位置:首页 > 蛋白库 > PGXB_ASPFN
PGXB_ASPFN
ID   PGXB_ASPFN              Reviewed;         435 AA.
AC   B8NX60;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Probable exopolygalacturonase B;
DE            EC=3.2.1.67;
DE   AltName: Full=Galacturan 1,4-alpha-galacturonidase B;
DE   AltName: Full=Poly(1,4-alpha-D-galacturonide)galacturonohydrolase B;
DE   Flags: Precursor;
GN   Name=pgxB; ORFNames=AFLA_122480;
OS   Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS   / JCM 12722 / SRRC 167).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=332952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC   167;
RX   PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA   Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA   Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT   "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT   aflatoxin contamination of food and feed.";
RL   Genome Announc. 3:E0016815-E0016815(2015).
CC   -!- FUNCTION: Specific in hydrolyzing the terminal glycosidic bond of
CC       polygalacturonic acid and oligogalacturonates. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[(1->4)-alpha-D-galacturonosyl](n) + H2O = [(1->4)-alpha-D-
CC         galacturonosyl](n-1) + alpha-D-galacturonate; Xref=Rhea:RHEA:14117,
CC         Rhea:RHEA-COMP:14570, Rhea:RHEA-COMP:14572, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:58658, ChEBI:CHEBI:140523; EC=3.2.1.67;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 28 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EQ963485; EED46018.1; -; Genomic_DNA.
DR   RefSeq; XP_002384954.1; XM_002384913.1.
DR   AlphaFoldDB; B8NX60; -.
DR   SMR; B8NX60; -.
DR   STRING; 5059.CADAFLAP00012819; -.
DR   EnsemblFungi; EED46018; EED46018; AFLA_122480.
DR   VEuPathDB; FungiDB:AFLA_122480; -.
DR   eggNOG; ENOG502QPPR; Eukaryota.
DR   HOGENOM; CLU_016031_1_0_1; -.
DR   OMA; VIENLWM; -.
DR   Proteomes; UP000001875; Unassembled WGS sequence.
DR   GO; GO:0005576; C:extracellular region; ISS:UniProtKB.
DR   GO; GO:0047911; F:galacturan 1,4-alpha-galacturonidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004650; F:polygalacturonase activity; ISS:UniProtKB.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0045490; P:pectin catabolic process; ISS:UniProtKB.
DR   Gene3D; 2.160.20.10; -; 1.
DR   InterPro; IPR000743; Glyco_hydro_28.
DR   InterPro; IPR012334; Pectin_lyas_fold.
DR   InterPro; IPR011050; Pectin_lyase_fold/virulence.
DR   Pfam; PF00295; Glyco_hydro_28; 1.
DR   SUPFAM; SSF51126; SSF51126; 1.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Disulfide bond; Glycoprotein;
KW   Glycosidase; Hydrolase; Repeat; Secreted; Signal.
FT   SIGNAL          1..15
FT                   /evidence="ECO:0000255"
FT   CHAIN           16..435
FT                   /note="Probable exopolygalacturonase B"
FT                   /id="PRO_0000393676"
FT   REPEAT          208..239
FT                   /note="PbH1 1"
FT   REPEAT          240..261
FT                   /note="PbH1 2"
FT   REPEAT          262..283
FT                   /note="PbH1 3"
FT   REPEAT          294..315
FT                   /note="PbH1 4"
FT   REPEAT          326..347
FT                   /note="PbH1 5"
FT   REPEAT          366..388
FT                   /note="PbH1 6"
FT   ACT_SITE        254
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        277
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        59
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        184
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        224
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        262
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        274
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        301
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        328
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        365
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        373
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        406
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        256..273
FT                   /evidence="ECO:0000250"
FT   DISULFID        391..397
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   435 AA;  48372 MW;  C4C232FD9050B524 CRC64;
     MKFFLATLFA SAVSSIAVDR LIPGAQIIPE SDTRALLRVG GHHDKYHDRR TITIRPSRND
     TDDVSRDFLW GIKHANHGGR LLLKKGEKYV IGRKLDLTFL DDIEVQLDGE LKFTDDVSYW
     QENNFYYDFQ KSITFWRWGG KDIKIFGTGL LNGNGQRWYN EFAGQEILDP DNEYYRPILF
     LTENATRISV EGITQLNSPC WTNFFIQSKD VSFDDVYIHA FSTNKSALPK NSDGFDSLNV
     DGLTVTNTRV DVGDDCFSPK PNTTNIFVQN LLCNNTHGVS MGSIGQYPGV MDIIEHAYIE
     NVTLLNGQNG ARLKAWAGQD VGYGRINNIT YKNIRIENTD APVVLDQCYF DIEAAECAQY
     PSQVNVTNIL FENISGTSSG KNGKVVADLV CSPNAVCSDI QLKNINLTSP AGSPPEIVCE
     GVQGDIGVEC QASAD
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024