PGPLD_DROME
ID PGPLD_DROME Reviewed; 282 AA.
AC Q9GN97; A4V1I9;
DT 10-MAY-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Peptidoglycan-recognition protein LD;
GN Name=PGRP-LD; ORFNames=CG33717;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=11106397; DOI=10.1073/pnas.97.25.13772;
RA Werner T., Liu G., Kang D., Ekengren S., Steiner H., Hultmark D.;
RT "A family of peptidoglycan recognition proteins in the fruit fly Drosophila
RT melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:13772-13777(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Peptidoglycan-recognition protein probably involved in innate
CC immunity by binding to peptidoglycans (PGN) of bacteria and activating
CC the immune response. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in uninduced hemocytes and mbn-2 cells.
CC {ECO:0000269|PubMed:11106397}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in 0-5 hours embryos and in adult
CC females, suggesting that it is expressed maternally.
CC {ECO:0000269|PubMed:11106397}.
CC -!- SIMILARITY: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.
CC {ECO:0000305}.
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DR EMBL; AF313389; AAG32062.1; -; mRNA.
DR EMBL; AF313390; AAG32063.1; -; mRNA.
DR EMBL; AE014296; AAN12114.1; -; Genomic_DNA.
DR EMBL; AE014296; AAN12115.1; -; Genomic_DNA.
DR EMBL; AE014296; AAO41277.1; -; Genomic_DNA.
DR EMBL; AY075339; AAL68204.1; -; mRNA.
DR RefSeq; NP_001027111.1; NM_001031940.2.
DR RefSeq; NP_001027112.1; NM_001031941.2.
DR RefSeq; NP_001027113.1; NM_001031942.2.
DR AlphaFoldDB; Q9GN97; -.
DR SMR; Q9GN97; -.
DR STRING; 7227.FBpp0113069; -.
DR GlyGen; Q9GN97; 1 site.
DR PaxDb; Q9GN97; -.
DR EnsemblMetazoa; FBtr0091709; FBpp0099635; FBgn0260458.
DR EnsemblMetazoa; FBtr0091710; FBpp0099636; FBgn0260458.
DR EnsemblMetazoa; FBtr0091711; FBpp0099637; FBgn0260458.
DR GeneID; 3771920; -.
DR KEGG; dme:Dmel_CG33717; -.
DR CTD; 3771920; -.
DR FlyBase; FBgn0260458; PGRP-LD.
DR VEuPathDB; VectorBase:FBgn0260458; -.
DR eggNOG; ENOG502TBSX; Eukaryota.
DR GeneTree; ENSGT00940000166535; -.
DR HOGENOM; CLU_075160_0_0_1; -.
DR InParanoid; Q9GN97; -.
DR OMA; CETYEAR; -.
DR PhylomeDB; Q9GN97; -.
DR Reactome; R-DME-6803157; Antimicrobial peptides.
DR BioGRID-ORCS; 3771920; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 3771920; -.
DR PRO; PR:Q9GN97; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0260458; Expressed in brain and 9 other tissues.
DR ExpressionAtlas; Q9GN97; baseline.
DR Genevisible; Q9GN97; DM.
DR GO; GO:0005887; C:integral component of plasma membrane; NAS:UniProtKB.
DR GO; GO:0042834; F:peptidoglycan binding; NAS:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0045087; P:innate immune response; NAS:UniProtKB.
DR CDD; cd06583; PGRP; 1.
DR Gene3D; 3.40.80.10; -; 1.
DR InterPro; IPR036505; Amidase/PGRP_sf.
DR InterPro; IPR002502; Amidase_domain.
DR InterPro; IPR015510; PGRP.
DR InterPro; IPR006619; PGRP_domain_met/bac.
DR PANTHER; PTHR11022; PTHR11022; 2.
DR SMART; SM00701; PGRP; 1.
DR SUPFAM; SSF55846; SSF55846; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; Glycoprotein; Immunity; Innate immunity;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..282
FT /note="Peptidoglycan-recognition protein LD"
FT /id="PRO_0000220625"
FT TOPO_DOM 1..88
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..282
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 222
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 162..166
FT /evidence="ECO:0000250"
SQ SEQUENCE 282 AA; 31104 MW; 9B1783995E006751 CRC64;
MDSSHIAVRV ARRSPSPAAV SQSSYGSLGS SQDIHIRVDK EGVASESTPL LAAAQRSIKT
SSSLTASVSA SSTTPSNCRR NPTLHEDCFN WRSVGLLVMC ASALALAAYL LWRQTQTPDF
GYRLSIVGHG IWSDMELQGR GTLFDPIGVG TVIFTHTGSN ECHDDCPDVL HKLERSHVGE
LPYNFLVAGD CQVFEAQGWH YRSQYPRDLN GIDSLVMAFV GNFSGRPPID CQLMAAQALI
LESLKRRILQ PIYQLFVLGS YTDALQRELR HWPHYASHQT SK
