PGRC2_RAT
ID PGRC2_RAT Reviewed; 217 AA.
AC Q5XIU9;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Membrane-associated progesterone receptor component 2 {ECO:0000305};
GN Name=Pgrmc2 {ECO:0000312|RGD:1308804};
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-98; SER-202 AND THR-205, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Required for the maintenance of uterine histoarchitecture and
CC normal female reproductive lifespan. May serve as a universal non-
CC classical progesterone receptor in the uterus. Intracellular heme
CC chaperone required for delivery of labile, or signaling heme, to the
CC nucleus. Plays a role in adipocyte function and systemic glucose
CC homeostasis. In brown fat, which has a high demand for heme, delivery
CC of labile heme in the nucleus regulates the activity of heme-responsive
CC transcriptional repressors such as NR1D1 and BACH1.
CC {ECO:0000250|UniProtKB:Q80UU9}.
CC -!- SUBUNIT: Interacts with PGRMC1 (By similarity). Interacts with AAAS (By
CC similarity). {ECO:0000250|UniProtKB:O15173,
CC ECO:0000250|UniProtKB:Q80UU9}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q80UU9}; Single-
CC pass membrane protein {ECO:0000250|UniProtKB:Q80UU9}. Nucleus envelope
CC {ECO:0000250|UniProtKB:O15173}. Endoplasmic reticulum
CC {ECO:0000250|UniProtKB:O15173}.
CC -!- DOMAIN: The cytochrome b5 heme-binding domain lacks the conserved iron-
CC binding His residues at positions 131 and 155. {ECO:0000250}.
CC -!- MISCELLANEOUS: Non-classical progesterone receptors involved in
CC extranuclear signaling are classified in 2 groups: the class II
CC progestin and adipoQ receptor (PAQR) family (also called mPRs) (PAQR5,
CC PAQR6, PAQR7, PAQR8 and PAQR9) and the b5-like heme/steroid-binding
CC protein family (also called MAPRs) (PGRMC1, PGRMC2, NENF and CYB5D2).
CC {ECO:0000250|UniProtKB:O15173}.
CC -!- SIMILARITY: Belongs to the cytochrome b5 family. MAPR subfamily.
CC {ECO:0000305}.
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DR EMBL; BC083571; AAH83571.1; -; mRNA.
DR RefSeq; NP_001008375.1; NM_001008374.1.
DR AlphaFoldDB; Q5XIU9; -.
DR SMR; Q5XIU9; -.
DR IntAct; Q5XIU9; 1.
DR STRING; 10116.ENSRNOP00000018796; -.
DR iPTMnet; Q5XIU9; -.
DR PhosphoSitePlus; Q5XIU9; -.
DR jPOST; Q5XIU9; -.
DR PaxDb; Q5XIU9; -.
DR PRIDE; Q5XIU9; -.
DR Ensembl; ENSRNOT00000018796; ENSRNOP00000018796; ENSRNOG00000014051.
DR GeneID; 361940; -.
DR KEGG; rno:361940; -.
DR UCSC; RGD:1308804; rat.
DR CTD; 10424; -.
DR RGD; 1308804; Pgrmc2.
DR eggNOG; KOG1110; Eukaryota.
DR GeneTree; ENSGT00940000159744; -.
DR HOGENOM; CLU_042860_1_0_1; -.
DR InParanoid; Q5XIU9; -.
DR OMA; MAVNMKI; -.
DR OrthoDB; 1331617at2759; -.
DR PhylomeDB; Q5XIU9; -.
DR TreeFam; TF314562; -.
DR Reactome; R-RNO-8980692; RHOA GTPase cycle.
DR Reactome; R-RNO-9013404; RAC2 GTPase cycle.
DR Reactome; R-RNO-9013405; RHOD GTPase cycle.
DR Reactome; R-RNO-9013408; RHOG GTPase cycle.
DR Reactome; R-RNO-9013423; RAC3 GTPase cycle.
DR PRO; PR:Q5XIU9; -.
DR Proteomes; UP000002494; Chromosome 2.
DR Bgee; ENSRNOG00000014051; Expressed in liver and 20 other tissues.
DR Genevisible; Q5XIU9; RN.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005635; C:nuclear envelope; ISS:UniProtKB.
DR GO; GO:0020037; F:heme binding; ISS:UniProtKB.
DR GO; GO:0015232; F:heme transmembrane transporter activity; ISO:RGD.
DR GO; GO:0005496; F:steroid binding; IEA:UniProtKB-KW.
DR GO; GO:0060612; P:adipose tissue development; ISS:UniProtKB.
DR GO; GO:0015886; P:heme transport; ISO:RGD.
DR Gene3D; 3.10.120.10; -; 1.
DR InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR Pfam; PF00173; Cyt-b5; 1.
DR SMART; SM01117; Cyt-b5; 1.
DR SUPFAM; SSF55856; SSF55856; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Lipid-binding; Membrane; Nucleus; Phosphoprotein;
KW Receptor; Reference proteome; Steroid-binding; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..217
FT /note="Membrane-associated progesterone receptor component
FT 2"
FT /id="PRO_0000121745"
FT TRANSMEM 40..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 96..195
FT /note="Cytochrome b5 heme-binding"
FT REGION 196..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 84
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O15173"
FT MOD_RES 98
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 202
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 204
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:O15173"
FT MOD_RES 205
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 217 AA; 23403 MW; F70E1B00DDAC71F7 CRC64;
MAAGDGDVKL STLGSGGERG GDGSPGGAGA TAARSSWVAA LLATGGEMLL NVALVALVLL
GAYRLWVRWG RRGLCSGPGA GEESPAATLP RMKKRDFSLE QLRQYDGART PRILLAVNGK
VFDVTKGSKF YGPAGPYGIF AGRDASRGLA TFCLDKDALR DEYDDLSDLN AVQMESVREW
EMQFKEKYDY VGRLLKPGEE PSEYTDEEDT KDHSKQD
