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PGFS2_BOVIN
ID   PGFS2_BOVIN             Reviewed;         323 AA.
AC   P52897;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Prostaglandin F synthase 2;
DE            Short=PGF 2;
DE            Short=PGF synthase 2;
DE            Short=PGFS2;
DE            EC=1.1.1.188;
DE   AltName: Full=Prostaglandin F synthase II;
DE            Short=PGFSII;
DE   AltName: Full=Prostaglandin-D2 11 reductase 2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RX   PubMed=1339268; DOI=10.1016/s0006-291x(05)80323-7;
RA   Kuchinke W., Barski O., Watanabe K., Hayaishi O.;
RT   "A lung type prostaglandin F synthase is expressed in bovine liver: cDNA
RT   sequence and expression in E. coli.";
RL   Biochem. Biophys. Res. Commun. 183:1238-1246(1992).
CC   -!- FUNCTION: Catalyzes the reduction of PGD(2) and PGH(2) to PGF(2 alpha)
CC       and a stereoisomer, respectively. It has a broad substrate specificity
CC       and reduces also other carbonyl compounds.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NADP(+) + prostaglandin F2alpha = H(+) + NADPH + prostaglandin
CC         D2; Xref=Rhea:RHEA:10140, ChEBI:CHEBI:15378, ChEBI:CHEBI:57404,
CC         ChEBI:CHEBI:57406, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.1.1.188;
CC   -!- PATHWAY: Lipid metabolism; prostaglandin biosynthesis.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
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DR   EMBL; M86544; AAA30730.1; -; mRNA.
DR   PIR; E75572; JH0575.
DR   AlphaFoldDB; P52897; -.
DR   SMR; P52897; -.
DR   STRING; 9913.ENSBTAP00000024086; -.
DR   PaxDb; P52897; -.
DR   Ensembl; ENSBTAT00000024086; ENSBTAP00000024086; ENSBTAG00000022570.
DR   VEuPathDB; HostDB:ENSBTAG00000022570; -.
DR   eggNOG; KOG1577; Eukaryota.
DR   GeneTree; ENSGT00940000153677; -.
DR   HOGENOM; CLU_023205_0_0_1; -.
DR   InParanoid; P52897; -.
DR   OMA; RMNFPAR; -.
DR   TreeFam; TF106492; -.
DR   SABIO-RK; P52897; -.
DR   UniPathway; UPA00662; -.
DR   Proteomes; UP000009136; Chromosome 13.
DR   Bgee; ENSBTAG00000022570; Expressed in lung and 85 other tissues.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004032; F:alditol:NADP+ 1-oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0047023; F:androsterone dehydrogenase activity; IBA:GO_Central.
DR   GO; GO:0032052; F:bile acid binding; IBA:GO_Central.
DR   GO; GO:0047086; F:ketosteroid monooxygenase activity; IBA:GO_Central.
DR   GO; GO:0036131; F:prostaglandin D2 11-ketoreductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0047017; F:prostaglandin-F synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016229; F:steroid dehydrogenase activity; IBA:GO_Central.
DR   GO; GO:0044597; P:daunorubicin metabolic process; IBA:GO_Central.
DR   GO; GO:0044598; P:doxorubicin metabolic process; IBA:GO_Central.
DR   GO; GO:0042448; P:progesterone metabolic process; IBA:GO_Central.
DR   GO; GO:0001516; P:prostaglandin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006693; P:prostaglandin metabolic process; IBA:GO_Central.
DR   GO; GO:0008202; P:steroid metabolic process; IBA:GO_Central.
DR   CDD; cd19108; AKR_AKR1C1-35; 1.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR044482; AKR1C.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 1.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; SSF51430; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR   PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Fatty acid biosynthesis; Fatty acid metabolism;
KW   Lipid biosynthesis; Lipid metabolism; NADP; Oxidoreductase;
KW   Prostaglandin biosynthesis; Prostaglandin metabolism; Reference proteome.
FT   CHAIN           1..323
FT                   /note="Prostaglandin F synthase 2"
FT                   /id="PRO_0000124647"
FT   ACT_SITE        55
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         20..24
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         50
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         117
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         166..167
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         190
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         216..221
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         270..280
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   SITE            84
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   323 AA;  36742 MW;  21A5BE0668D07A2C CRC64;
     MDPKSQRVKF NDGHFIPVLG FGTYAPEEVP KSEALEATKF AIEVGFRHVD SAHLYQNEEQ
     VGQAIRSKIA DGTVKREDIF YTSKLWCNSL QPELVRPALE KSLQNLQLDY VDLYIIHSPV
     SLKPGNKFVP KDESGKLIFD SVDLCHTWEA LEKCKDAGLT KSIGVSNFNH KQLEKILNKP
     GLKYKPVCNQ VECHPYLNQS KLLEFCKSHD IVLVAYAALG AQLLSEWVNS NNPVLLEDPV
     LCAIAKKHKQ TPALVALRYQ VQRGVVVLAK SFNKKRIKEN MQVFDFELTP EDMKAIDGLN
     RNTRYYDFQQ GIGHPEYPFS EEY
 
 
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