PGLR6_ARATH
ID PGLR6_ARATH Reviewed; 405 AA.
AC O22818;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Probable polygalacturonase At2g43860;
DE Short=PG;
DE EC=3.2.1.15;
DE AltName: Full=Pectinase At2g43860;
DE Flags: Precursor;
GN OrderedLocusNames=At2g43860; ORFNames=F18O19.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=17010199; DOI=10.1186/gb-2006-7-9-r87;
RA Kim J., Shiu S.-H., Thoma S., Li W.-H., Patterson S.E.;
RT "Patterns of expansion and expression divergence in the plant
RT polygalacturonase gene family.";
RL Genome Biol. 7:R87.1-R87.14(2006).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17928369; DOI=10.1093/jxb/erm222;
RA Gonzalez-Carranza Z.H., Elliott K.A., Roberts J.A.;
RT "Expression of polygalacturonases and evidence to support their role during
RT cell separation processes in Arabidopsis thaliana.";
RL J. Exp. Bot. 58:3719-3730(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(1,4-alpha-D-galacturonosyl)n+m + H2O = (1,4-alpha-D-
CC galacturonosyl)n + (1,4-alpha-D-galacturonosyl)m.; EC=3.2.1.15;
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in developing and germinated seeds in the
CC chalazal region immediately adjacent to the site of penetration of the
CC radicle through the testa. {ECO:0000269|PubMed:17010199,
CC ECO:0000269|PubMed:17928369}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family. {ECO:0000305}.
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DR EMBL; AC002333; AAB64020.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC10336.1; -; Genomic_DNA.
DR EMBL; DQ056577; AAY78726.1; -; mRNA.
DR PIR; D84871; D84871.
DR RefSeq; NP_181914.1; NM_129948.2.
DR AlphaFoldDB; O22818; -.
DR SMR; O22818; -.
DR STRING; 3702.AT2G43860.1; -.
DR CAZy; GH28; Glycoside Hydrolase Family 28.
DR PaxDb; O22818; -.
DR PRIDE; O22818; -.
DR ProteomicsDB; 236431; -.
DR EnsemblPlants; AT2G43860.1; AT2G43860.1; AT2G43860.
DR GeneID; 818990; -.
DR Gramene; AT2G43860.1; AT2G43860.1; AT2G43860.
DR KEGG; ath:AT2G43860; -.
DR Araport; AT2G43860; -.
DR TAIR; locus:2043974; AT2G43860.
DR eggNOG; ENOG502QRN7; Eukaryota.
DR HOGENOM; CLU_016031_2_2_1; -.
DR InParanoid; O22818; -.
DR OMA; WDVACAS; -.
DR OrthoDB; 1028572at2759; -.
DR PhylomeDB; O22818; -.
DR BioCyc; ARA:AT2G43860-MON; -.
DR PRO; PR:O22818; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22818; baseline and differential.
DR Genevisible; O22818; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0004650; F:polygalacturonase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR Gene3D; 2.160.20.10; -; 1.
DR InterPro; IPR000743; Glyco_hydro_28.
DR InterPro; IPR006626; PbH1.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR Pfam; PF00295; Glyco_hydro_28; 1.
DR SMART; SM00710; PbH1; 3.
DR SUPFAM; SSF51126; SSF51126; 1.
PE 2: Evidence at transcript level;
KW Cell wall; Cell wall biogenesis/degradation; Glycosidase; Hydrolase;
KW Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..405
FT /note="Probable polygalacturonase At2g43860"
FT /id="PRO_0000367918"
FT REPEAT 184..210
FT /note="PbH1 1"
FT REPEAT 211..232
FT /note="PbH1 2"
FT REPEAT 264..285
FT /note="PbH1 3"
FT ACT_SITE 225
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 248
FT /evidence="ECO:0000250"
SQ SEQUENCE 405 AA; 43462 MW; 556D65570A44D195 CRC64;
MVYNTSCLIL PLALIILDFT LISSLAHPIP STLNVLSYGA KPDGSKDSTK AFLAAWDVAC
ASANPTTIIV PKGRFLVGNL VFHGNECKQA PISIRIAGSI VAPEDFRIIA SSKHWIWFED
VTDVSIYGGI LDAQGTSLWK CKNNGGHNCP TGAKSLVFSG SNNIKISGLT SINSQKFHIV
IDNSNNVNID GVKVSADENS PNTDGIHVES SHSVHITNSR IGTGDDCISI GPGSTNVFIQ
TIRCGPGHGI SIGSLGRAEE EQGVDNVTVS NVDFMGTNNG VRIKTWGKDS NSFARNIVFQ
HINMKMVKNP IIIDQHYCLH KPCPKQESGV KVSNVRYEDI HGTSNTEVAV LLDCSKEKPC
TGIVMDDVNL VSVHRPAQAS CDNANGSAND VVPFTPCLKR EIIIT
