ID   PGLR_RALSO              Reviewed;         531 AA.
AC   P58598;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2002, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Polygalacturonase;
DE            Short=PGA;
DE            EC=3.2.1.15;
DE   AltName: Full=Pectinase;
DE   Flags: Precursor;
GN   Name=pglA; OrderedLocusNames=RSp0880; ORFNames=RS01651;
OS   Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OG   Plasmid megaplasmid Rsp.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=267608;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GMI1000;
RX   PubMed=11823852; DOI=10.1038/415497a;
RA   Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA   Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA   Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA   Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA   Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT   "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL   Nature 415:497-502(2002).
CC   -!- FUNCTION: Contributes to the wilt disease production on tomato.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(1,4-alpha-D-galacturonosyl)n+m + H2O = (1,4-alpha-D-
CC         galacturonosyl)n + (1,4-alpha-D-galacturonosyl)m.; EC=3.2.1.15;
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family. {ECO:0000305}.
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DR   EMBL; AL646053; CAD18031.1; -; Genomic_DNA.
DR   RefSeq; WP_011004177.1; NC_003296.1.
DR   AlphaFoldDB; P58598; -.
DR   SMR; P58598; -.
DR   STRING; 267608.RSp0880; -.
DR   CAZy; GH28; Glycoside Hydrolase Family 28.
DR   EnsemblBacteria; CAD18031; CAD18031; RSp0880.
DR   GeneID; 60503788; -.
DR   KEGG; rso:RSp0880; -.
DR   PATRIC; fig|267608.8.peg.4350; -.
DR   eggNOG; COG5434; Bacteria.
DR   HOGENOM; CLU_016031_0_0_4; -.
DR   OMA; HFYYGHG; -.
DR   Proteomes; UP000001436; Plasmid megaplasmid Rsp.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004650; F:polygalacturonase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR000743; Glyco_hydro_28.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   Pfam; PF00295; Glyco_hydro_28; 1.
DR   SUPFAM; SSF51126; SSF51126; 1.
DR   PROSITE; PS00502; POLYGALACTURONASE; 1.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Glycosidase; Hydrolase; Plasmid;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..531
FT                   /note="Polygalacturonase"
FT                   /id="PRO_0000024760"
FT   REGION          518..531
FT                   /note="Required for PGA export across the outer membrane
FT                   and catalytic activity"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        307
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10052"
FT   ACT_SITE        333
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10052"
SQ   SEQUENCE   531 AA;  54773 MW;  E6B8628F3890E281 CRC64;
     MILSHRYTLI ALAAAILSSG AHAAGASVTA SWGNVAEPSL PADSAVCKTL AATITPVSGS
     IDTVDGNPSN SQPDASRIQT AIDNCPAGQA VRLVKGSAGE SGFLSGSLKL KSGVTLWIDT
     GVTLFASRNP ADFDNGVGTC GTATTSNTKS CNALIVARDT VGSGIVGDGI IDGRGGSLVT
     SGPNANRLTW WDIAYLNKTK GLNQQNPRLV QLYNGSAFTL YRVTVQNSPN FHIVTTGTAG
     VTAWGIKIVT PSLAYTVPGY KCAAGTTPDK VTPATCFTPE TVKNTDGFDP GQSTNVVLAN
     SYISTGDDHV AIKASGGATR NLLFAHNHFY YGHGLSIGSE TDGGVSNMQV TDLAMDGNDS
     SGGNGLRIKS DISRGGKVNN IVYNGICMRN VKEPLVFDPF YSTAKGSLYP NFTNIVVKNF
     HDLGSAKGIA RTMTFLGYEA KKRTNPLTLT LDNVVFDGTQ PAFDVAHNGG PASPNGTHFT
     FGGNGPVSFA NAIVTSSTTD VTVTGTPGTA AAVDCSNAFV PLKSVAPTSP I