PGLR_CUPSE
ID PGLR_CUPSE Reviewed; 164 AA.
AC C0HKB1;
DT 15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2017, sequence version 1.
DT 03-AUG-2022, entry version 12.
DE RecName: Full=Polygalacturonase {ECO:0000303|PubMed:28439939};
DE EC=3.2.1.15 {ECO:0000250|UniProtKB:P05117};
DE AltName: Allergen=Cup s 2 {ECO:0000303|PubMed:28439939};
DE Flags: Fragments;
OS Cupressus sempervirens (Italian cypress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Pinopsida; Pinidae; Conifers II; Cupressales; Cupressaceae;
OC Cupressus.
OX NCBI_TaxID=13469 {ECO:0000303|PubMed:22287175};
RN [1] {ECO:0000305}
RP PROTEIN SEQUENCE OF 1-10, ALLERGEN, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC TISSUE=Pollen {ECO:0000303|PubMed:28439939};
RX PubMed=28439939; DOI=10.1111/all.13191;
RA Shahali Y., Sutra J.P., Hilger C., Swiontek K., Haddad I., Vinh J.,
RA Guilloux L., Charpin D., Senechal H., Poncet P.;
RT "Identification of a polygalacturonase (Cup s 2) as the major CCD-bearing
RT allergen in Cupressus sempervirens pollen.";
RL Allergy 72:1806-1810(2017).
RN [2] {ECO:0000305}
RP PROTEIN SEQUENCE OF 11-164, ALLERGEN, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC TISSUE=Pollen {ECO:0000303|PubMed:22287175};
RX PubMed=22287175; DOI=10.1002/elps.201100324;
RA Shahali Y., Sutra J.P., Haddad I., Vinh J., Guilloux L., Peltre G.,
RA Senechal H., Poncet P.;
RT "Proteomics of cypress pollen allergens using double and triple one-
RT dimensional electrophoresis.";
RL Electrophoresis 33:462-469(2012).
RN [3] {ECO:0000305}
RP ALLERGEN, MASS SPECTROMETRY, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Pollen {ECO:0000303|PubMed:22813879};
RX PubMed=22813879; DOI=10.1016/j.jprot.2012.07.010;
RA Shahali Y., Sutra J.P., Fasoli E., D'Amato A., Righetti P.G., Futamura N.,
RA Boschetti E., Senechal H., Poncet P.;
RT "Allergomic study of cypress pollen via combinatorial peptide ligand
RT libraries.";
RL J. Proteomics 77:101-110(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(1,4-alpha-D-galacturonosyl)n+m + H2O = (1,4-alpha-D-
CC galacturonosyl)n + (1,4-alpha-D-galacturonosyl)m.; EC=3.2.1.15;
CC Evidence={ECO:0000250|UniProtKB:P05117};
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000250|UniProtKB:P05117}.
CC -!- MASS SPECTROMETRY: Mass=56835; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:22813879};
CC -!- ALLERGEN: Causes an allergenic reaction in human. Binds to IgE.
CC {ECO:0000269|PubMed:22287175, ECO:0000269|PubMed:22813879,
CC ECO:0000269|PubMed:28439939}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family. {ECO:0000305}.
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DR AlphaFoldDB; C0HKB1; -.
DR Allergome; 10177; Cup s 2.
DR Allergome; 11995; Cup s 2.0101.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0004650; F:polygalacturonase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR Gene3D; 2.160.20.10; -; 2.
DR InterPro; IPR000743; Glyco_hydro_28.
DR InterPro; IPR012334; Pectin_lyas_fold.
DR InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR Pfam; PF00295; Glyco_hydro_28; 1.
DR SUPFAM; SSF51126; SSF51126; 1.
DR PROSITE; PS00502; POLYGALACTURONASE; 1.
PE 1: Evidence at protein level;
KW Allergen; Cell wall; Cell wall biogenesis/degradation;
KW Direct protein sequencing; Glycosidase; Hydrolase; Secreted.
FT CHAIN <1..>164
FT /note="Polygalacturonase"
FT /evidence="ECO:0000305"
FT /id="PRO_0000438848"
FT NON_CONS 10..11
FT /evidence="ECO:0000303|PubMed:22287175"
FT NON_CONS 23..24
FT /evidence="ECO:0000303|PubMed:22287175"
FT NON_CONS 36..37
FT /evidence="ECO:0000303|PubMed:22287175"
FT NON_CONS 43..44
FT /evidence="ECO:0000303|PubMed:22287175"
FT NON_CONS 62..63
FT /evidence="ECO:0000303|PubMed:22287175"
FT NON_CONS 90..91
FT /evidence="ECO:0000303|PubMed:22287175"
FT NON_CONS 96..97
FT /evidence="ECO:0000303|PubMed:22287175"
FT NON_CONS 138..139
FT /evidence="ECO:0000303|PubMed:22287175"
FT NON_CONS 149..150
FT /evidence="ECO:0000303|PubMed:22287175"
FT NON_TER 1
FT /evidence="ECO:0000303|PubMed:22287175"
FT NON_TER 164
FT /evidence="ECO:0000303|PubMed:22287175"
SQ SEQUENCE 164 AA; 17996 MW; 477646F12FD615DA CRC64;
DVAIVFNVEH TLSAVFLVPA NKKVDGIIAA YPDPVKIWMH FARTVCNDKG RPTAIKIDFS
KSELTLMNSP EFHLVFGECD GVKIQGIKIK RFEIEKDLTC GPGHGMSIGS LGKGNSRSEV
SFVHLDGAKF IDTQNGLRSA VKIEDVTFKN ANGYYTNPLN PPCK
