PGTA_YEAST
ID PGTA_YEAST Reviewed; 327 AA.
AC Q00618; D6VWF1;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 30-AUG-2005, sequence version 3.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Geranylgeranyl transferase type-2 subunit alpha;
DE EC=2.5.1.60;
DE AltName: Full=GGTase-II-alpha;
DE AltName: Full=Geranylgeranyl transferase type II subunit alpha;
DE AltName: Full=PGGT;
DE AltName: Full=Type II protein geranyl-geranyltransferase subunit alpha;
DE AltName: Full=YPT1/SEC4 proteins geranylgeranyltransferase subunit alpha;
GN Name=BET4; OrderedLocusNames=YJL031C; ORFNames=J1254;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1651172; DOI=10.1016/0092-8674(81)90015-5;
RA Li R., Murray A.W.;
RT "Feedback control of mitosis in budding yeast.";
RL Cell 66:519-531(1991).
RN [2]
RP SEQUENCE REVISION TO 171.
RA Li R., Chen R.-H., Murray A.W.;
RL Submitted (AUG-1994) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP REVISION OF GENE MODEL.
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=12844361; DOI=10.1186/gb-2003-4-7-r45;
RA Brachat S., Dietrich F.S., Voegeli S., Zhang Z., Stuart L., Lerch A.,
RA Gates K., Gaffney T.D., Philippsen P.;
RT "Reinvestigation of the Saccharomyces cerevisiae genome annotation by
RT comparison to the genome of a related fungus: Ashbya gossypii.";
RL Genome Biol. 4:R45.1-R45.13(2003).
RN [6]
RP CHARACTERIZATION.
RX PubMed=8232541; DOI=10.1038/366082a0;
RA Li R., Havel C., Watson J.A., Murray A.W.;
RT "The mitotic feedback control gene MAD2 encodes the alpha-subunit of a
RT prenyltransferase.";
RL Nature 366:82-84(1993).
RN [7]
RP ERRATUM OF PUBMED:8232541.
RA Li R., Havel C., Watson J.A., Murray A.W.;
RL Nature 371:438-438(1994).
RN [8]
RP CHARACTERIZATION.
RX PubMed=8232542; DOI=10.1038/366084a0;
RA Jiang Y., Rossi G., Ferro-Novick S.;
RT "Bet2p and Mad2p are components of a prenyltransferase that adds
RT geranylgeranyl onto Ypt1p and Sec4p.";
RL Nature 366:84-86(1993).
CC -!- FUNCTION: Catalyzes the transfer of a geranyl-geranyl moiety from
CC geranyl-geranyl pyrophosphate to proteins having the C-terminal -XCC or
CC -XCXC, where both cysteines may become modified. Acts on YPT1 and SEC4.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=geranylgeranyl diphosphate + L-cysteinyl-[protein] =
CC diphosphate + S-geranylgeranyl-L-cysteinyl-[protein];
CC Xref=Rhea:RHEA:21240, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:11537,
CC ChEBI:CHEBI:29950, ChEBI:CHEBI:33019, ChEBI:CHEBI:57533,
CC ChEBI:CHEBI:86021; EC=2.5.1.60;
CC -!- SUBUNIT: Heterodimer of an alpha and a beta subunit.
CC -!- INTERACTION:
CC Q00618; P20133: BET2; NbExp=3; IntAct=EBI-3573, EBI-3559;
CC -!- SIMILARITY: Belongs to the protein prenyltransferase subunit alpha
CC family. {ECO:0000305}.
CC -!- CAUTION: Was originally thought to be MAD2.
CC {ECO:0000305|PubMed:1651172}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA21386.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAA89323.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U14132; AAA21386.1; ALT_SEQ; Genomic_DNA.
DR EMBL; Z49307; CAA89323.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BK006943; DAA08767.1; -; Genomic_DNA.
DR PIR; S48301; S48301.
DR RefSeq; NP_012503.2; NM_001181465.1.
DR AlphaFoldDB; Q00618; -.
DR SMR; Q00618; -.
DR BioGRID; 33728; 726.
DR ComplexPortal; CPX-1636; Protein geranylgeranyltransferase type II complex.
DR DIP; DIP-4994N; -.
DR IntAct; Q00618; 2.
DR STRING; 4932.YJL031C; -.
DR MaxQB; Q00618; -.
DR PaxDb; Q00618; -.
DR PRIDE; Q00618; -.
DR EnsemblFungi; YJL031C_mRNA; YJL031C; YJL031C.
DR GeneID; 853421; -.
DR KEGG; sce:YJL031C; -.
DR SGD; S000003568; BET4.
DR VEuPathDB; FungiDB:YJL031C; -.
DR eggNOG; KOG0529; Eukaryota.
DR GeneTree; ENSGT00550000075121; -.
DR HOGENOM; CLU_031996_0_0_1; -.
DR InParanoid; Q00618; -.
DR OMA; EYYTVWN; -.
DR BioCyc; YEAST:MON3O-3; -.
DR Reactome; R-SCE-6803205; TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain.
DR Reactome; R-SCE-8873719; RAB geranylgeranylation.
DR PRO; PR:Q00618; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; Q00618; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0005777; C:peroxisome; IDA:SGD.
DR GO; GO:0005968; C:Rab-protein geranylgeranyltransferase complex; IDA:SGD.
DR GO; GO:0004663; F:Rab geranylgeranyltransferase activity; IDA:SGD.
DR GO; GO:0031267; F:small GTPase binding; ISS:UniProtKB.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IMP:SGD.
DR GO; GO:0018344; P:protein geranylgeranylation; IDA:SGD.
DR GO; GO:0006612; P:protein targeting to membrane; IMP:SGD.
DR InterPro; IPR002088; Prenyl_trans_a.
DR InterPro; IPR032955; RabGGTase_alpha.
DR PANTHER; PTHR11129:SF2; PTHR11129:SF2; 1.
DR Pfam; PF01239; PPTA; 4.
DR PROSITE; PS51147; PFTA; 5.
PE 1: Evidence at protein level;
KW Prenyltransferase; Reference proteome; Repeat; Transferase.
FT CHAIN 1..327
FT /note="Geranylgeranyl transferase type-2 subunit alpha"
FT /id="PRO_0000119756"
FT REPEAT 44..78
FT /note="PFTA 1"
FT REPEAT 84..118
FT /note="PFTA 2"
FT REPEAT 123..157
FT /note="PFTA 3"
FT REPEAT 163..197
FT /note="PFTA 4"
FT REPEAT 207..241
FT /note="PFTA 5"
SQ SEQUENCE 327 AA; 39675 MW; A7C3E16C14DDD09C CRC64;
MHGIKRKQWT KELLRQKRVQ DEKKIYDYRS LTENVLNMRD EKIYSIEALK KTSELLEKNP
EFNAIWNYRR DIIASLASEL EIPFWDKELV FVMMLLKDYP KVYWIWNHRL WVLKHYPTSS
PKVWQTELAV VNKLLEQDAR NYHGWHYRRI VVGNIESITN KSLDKEEFEY TTIKINNNIS
NYSAWHQRVQ IISRMFQKGE VGNQKEYIRT EISYIINAMF TDAEDQSVWF YIKWFIKNDI
VCKTLDEQEY LKMLKDLREN ILLINNDEIE FSGKQNIWCL KILLVLEDIL EEKEALTERS
SEQYLVQLID ADPLRKNRYL HLLEQHK
