PGLR5_ARATH
ID PGLR5_ARATH Reviewed; 444 AA.
AC Q94AJ5; Q9SSC2;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Probable polygalacturonase At1g80170;
DE Short=PG;
DE EC=3.2.1.15;
DE AltName: Full=Pectinase At1g80170;
DE Flags: Precursor;
GN OrderedLocusNames=At1g80170; ORFNames=F18B13.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=17010199; DOI=10.1186/gb-2006-7-9-r87;
RA Kim J., Shiu S.-H., Thoma S., Li W.-H., Patterson S.E.;
RT "Patterns of expansion and expression divergence in the plant
RT polygalacturonase gene family.";
RL Genome Biol. 7:R87.1-R87.14(2006).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17928369; DOI=10.1093/jxb/erm222;
RA Gonzalez-Carranza Z.H., Elliott K.A., Roberts J.A.;
RT "Expression of polygalacturonases and evidence to support their role during
RT cell separation processes in Arabidopsis thaliana.";
RL J. Exp. Bot. 58:3719-3730(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(1,4-alpha-D-galacturonosyl)n+m + H2O = (1,4-alpha-D-
CC galacturonosyl)n + (1,4-alpha-D-galacturonosyl)m.; EC=3.2.1.15;
CC -!- INTERACTION:
CC Q94AJ5; Q8VZW1: NIP1-1; NbExp=2; IntAct=EBI-4457426, EBI-4424378;
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in young, mature and dehiscing anthers.
CC Found in stems, but not in roots or in abscission zone of floral
CC organs. {ECO:0000269|PubMed:17010199, ECO:0000269|PubMed:17928369}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD55489.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC009322; AAD55489.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE36366.1; -; Genomic_DNA.
DR EMBL; AY046002; AAK76676.1; -; mRNA.
DR EMBL; AY142668; AAN13206.1; -; mRNA.
DR PIR; D96833; D96833.
DR RefSeq; NP_565232.1; NM_106666.4.
DR AlphaFoldDB; Q94AJ5; -.
DR SMR; Q94AJ5; -.
DR BioGRID; 29575; 2.
DR IntAct; Q94AJ5; 2.
DR STRING; 3702.AT1G80170.1; -.
DR CAZy; GH28; Glycoside Hydrolase Family 28.
DR iPTMnet; Q94AJ5; -.
DR PaxDb; Q94AJ5; -.
DR PRIDE; Q94AJ5; -.
DR ProteomicsDB; 236406; -.
DR EnsemblPlants; AT1G80170.1; AT1G80170.1; AT1G80170.
DR GeneID; 844357; -.
DR Gramene; AT1G80170.1; AT1G80170.1; AT1G80170.
DR KEGG; ath:AT1G80170; -.
DR Araport; AT1G80170; -.
DR TAIR; locus:2016314; AT1G80170.
DR eggNOG; ENOG502QRJW; Eukaryota.
DR HOGENOM; CLU_016031_2_3_1; -.
DR InParanoid; Q94AJ5; -.
DR OMA; FHKCKNL; -.
DR OrthoDB; 1028572at2759; -.
DR PhylomeDB; Q94AJ5; -.
DR BioCyc; ARA:AT1G80170-MON; -.
DR PRO; PR:Q94AJ5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q94AJ5; baseline and differential.
DR Genevisible; Q94AJ5; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0004650; F:polygalacturonase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR Gene3D; 2.160.20.10; -; 1.
DR InterPro; IPR000743; Glyco_hydro_28.
DR InterPro; IPR006626; PbH1.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR Pfam; PF00295; Glyco_hydro_28; 1.
DR SMART; SM00710; PbH1; 6.
DR SUPFAM; SSF51126; SSF51126; 1.
DR PROSITE; PS00502; POLYGALACTURONASE; 1.
PE 1: Evidence at protein level;
KW Cell wall; Cell wall biogenesis/degradation; Glycosidase; Hydrolase;
KW Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT CHAIN 29..444
FT /note="Probable polygalacturonase At1g80170"
FT /id="PRO_0000367917"
FT REPEAT 208..234
FT /note="PbH1 1"
FT REPEAT 235..256
FT /note="PbH1 2"
FT REPEAT 258..278
FT /note="PbH1 3"
FT REPEAT 288..309
FT /note="PbH1 4"
FT REPEAT 317..338
FT /note="PbH1 5"
FT REPEAT 351..378
FT /note="PbH1 6"
FT ACT_SITE 249
FT /note="Proton donor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10052"
FT ACT_SITE 272
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10052"
SQ SEQUENCE 444 AA; 48172 MW; 8FC52117E96B6418 CRC64;
MSYSRGGTLV TLLLLLVVAS SLALTANANS FESLLQLPRR QSRSTRPRSE RLLHVGNFGA
KGNGVTDDTK AFADAWKTAC SSKVKTRILV PENYTCLLRP IDLSGPCKAR LTLQISGTII
APNDPDVWEG LNRRKWLYFH GLSRLTVEGG GTVNGMGQEW WERSCKHNHS NPCRGAPTAL
TFHKCKNMRV ENLNVIDSQQ MHIALTSCRR VTISGLKVIA PATSPNTDGI HISVSRGIVI
DNTTVSTGDD CISIVKNSTQ ISISNIICGP GHGISIGSLG KSKSWEEVRD ITVDTAIISD
TANGVRIKTW QGGSGLVSKI IFRNIKMNNV SNPIIIDQYY CDSRKPCANQ TSAISIENIS
FVHVRGTSAS KEAIKISCSD SSPCRNILLQ DIDLEPSNGD GFTESFCWEA YGSSSGQVYP
PPCLSDDTSF LEQSVQSGIT SAYL
