PGTA_SALTY
ID PGTA_SALTY Reviewed; 415 AA.
AC P06184;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 3.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Phosphoglycerate transport system transcriptional regulatory protein PgtA;
GN Name=pgtA; OrderedLocusNames=STM2396;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3023201; DOI=10.1016/0378-1119(86)90131-9;
RA Yu G.-Q., Hong J.-S.;
RT "Identification and nucleotide sequence of the activator gene of the
RT externally induced phosphoglycerate transport system of Salmonella
RT typhimurium.";
RL Gene 45:51-57(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
RX PubMed=2842311; DOI=10.1128/jb.170.9.4299-4303.1988;
RA Yang Y.L., Goldrick D., Hong J.-S.;
RT "Identification of the products and nucleotide sequences of two regulatory
RT genes involved in the exogenous induction of phosphoglycerate transport in
RT Salmonella typhimurium.";
RL J. Bacteriol. 170:4299-4303(1988).
CC -!- FUNCTION: Member of the two-component regulatory system PgtB/PgtA that
CC regulates the inducible phosphoglycerate transport system. When
CC activated by PgtB it acts in conjunction with sigma-54 as a
CC transcriptional activator.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- PTM: Phosphorylated by PgtB. {ECO:0000305}.
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DR EMBL; M13923; AAA27184.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL21296.1; -; Genomic_DNA.
DR EMBL; M21279; AAA68216.1; -; Genomic_DNA.
DR PIR; A28255; A28255.
DR RefSeq; NP_461337.1; NC_003197.2.
DR RefSeq; WP_000930843.1; NC_003197.2.
DR AlphaFoldDB; P06184; -.
DR SMR; P06184; -.
DR STRING; 99287.STM2396; -.
DR PaxDb; P06184; -.
DR EnsemblBacteria; AAL21296; AAL21296; STM2396.
DR GeneID; 1253918; -.
DR KEGG; stm:STM2396; -.
DR PATRIC; fig|99287.12.peg.2534; -.
DR HOGENOM; CLU_000445_0_5_6; -.
DR OMA; DIYMPAM; -.
DR PhylomeDB; P06184; -.
DR BioCyc; SENT99287:STM2396-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR002197; HTH_Fis.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR002078; Sigma_54_int.
DR InterPro; IPR025944; Sigma_54_int_dom_CS.
DR Pfam; PF02954; HTH_8; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF14532; Sigma54_activ_2; 1.
DR SMART; SM00448; REC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
DR PROSITE; PS00688; SIGMA54_INTERACT_3; 1.
DR PROSITE; PS50045; SIGMA54_INTERACT_4; 1.
PE 3: Inferred from homology;
KW Activator; ATP-binding; Cytoplasm; DNA-binding; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation; Two-component regulatory system.
FT CHAIN 1..415
FT /note="Phosphoglycerate transport system transcriptional
FT regulatory protein PgtA"
FT /id="PRO_0000081185"
FT DOMAIN 7..121
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DOMAIN 142..341
FT /note="Sigma-54 factor interaction"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT DNA_BIND 385..404
FT /note="H-T-H motif"
FT /evidence="ECO:0000255"
FT BINDING 170..177
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00193"
FT MOD_RES 56
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT CONFLICT 108..111
FT /note="VDPG -> SIRA (in Ref. 1; AAA27184)"
FT /evidence="ECO:0000305"
FT CONFLICT 406
FT /note="Y -> D (in Ref. 1; AAA27184)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 415 AA; 47526 MW; 00A540568884515B CRC64;
MLNDECSILL IDDDVDVLDA YTQMLEQAGY RVRGFTHPFE AKEWVKADWE GIVLSDVCMP
GCSGIDLMTL FHQDDDQLPI LLITGHGDVP MAVDAVKKGA WDFLQKPVDP GKLLILIEDA
LRQRRSVIAR RQYCQQTLQV ELIGRSEWMN QFRQRLQQLA ETDIAVWFYG EHGTGRMTGA
RYLHQLGRNA KGPFVRYELT PENAGQLETF IDQAQGGTLV LSHPEYLTRE QQHHLARLQS
LEHRPFRLVG VGSASLVEQA AANQIAAELY YCFAMTQIAC QSLSQRPDDI EPLFRHYLRK
ACLRLNHPVP EIAGELLKGI MRRAWPSNVR ELANAAELFA VGVLPLAETV NPQLLLQEPT
PLDRRVEEYE RQIITEALNI HQGRINEVAE YLQIPRKKLY LRMKKYGLSK EHYKF
