PGLR1_ARATH
ID PGLR1_ARATH Reviewed; 422 AA.
AC P49062;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Exopolygalacturonase clone GBGE184;
DE Short=ExoPG;
DE EC=3.2.1.67;
DE AltName: Full=Galacturan 1,4-alpha-galacturonidase;
DE AltName: Full=Pectinase;
DE Flags: Precursor;
GN Name=PGA3; OrderedLocusNames=At1g02790; ORFNames=F22D16.22, T14P4_2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. C24; TISSUE=Flower bud;
RX PubMed=10485285; DOI=10.1007/s004380051042;
RA Torki M., Mandaron P., Thomas F., Quigley F., Mache R., Falconet D.;
RT "Differential expression of a polygalacturonase gene family in Arabidopsis
RT thaliana.";
RL Mol. Gen. Genet. 261:948-952(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Torki M., Thomas F., Mache R., Mandaron P., Falconet D.;
RT "Organisation and expression of a family of polygalacturonase gene in
RT Arabidopsis thaliana.";
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: May function in depolymerizing pectin during pollen
CC development, germination, and tube growth. Acts as an exo-
CC polygalacturonase.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->4)-alpha-D-galacturonosyl](n) + H2O = [(1->4)-alpha-D-
CC galacturonosyl](n-1) + alpha-D-galacturonate; Xref=Rhea:RHEA:14117,
CC Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14572, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:58658, ChEBI:CHEBI:140523; EC=3.2.1.67;
CC -!- SUBCELLULAR LOCATION: Secreted. Secreted, cell wall.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family. {ECO:0000305}.
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DR EMBL; X72291; CAA51032.1; -; mRNA.
DR EMBL; Y16230; CAA76127.1; -; Genomic_DNA.
DR EMBL; AC009525; AAF02888.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27470.1; -; Genomic_DNA.
DR EMBL; AF428425; AAL16194.1; -; mRNA.
DR EMBL; AY065210; AAL38686.1; -; mRNA.
DR EMBL; AY133812; AAM91746.1; -; mRNA.
DR PIR; S34199; S34199.
DR RefSeq; NP_171778.1; NM_100158.3.
DR AlphaFoldDB; P49062; -.
DR SMR; P49062; -.
DR STRING; 3702.AT1G02790.1; -.
DR CAZy; GH28; Glycoside Hydrolase Family 28.
DR PaxDb; P49062; -.
DR PRIDE; P49062; -.
DR ProteomicsDB; 236142; -.
DR EnsemblPlants; AT1G02790.1; AT1G02790.1; AT1G02790.
DR GeneID; 839391; -.
DR Gramene; AT1G02790.1; AT1G02790.1; AT1G02790.
DR KEGG; ath:AT1G02790; -.
DR Araport; AT1G02790; -.
DR TAIR; locus:2024680; AT1G02790.
DR eggNOG; ENOG502QV2R; Eukaryota.
DR HOGENOM; CLU_016031_2_2_1; -.
DR InParanoid; P49062; -.
DR OMA; MAWRSAC; -.
DR OrthoDB; 1028572at2759; -.
DR PhylomeDB; P49062; -.
DR BioCyc; ARA:AT1G02790-MON; -.
DR PRO; PR:P49062; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P49062; baseline and differential.
DR Genevisible; P49062; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0047911; F:galacturan 1,4-alpha-galacturonidase activity; IEA:UniProtKB-EC.
DR GO; GO:0004650; F:polygalacturonase activity; ISS:TAIR.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR Gene3D; 2.160.20.10; -; 1.
DR InterPro; IPR000743; Glyco_hydro_28.
DR InterPro; IPR006626; PbH1.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR Pfam; PF00295; Glyco_hydro_28; 1.
DR SMART; SM00710; PbH1; 5.
DR SUPFAM; SSF51126; SSF51126; 1.
DR PROSITE; PS00502; POLYGALACTURONASE; 1.
PE 2: Evidence at transcript level;
KW Cell wall; Cell wall biogenesis/degradation; Disulfide bond; Glycoprotein;
KW Glycosidase; Hydrolase; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..422
FT /note="Exopolygalacturonase clone GBGE184"
FT /id="PRO_0000024799"
FT REPEAT 200..226
FT /note="PbH1 1"
FT /evidence="ECO:0000255"
FT REPEAT 227..248
FT /note="PbH1 2"
FT /evidence="ECO:0000255"
FT REPEAT 250..270
FT /note="PbH1 3"
FT /evidence="ECO:0000255"
FT REPEAT 280..301
FT /note="PbH1 4"
FT /evidence="ECO:0000255"
FT REPEAT 310..331
FT /note="PbH1 5"
FT /evidence="ECO:0000255"
FT ACT_SITE 241
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:O74213"
FT ACT_SITE 264
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10052"
FT CARBOHYD 229
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 252
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 287
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 243..260
FT /evidence="ECO:0000250|UniProtKB:O74213"
FT DISULFID 366..372
FT /evidence="ECO:0000250|UniProtKB:O74213"
FT DISULFID 404..420
FT /evidence="ECO:0000250|UniProtKB:O74213"
SQ SEQUENCE 422 AA; 44430 MW; 17B02E2CE979B46A CRC64;
MANARSLVAK ANNINVGSLI LMALVFGSCV ANGEYLGGRR GLAANSGNPT VYDITKFGAV
GDGSTNTFKA FLNTWIQVCD SPVPATLLVP KGTFLAGPVI FAGPCKSKVT VNVIGTIIAT
TSGYATPEWF LFERVDNLVL TGTGTFHGKG EAVWKADGCG KKVQCNLPPT SLKFRNMKNV
EINGISSVNA KAFHMFLVKT ENVNIQNIKL TAPAESPNTD GIHLSNADNV SILDSTIATG
DDCVSVGRGS NNVTVERVIC GPGHGLSVGS LGKYKNEEDV SGIHVNNCTM IETDNGLRIK
TWGGSDPSKA VDIKFENIIM QSVKNPIIID QNYGSRGGDS QVAISDILFK NIRGTTITKD
VVQIMCSKSV PCQGVNVVDV NLDYVGKTGG EKKSSSGGLV GALCDNANVI FGGKLSFPMC
PK
