ID   PGK_TETTH               Reviewed;         420 AA.
AC   P50313;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Phosphoglycerate kinase;
DE            EC=2.7.2.3;
GN   Name=PGK;
OS   Tetrahymena thermophila.
OC   Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC   Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC   Tetrahymena.
OX   NCBI_TaxID=5911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=CU329;
RX   PubMed=1602492; DOI=10.1007/bf00162995;
RA   Vohra G.B., Golding G.B., Tsao N., Pearlman R.E.;
RT   "A phylogenetic analysis based on the gene encoding phosphoglycerate
RT   kinase.";
RL   J. Mol. Evol. 34:383-395(1992).
RN   [2]
RP   ERRATUM OF PUBMED:1602492.
RX   PubMed=1487830;
RA   Vohra G.B., Golding G.B., Tsao N., Pearlman R.E.;
RL   J. Mol. Evol. 35:466-466(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl
CC         phosphate + ADP; Xref=Rhea:RHEA:14801, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57604, ChEBI:CHEBI:58272, ChEBI:CHEBI:456216; EC=2.7.2.3;
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 2/5.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the phosphoglycerate kinase family.
CC       {ECO:0000305}.
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DR   EMBL; X63529; CAA45092.1; -; mRNA.
DR   EMBL; X63528; CAA45091.1; -; Genomic_DNA.
DR   AlphaFoldDB; P50313; -.
DR   SMR; P50313; -.
DR   OMA; DMIFDIG; -.
DR   UniPathway; UPA00109; UER00185.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004618; F:phosphoglycerate kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00318; Phosphoglycerate_kinase; 1.
DR   Gene3D; 3.40.50.1260; -; 3.
DR   HAMAP; MF_00145; Phosphoglyc_kinase; 1.
DR   InterPro; IPR001576; Phosphoglycerate_kinase.
DR   InterPro; IPR015911; Phosphoglycerate_kinase_CS.
DR   InterPro; IPR015824; Phosphoglycerate_kinase_N.
DR   InterPro; IPR036043; Phosphoglycerate_kinase_sf.
DR   PANTHER; PTHR11406; PTHR11406; 1.
DR   Pfam; PF00162; PGK; 1.
DR   PIRSF; PIRSF000724; Pgk; 1.
DR   PRINTS; PR00477; PHGLYCKINASE.
DR   SUPFAM; SSF53748; SSF53748; 1.
DR   PROSITE; PS00111; PGLYCERATE_KINASE; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Glycolysis; Kinase; Nucleotide-binding; Transferase.
FT   CHAIN           1..420
FT                   /note="Phosphoglycerate kinase"
FT                   /id="PRO_0000145861"
FT   BINDING         25..27
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         40
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         64..67
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         123
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         171
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         220
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         313
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         344
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         373..376
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   420 AA;  45914 MW;  FCCE8F842FEA8016 CRC64;
     MLSRKIGIDH ILKHIENKRV LMRVDFNVPL KEGKVKDPTR IQGSVPSIKK ILEQNPKGLV
     LMSHLGRPDG NRVEKHSMKP VVPKLEELLG TKVTFLNDCV GKDVEEAVKS SRNGEIILLE
     NLRFHPEEEG KYIDAAGNKV KADSKAVKEF RKSLTSLGDL FVNDAFGTAH RAHSSMVGVD
     HKIRAAGYLL KRELDYFSKA LESPNRPFLV VLGGAKVKDK IQLIESMLDK VDEMIIGGGM
     AFTFLKKIHN IEIGNSLFDE EGYKIVDQLL EKAKAKNVKI HLPVDFLCGD SLEANANTRS
     FDLKSGIPAG WIGLDAGPKT MAENAEAVAR ANTIVWNGPQ GRFEVDKFKV GSSDLLKHVA
     TRTSQGATSI IGGGDTVNLV QQEKATQKVS HVSTGGGASL ELLEGKVLPG VAYLTDIDQL