ID   PGLR_VITVI              Reviewed;         491 AA.
AC   A7PZL3;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=Probable polygalacturonase;
DE            Short=PG {ECO:0000250|UniProtKB:Q7M1E7};
DE            EC=3.2.1.15;
DE   AltName: Full=Pectinase {ECO:0000250|UniProtKB:Q7M1E7};
GN   ORFNames=GSVIVT00026920001, LOC100243180;
OS   Vitis vinifera (Grape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; Vitales; Vitaceae; Viteae; Vitis.
OX   NCBI_TaxID=29760;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Pinot noir / PN40024 {ECO:0000269|PubMed:17721507};
RX   PubMed=17721507; DOI=10.1038/nature06148;
RA   Jaillon O., Aury J.-M., Noel B., Policriti A., Clepet C., Casagrande A.,
RA   Choisne N., Aubourg S., Vitulo N., Jubin C., Vezzi A., Legeai F.,
RA   Hugueney P., Dasilva C., Horner D., Mica E., Jublot D., Poulain J.,
RA   Bruyere C., Billault A., Segurens B., Gouyvenoux M., Ugarte E.,
RA   Cattonaro F., Anthouard V., Vico V., Del Fabbro C., Alaux M.,
RA   Di Gaspero G., Dumas V., Felice N., Paillard S., Juman I., Moroldo M.,
RA   Scalabrin S., Canaguier A., Le Clainche I., Malacrida G., Durand E.,
RA   Pesole G., Laucou V., Chatelet P., Merdinoglu D., Delledonne M.,
RA   Pezzotti M., Lecharny A., Scarpelli C., Artiguenave F., Pe M.E., Valle G.,
RA   Morgante M., Caboche M., Adam-Blondon A.-F., Weissenbach J., Quetier F.,
RA   Wincker P.;
RT   "The grapevine genome sequence suggests ancestral hexaploidization in major
RT   angiosperm phyla.";
RL   Nature 449:463-467(2007).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 324-337.
RA   Almagro L., Belchi-Navarro S., Pedreno M.A.;
RL   Submitted (JUL-2008) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(1,4-alpha-D-galacturonosyl)n+m + H2O = (1,4-alpha-D-
CC         galacturonosyl)n + (1,4-alpha-D-galacturonosyl)m.; EC=3.2.1.15;
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family. {ECO:0000255}.
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DR   AlphaFoldDB; A7PZL3; -.
DR   SMR; A7PZL3; -.
DR   STRING; 29760.VIT_15s0046g02000.t01; -.
DR   PRIDE; A7PZL3; -.
DR   EnsemblPlants; Vitvi15g04607_t001; Vitvi15g04607_P001; Vitvi15g04607.
DR   Gramene; Vitvi15g04607_t001; Vitvi15g04607_P001; Vitvi15g04607.
DR   eggNOG; ENOG502QPMF; Eukaryota.
DR   ExpressionAtlas; A7PZL3; baseline and differential.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004650; F:polygalacturonase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR000743; Glyco_hydro_28.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   Pfam; PF00295; Glyco_hydro_28; 1.
DR   SUPFAM; SSF51126; SSF51126; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Glycoprotein; Glycosidase; Hydrolase; Membrane;
KW   Repeat; Transmembrane; Transmembrane helix.
FT   CHAIN           1..491
FT                   /note="Probable polygalacturonase"
FT                   /id="PRO_0000363726"
FT   TRANSMEM        15..35
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REPEAT          230..256
FT                   /note="PbH1 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          257..278
FT                   /note="PbH1 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          319..340
FT                   /note="PbH1 3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          348..369
FT                   /note="PbH1 4"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        271
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:Q7M1E7"
FT   CARBOHYD        165
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        175
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        214
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        399
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        421
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   491 AA;  53489 MW;  BFEB4EC7843D2BB6 CRC64;
     MVETHKKKIA NLTQPIVSFY CFQVVSVLVA VVLLLSVSRG ECRKGRILEA LEYSAISCRA
     HSASLVDFGG VGDGQTLNTK AFQDAVSELS KYGSEGGAQL YVPAGKWLTG SFSLTSHFTL
     FLHRDAVLLA SQDISQWPVI KPLPSYGRGR DAAAGRYTSL IFGTNLTDVI ITGDNGTIDG
     QGGLWWQRFH GGKLKYTRPY LIELMYSADI QISNLTLLNS PSWNVHPVYS RNILIQGITI
     LAPVRSPNTD GINPDSCTNT RIEDCYIVSG DDCVAVKSGW DEYGIAYGMP TKQLVIRRLT
     CISPYSAVIA LGSEMSGGIQ DVRAEDIVAI NSESGIRIKT GIGRGGYVKD IYVRGMTMKT
     MKWAFWMTGN YGSHADNHYD PKAFPVIQGI NYRDMVAENV SMAARLEGIP SDPFTGICIS
     NVTIHLAAKA KKVPWTCTDV EGISSGVTPT PCSTLPDQGP EKTSLCNFPA ESLPIDTVEL
     QKCSYGINYY P