PGR5_ARATH
ID PGR5_ARATH Reviewed; 133 AA.
AC Q9SL05; Q8LE53;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Protein PROTON GRADIENT REGULATION 5, chloroplastic;
DE Flags: Precursor;
GN Name=PGR5; OrderedLocusNames=At2g05620; ORFNames=T20G20.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, MUTAGENESIS OF GLY-130, AND SUBCELLULAR LOCATION.
RX PubMed=12176323; DOI=10.1016/s0092-8674(02)00867-x;
RA Munekage Y., Hojo M., Meurer J., Endo T., Tasaka M., Shikanai T.;
RT "PGR5 is involved in cyclic electron flow around photosystem I and is
RT essential for photoprotection in Arabidopsis.";
RL Cell 110:361-371(2002).
RN [6]
RP FUNCTION.
RX PubMed=17803955; DOI=10.1016/j.bbabio.2007.07.007;
RA Nandha B., Finazzi G., Joliot P., Hald S., Johnson G.N.;
RT "The role of PGR5 in the redox poising of photosynthetic electron
RT transport.";
RL Biochim. Biophys. Acta 1767:1252-1259(2007).
RN [7]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17913767; DOI=10.1093/pcp/pcm116;
RA Okegawa Y., Long T.A., Iwano M., Takayama S., Kobayashi Y., Covert S.F.,
RA Shikanai T.;
RT "A balanced PGR5 level is required for chloroplast development and optimum
RT operation of cyclic electron transport around photosystem I.";
RL Plant Cell Physiol. 48:1462-1471(2007).
RN [8]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH PGRL1A AND PGRL1B.
RC STRAIN=cv. Columbia;
RX PubMed=18243102; DOI=10.1016/j.cell.2007.12.028;
RA DalCorso G., Pesaresi P., Masiero S., Aseeva E., Schuenemann D.,
RA Finazzi G., Joliot P., Barbato R., Leister D.;
RT "A complex containing PGRL1 and PGR5 is involved in the switch between
RT linear and cyclic electron flow in Arabidopsis.";
RL Cell 132:273-285(2008).
RN [9]
RP INDUCTION BY DROUGHT.
RX PubMed=20392519; DOI=10.1016/j.jplph.2010.02.006;
RA Lehtimaeki N., Lintala M., Allahverdiyeva Y., Aro E.M., Mulo P.;
RT "Drought stress-induced upregulation of components involved in ferredoxin-
RT dependent cyclic electron transfer.";
RL J. Plant Physiol. 167:1018-1022(2010).
RN [10]
RP FUNCTION, INTERACTION WITH PGRL1A, AND DISULFIDE BOND.
RX PubMed=23290914; DOI=10.1016/j.molcel.2012.11.030;
RA Hertle A.P., Blunder T., Wunder T., Pesaresi P., Pribil M., Armbruster U.,
RA Leister D.;
RT "PGRL1 is the elusive ferredoxin-plastoquinone reductase in photosynthetic
RT cyclic electron flow.";
RL Mol. Cell 49:511-523(2013).
CC -!- FUNCTION: Involved in the regulation of the cyclic electron flow (CEF)
CC around Photosystem I. Essential for the reduction of PGRL1A by
CC ferredoxin and for photoprotection. {ECO:0000269|PubMed:12176323,
CC ECO:0000269|PubMed:17803955, ECO:0000269|PubMed:17913767,
CC ECO:0000269|PubMed:23290914}.
CC -!- SUBUNIT: Interacts with PGRL1A and PGRL1B.
CC {ECO:0000269|PubMed:18243102, ECO:0000269|PubMed:23290914}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:12176323, ECO:0000269|PubMed:17913767,
CC ECO:0000269|PubMed:18243102}; Peripheral membrane protein
CC {ECO:0000269|PubMed:12176323, ECO:0000269|PubMed:17913767,
CC ECO:0000269|PubMed:18243102}; Stromal side
CC {ECO:0000269|PubMed:12176323, ECO:0000269|PubMed:17913767,
CC ECO:0000269|PubMed:18243102}.
CC -!- INDUCTION: Up-regulated by drought stress.
CC {ECO:0000269|PubMed:20392519}.
CC -!- PTM: Disulfide bonds; Cys-11 and Cys-105 are probably involved in the
CC formation of disulfide bridges with 'Cys-300' and 'Cys-303' of PGRL1A.
CC 'Cys-272' and 'Cys-275' of PGRL1A may also be used to form the
CC disulfide bridges, but in this case the cyclic electron flow is lost.
CC -!- SIMILARITY: Belongs to the PGR5 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC006220; AAD24646.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05957.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61532.1; -; Genomic_DNA.
DR EMBL; AF375454; AAK53038.1; -; mRNA.
DR EMBL; AY060546; AAL31177.1; -; mRNA.
DR EMBL; AY085613; AAM62834.1; -; mRNA.
DR PIR; G84470; G84470.
DR RefSeq; NP_001318202.1; NM_001335284.1.
DR RefSeq; NP_565327.1; NM_126585.6.
DR AlphaFoldDB; Q9SL05; -.
DR SMR; Q9SL05; -.
DR BioGRID; 511; 6.
DR IntAct; Q9SL05; 3.
DR STRING; 3702.AT2G05620.1; -.
DR PaxDb; Q9SL05; -.
DR PRIDE; Q9SL05; -.
DR ProteomicsDB; 235049; -.
DR EnsemblPlants; AT2G05620.1; AT2G05620.1; AT2G05620.
DR EnsemblPlants; AT2G05620.2; AT2G05620.2; AT2G05620.
DR GeneID; 815111; -.
DR Gramene; AT2G05620.1; AT2G05620.1; AT2G05620.
DR Gramene; AT2G05620.2; AT2G05620.2; AT2G05620.
DR KEGG; ath:AT2G05620; -.
DR Araport; AT2G05620; -.
DR TAIR; locus:2058974; AT2G05620.
DR eggNOG; ENOG502S7VD; Eukaryota.
DR HOGENOM; CLU_124691_0_0_1; -.
DR InParanoid; Q9SL05; -.
DR OMA; IRAQPMM; -.
DR OrthoDB; 1562064at2759; -.
DR PhylomeDB; Q9SL05; -.
DR PRO; PR:Q9SL05; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SL05; baseline and differential.
DR Genevisible; Q9SL05; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; IDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0009055; F:electron transfer activity; IMP:TAIR.
DR GO; GO:0071484; P:cellular response to light intensity; IMP:TAIR.
DR GO; GO:0010117; P:photoprotection; IMP:TAIR.
DR GO; GO:0009773; P:photosynthetic electron transport in photosystem I; IMP:TAIR.
DR GO; GO:0009644; P:response to high light intensity; IMP:TAIR.
DR GO; GO:0009414; P:response to water deprivation; IMP:TAIR.
DR InterPro; IPR037497; PGR5.
DR PANTHER; PTHR35709; PTHR35709; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Disulfide bond; Electron transport; Membrane; Plastid;
KW Reference proteome; Thylakoid; Transit peptide; Transport.
FT TRANSIT 1..60
FT /note="Chloroplast"
FT /evidence="ECO:0000305"
FT CHAIN 61..133
FT /note="Protein PROTON GRADIENT REGULATION 5, chloroplastic"
FT /id="PRO_0000322591"
FT MUTAGEN 130
FT /note="G->S: In pgr5; increased instability of the
FT protein."
FT /evidence="ECO:0000269|PubMed:12176323"
FT CONFLICT 16
FT /note="I -> L (in Ref. 4; AAM62834)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 133 AA; 14293 MW; 068C64C417C50A54 CRC64;
MAAASISAIG CNQTLIGTSF YGGWGSSISG EDYQTMLSKT VAPPQQARVS RKAIRAVPMM
KNVNEGKGLF APLVVVTRNL VGKKRFNQLR GKAIALHSQV ITEFCKSIGA DAKQRQGLIR
LAKKNGERLG FLA
