PGIP2_ARATH
ID PGIP2_ARATH Reviewed; 330 AA.
AC Q9M5J8; Q9FL58;
DT 27-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 27-MAY-2002, sequence version 2.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Polygalacturonase inhibitor 2;
DE AltName: Full=Polygalacturonase-inhibiting protein 2;
DE Short=PGIP-2;
DE Flags: Precursor;
GN Name=PGIP2; OrderedLocusNames=At5g06870; ORFNames=MOJ9.4;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE OF 5-330.
RA Park B., Jin Y., Nam S., Kim H.;
RT "Arabidopsis thaliana polygalacturonase inhibiting protein 2 (PGIP2)
RT gene.";
RL Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Inhibitor of fungal polygalacturonase. It is an important
CC factor for plant resistance to phytopathogenic fungi.
CC {ECO:0000250|UniProtKB:P58822}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000250|UniProtKB:P58822}. Membrane; Peripheral membrane protein.
CC -!- SIMILARITY: Belongs to the polygalacturonase-inhibiting protein family.
CC {ECO:0000305}.
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DR EMBL; AB010697; BAB11145.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91077.1; -; Genomic_DNA.
DR EMBL; AY035121; AAK59626.1; -; mRNA.
DR EMBL; AY113916; AAM44964.1; -; mRNA.
DR EMBL; AF229250; AAF69828.1; -; mRNA.
DR RefSeq; NP_196305.1; NM_120770.4.
DR AlphaFoldDB; Q9M5J8; -.
DR SMR; Q9M5J8; -.
DR STRING; 3702.AT5G06870.1; -.
DR PaxDb; Q9M5J8; -.
DR PRIDE; Q9M5J8; -.
DR ProteomicsDB; 234995; -.
DR EnsemblPlants; AT5G06870.1; AT5G06870.1; AT5G06870.
DR GeneID; 830578; -.
DR Gramene; AT5G06870.1; AT5G06870.1; AT5G06870.
DR KEGG; ath:AT5G06870; -.
DR Araport; AT5G06870; -.
DR TAIR; locus:2169404; AT5G06870.
DR eggNOG; ENOG502QRQP; Eukaryota.
DR HOGENOM; CLU_000288_18_22_1; -.
DR InParanoid; Q9M5J8; -.
DR OMA; WNPDTDC; -.
DR OrthoDB; 1111550at2759; -.
DR PhylomeDB; Q9M5J8; -.
DR PRO; PR:Q9M5J8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9M5J8; baseline and differential.
DR Genevisible; Q9M5J8; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009505; C:plant-type cell wall; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0090353; F:polygalacturonase inhibitor activity; IDA:TAIR.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR013210; LRR_N_plant-typ.
DR Pfam; PF13855; LRR_8; 1.
DR Pfam; PF08263; LRRNT_2; 1.
PE 2: Evidence at transcript level;
KW Cell wall; Disulfide bond; Glycoprotein; Leucine-rich repeat; Membrane;
KW Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..330
FT /note="Polygalacturonase inhibitor 2"
FT /id="PRO_0000023883"
FT REPEAT 69..93
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 94..117
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 118..141
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 142..166
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 167..192
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 194..215
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 217..237
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 238..260
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 261..285
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 287..308
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT CARBOHYD 106
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 120
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 130
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 291
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 25..55
FT /evidence="ECO:0000250|UniProtKB:P58822"
FT DISULFID 56..63
FT /evidence="ECO:0000250|UniProtKB:P58822"
FT DISULFID 298..320
FT /evidence="ECO:0000250|UniProtKB:P58822"
FT DISULFID 322..329
FT /evidence="ECO:0000250|UniProtKB:P58822"
SQ SEQUENCE 330 AA; 37068 MW; 9911DF7015DDC280 CRC64;
MDKTMTLFLL LSTLLLTTSL AKDLCHKDDK TTLLKIKKSL NNPYHLASWD PKTDCCSWYC
LECGDATVNH RVTSLIIQDG EISGQIPPEV GDLPYLTSLI FRKLTNLTGH IQPTIAKLKN
LTFLRLSWTN LTGPVPEFLS QLKNLEYIDL SFNDLSGSIP SSLSSLRKLE YLELSRNKLT
GPIPESFGTF SGKVPSLFLS HNQLSGTIPK SLGNPDFYRI DLSRNKLQGD ASILFGAKKT
TWIVDISRNM FQFDLSKVKL AKTLNNLDMN HNGITGSIPA EWSKAYFQLL NVSYNRLCGR
IPKGEYIQRF DSYSFFHNKC LCGAPLPSCK
