ID   PGLZ_ECOHS              Reviewed;         865 AA.
AC   P0DUG0; A0A7M3S2P5;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   02-JUN-2021, sequence version 1.
DT   03-AUG-2022, entry version 5.
DE   RecName: Full=Alkaline phosphatase-like protein BrxZ {ECO:0000303|PubMed:30418590};
DE   AltName: Full=BREX protein PglZ {ECO:0000305};
GN   Name=pglZ; Synonyms=brxZ {ECO:0000303|PubMed:30418590};
GN   OrderedLocusNames=EcHS_A0340;
OS   Escherichia coli O9:H4 (strain HS).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=331112;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HS;
RX   PubMed=18676672; DOI=10.1128/jb.00619-08;
RA   Rasko D.A., Rosovitz M.J., Myers G.S.A., Mongodin E.F., Fricke W.F.,
RA   Gajer P., Crabtree J., Sebaihia M., Thomson N.R., Chaudhuri R.,
RA   Henderson I.R., Sperandio V., Ravel J.;
RT   "The pangenome structure of Escherichia coli: comparative genomic analysis
RT   of E. coli commensal and pathogenic isolates.";
RL   J. Bacteriol. 190:6881-6893(2008).
RN   [2]
RP   FUNCTION IN ANTIVIRAL DEFENSE, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=HS;
RX   PubMed=30418590; DOI=10.1093/nar/gky1125;
RA   Gordeeva J., Morozova N., Sierro N., Isaev A., Sinkunas T., Tsvetkova K.,
RA   Matlashov M., Truncaite L., Morgan R.D., Ivanov N.V., Siksnys V., Zeng L.,
RA   Severinov K.;
RT   "BREX system of Escherichia coli distinguishes self from non-self by
RT   methylation of a specific DNA site.";
RL   Nucleic Acids Res. 47:253-265(2019).
CC   -!- FUNCTION: BREX systems (bacteriophage exclusion) provide immunity
CC       against bacteriophage. Part of a type 1 BREX system which protects
CC       against dsDNA phage. This system allows phage adsorption but prevents
CC       phage DNA replication, without degradation of the phage DNA.
CC       Methylation of bacterial DNA by PglX guides self/non-self
CC       discrimination. When the brxA-brxB-brxC-pglX-pglZ-brxL genes are
CC       transformed into a susceptible E.coli strain (BW25113) they confer very
CC       high resistance to infection by bacteriophage VR7 and VpaE1, about 100-
CC       fold protection against lambda, T5 and T7 and no protection against RNA
CC       phage Qbeta, ssDNA phage M13 or dSDNA phage T4 and VR5. Glycosylated
CC       phage DNA is not susceptible to BREX. The BREX system does not confer
CC       resistance to lysogenic lambda phage, i.e. prophage that are integrated
CC       into the chromosomal DNA and then induced to form phage.
CC       {ECO:0000269|PubMed:30418590}.
CC   -!- INDUCTION: Transcribed at similar levels in lag and exponential phase,
CC       rises in stationary phase. {ECO:0000269|PubMed:30418590}.
CC   -!- DISRUPTION PHENOTYPE: No methylation of 5'-GGTAAG-3' in chromosomal
CC       DNA, BREX no longer confers phage resistance.
CC       {ECO:0000269|PubMed:30418590}.
CC   -!- SIMILARITY: Belongs to the alkaline phosphatase superfamily.
CC       {ECO:0000305}.
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DR   EMBL; CP000802; ABV04728.1; -; Genomic_DNA.
DR   RefSeq; WP_001180887.1; NC_009800.1.
DR   AlphaFoldDB; P0DUG0; -.
DR   KEGG; ecx:EcHS_A0340; -.
DR   OMA; DHGFLYQ; -.
DR   Proteomes; UP000001123; Chromosome.
DR   GO; GO:0110165; C:cellular anatomical entity; IEA:UniProt.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR   InterPro; IPR014060; PglZ.
DR   InterPro; IPR013973; PglZ_dom.
DR   Pfam; PF08665; PglZ; 1.
DR   SUPFAM; SSF53649; SSF53649; 1.
DR   TIGRFAMs; TIGR02687; TIGR02687; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense.
FT   CHAIN           1..865
FT                   /note="Alkaline phosphatase-like protein BrxZ"
FT                   /id="PRO_0000452167"
SQ   SEQUENCE   865 AA;  100255 MW;  E347A06D14B50C51 CRC64;
     MQNQDFIAGL KAKFAEHRIV FWHDPDKRFI EELEQLKLEN VTLINMTHAS QLAVKKRIEM
     DEPEQQFLLW FPHDAPPHEQ DWLLDIRLYS SEFHADFAAI TLNTLGIPQL GLREHIQRRK
     AFFSTKRTQA LKNLVTEQED ETSLDKKMIA VIAGAKTAKT EDILFNLITQ YVNQQTEDDS
     ELENTQAMLK RHGLDPVLWE MLNHEMGYQA EEPSLENLLL KLFCTDLSAQ ADRQQRAWLE
     KNVLLTPSGR ASALAFMVTW RADRRYKEAY DYCAQQMQAA LRPEDHYRLS SPYDLHECET
     TLSIEQTIIH ALVTQLLEES TTLDREAFKK LLSERQSKYW CQTQPEYYAI YDALRQAERL
     LNLRNRHIDG FHYQDSATFW KAYCEELFRF DQAYRLFNEY ALLVHSKGAM ILKSLDDYIE
     ALYSNWYLAE LSRNWNEVLE AENRMQAWQI PGVPRQKNFF NEVVKPQFQN PQIKRVFVII
     SDALRYEVAE ELGNQINTEK RFTAELRSQL GVLPSYTQLG MAALLPHEQL CYQPSNGDIV
     YADGLSTSGI PNRDTILKNY KGMAIKSKDL LELKNQEGRD LIRDYEVVYI WHNTIDATGD
     TASTEDKTFE ACRTAVTELK DLVTKVINRL HGTRIFVTAD HGFLFQQQAL SVQDKTTLQI
     KPENTIKNHK RFIIGHQLPA DDFCWKGKVA DTAGVSDNSE FLIPKGIQRF HFSGGARFVH
     GGTMLQEVCV PVLQIKALQK TAAEKQPQRR PVDIVAYHPM IKLVNNIDKV SLLQTHPVGE
     LYEPRILNIY IVDNANNVVS GKERISFDSD NSTMEKRVRE VTLKLIGANF NRRNEYWLIL
     EDAQTETGYQ KYPVIIDLAF QDDFF