG QVVHVTLSNG VTITNMNTST YWKDKYAGSI RVQ
