ID   PGLR3_ARATH             Reviewed;         456 AA.
AC   Q9LW07;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 135.
DE   RecName: Full=Probable polygalacturonase At3g15720;
DE            Short=PG;
DE            EC=3.2.1.15;
DE   AltName: Full=Pectinase At3g15720;
DE   Flags: Precursor;
GN   OrderedLocusNames=At3g15720; ORFNames=MSJ11.12;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(1,4-alpha-D-galacturonosyl)n+m + H2O = (1,4-alpha-D-
CC         galacturonosyl)n + (1,4-alpha-D-galacturonosyl)m.; EC=3.2.1.15;
CC   -!- SUBCELLULAR LOCATION: Secreted, cell wall.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=1;
CC         Comment=A number of isoforms are produced. According to EST
CC         sequences.;
CC       Name=1;
CC         IsoId=Q9LW07-1; Sequence=Displayed;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family. {ECO:0000305}.
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DR   EMBL; AB017071; BAB02303.1; -; Genomic_DNA.
DR   EMBL; CP002686; AEE75718.1; -; Genomic_DNA.
DR   RefSeq; NP_566524.1; NM_112442.2. [Q9LW07-1]
DR   AlphaFoldDB; Q9LW07; -.
DR   SMR; Q9LW07; -.
DR   STRING; 3702.AT3G15720.1; -.
DR   CAZy; GH28; Glycoside Hydrolase Family 28.
DR   PaxDb; Q9LW07; -.
DR   ProteomicsDB; 236151; -. [Q9LW07-1]
DR   EnsemblPlants; AT3G15720.1; AT3G15720.1; AT3G15720. [Q9LW07-1]
DR   GeneID; 820815; -.
DR   Gramene; AT3G15720.1; AT3G15720.1; AT3G15720. [Q9LW07-1]
DR   KEGG; ath:AT3G15720; -.
DR   Araport; AT3G15720; -.
DR   TAIR; locus:2093212; AT3G15720.
DR   eggNOG; ENOG502QST2; Eukaryota.
DR   HOGENOM; CLU_016031_2_2_1; -.
DR   InParanoid; Q9LW07; -.
DR   OrthoDB; 1028572at2759; -.
DR   PhylomeDB; Q9LW07; -.
DR   BioCyc; ARA:AT3G15720-MON; -.
DR   PRO; PR:Q9LW07; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q9LW07; baseline and differential.
DR   Genevisible; Q9LW07; AT.
DR   GO; GO:0031225; C:anchored component of membrane; TAS:TAIR.
DR   GO; GO:0046658; C:anchored component of plasma membrane; HDA:TAIR.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR   GO; GO:0009505; C:plant-type cell wall; HDA:TAIR.
DR   GO; GO:0004650; F:polygalacturonase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR000743; Glyco_hydro_28.
DR   InterPro; IPR006626; PbH1.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   Pfam; PF00295; Glyco_hydro_28; 1.
DR   SMART; SM00710; PbH1; 6.
DR   SUPFAM; SSF51126; SSF51126; 1.
DR   PROSITE; PS00502; POLYGALACTURONASE; 1.
PE   3: Inferred from homology;
KW   Alternative splicing; Cell wall; Cell wall biogenesis/degradation;
KW   Glycosidase; Hydrolase; Reference proteome; Repeat; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..456
FT                   /note="Probable polygalacturonase At3g15720"
FT                   /id="PRO_0000259446"
FT   REPEAT          169..195
FT                   /note="PbH1 1"
FT   REPEAT          196..217
FT                   /note="PbH1 2"
FT   REPEAT          219..239
FT                   /note="PbH1 3"
FT   REPEAT          249..270
FT                   /note="PbH1 4"
FT   REPEAT          278..299
FT                   /note="PbH1 5"
FT   REPEAT          314..341
FT                   /note="PbH1 6"
FT   ACT_SITE        210
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10052"
FT   ACT_SITE        233
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10052"
SQ   SEQUENCE   456 AA;  49300 MW;  E85AFE25CACD5CBD CRC64;
     MKKKTWFLNF SLFFLQIFTS SNALDVTQFG AVGDGVTDDS QAFLKAWEAV CSGTGDGQFV
     VPAGMTFMLQ PLKFQGSCKS TPVFVQMLGK LVAPSKGNWK GDKDQWILFT DIEGLVIEGD
     GEINGQGSSW WEHKGSRPTA LKFRSCNNLR LSGLTHLDSP MAHIHISECN YVTISSLRIN
     APESSPNTDG IDVGASSNVV IQDCIIATGD DCIAINSGTS NIHISGIDCG PGHGISIGSL
     GKDGETATVE NVCVQNCNFR GTMNGARIKT WQGGSGYARM ITFNGITLDN VENPIIIDQF
     YNGGDSDNAK DRKSSAVEVS KVVFSNFIGT SKSEYGVDFR CSERVPCTEI FLRDMKIETA
     SSGSGQVAQG QCLNVRGAST IAVPGLECLE LSTDMFSSAQ LLEQTCMSAQ SVQPRTTTQP
     MQDPIWVFQS RGKQLRVYNI AILVSFISLV TYILAR