ID   PGLR2_CHAOB             Reviewed;         514 AA.
AC   Q7M1E7;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Polygalacturonase;
DE            Short=PG;
DE            EC=3.2.1.15;
DE   AltName: Full=Major pollen allergen Cha o 2;
DE   AltName: Full=Pectinase;
DE   AltName: Allergen=Cha o 2;
DE   Flags: Precursor;
OS   Chamaecyparis obtusa (Hinoki false-cypress) (Retinospora obtusa).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers II; Cupressales; Cupressaceae;
OC   Chamaecyparis.
OX   NCBI_TaxID=13415;
RN   [1]
RP   NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 51-62.
RC   TISSUE=Pollen;
RX   PubMed=10486272; DOI=10.1006/bbrc.1999.1261;
RA   Mori T., Yokoyama M., Komiyama N., Okano M., Kino K.;
RT   "Purification, identification, and cDNA cloning of Cha o 2, the second
RT   major allergen of Japanese cypress pollen.";
RL   Biochem. Biophys. Res. Commun. 263:166-171(1999).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(1,4-alpha-D-galacturonosyl)n+m + H2O = (1,4-alpha-D-
CC         galacturonosyl)n + (1,4-alpha-D-galacturonosyl)m.; EC=3.2.1.15;
CC   -!- SUBCELLULAR LOCATION: Secreted. Secreted, cell wall.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family. {ECO:0000305}.
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DR   PIR; JC7100; JC7100.
DR   AlphaFoldDB; Q7M1E7; -.
DR   SMR; Q7M1E7; -.
DR   Allergome; 198; Cha o 2.
DR   Allergome; 3187; Cha o 2.0101.
DR   CAZy; GH28; Glycoside Hydrolase Family 28.
DR   PRIDE; Q7M1E7; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004650; F:polygalacturonase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009835; P:fruit ripening; IEA:UniProtKB-KW.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR000743; Glyco_hydro_28.
DR   InterPro; IPR006626; PbH1.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   Pfam; PF00295; Glyco_hydro_28; 1.
DR   SMART; SM00710; PbH1; 5.
DR   SUPFAM; SSF51126; SSF51126; 1.
DR   PROSITE; PS00502; POLYGALACTURONASE; 1.
PE   1: Evidence at protein level;
KW   Allergen; Cell wall; Cell wall biogenesis/degradation;
KW   Direct protein sequencing; Fruit ripening; Glycoprotein; Glycosidase;
KW   Hydrolase; Repeat; Secreted; Signal; Zymogen.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   PROPEP          23..50
FT                   /evidence="ECO:0000269|PubMed:10486272"
FT                   /id="PRO_0000024814"
FT   CHAIN           51..514
FT                   /note="Polygalacturonase"
FT                   /id="PRO_0000024815"
FT   REPEAT          214..240
FT                   /note="PbH1 1"
FT   REPEAT          241..262
FT                   /note="PbH1 2"
FT   REPEAT          264..284
FT                   /note="PbH1 3"
FT   REPEAT          294..315
FT                   /note="PbH1 4"
FT   REPEAT          323..344
FT                   /note="PbH1 5"
FT   REPEAT          357..384
FT                   /note="PbH1 6"
FT   ACT_SITE        255
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10052"
FT   ACT_SITE        278
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10052"
FT   CARBOHYD        266
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        397
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   514 AA;  56073 MW;  68B62DEBDD59C1EC CRC64;
     MGMKFMAAVA FLALQLIVMA AAEDQSAQIM LDSDIEQYLR SNRSLKKLVH SRHDAATVFN
     VEQYGAVGDG KHDSTEAFAT TWNAACKKAS AVLLVPANKK FFVNNLVFRG PCQPHLSFKV
     DGTIVAQPDP ARWKNSKIWL QFAQLTDFNL MGTGVIDGQG QQWWAGQCKV VNGRTVCNDR
     NRPTAIKIDY SKSVTVKELT LMNSPEFHLV FGECEGVKIQ GLKIKAPRDS PNTDGIDIFA
     SKRFHIEKCV IGTGDDCIAI GTGSSNITIK DLICGPGHGI SIGSLGRDNS RAEVSHVHVN
     RAKFIDTQNG LRIKTWQGGS GLASYITYEN VEMINSENPI LINQFYCTSA SACQNQRSAV
     QIQGVTYKNI HGTSATAAAI QLMCSDSVPC TGIQLSNVSL KLTSGKPASC VDKNARGFYS
     GRLIPTCKNL RPGPSPKEFE LQQQPTTVMD ENKGACAKGD STCISLSSSP PNCKNKCKGC
     QPCKPKLIIV HPNKPQDYYP QKWVCSCHNK IYNP