PH1_ARATH
ID PH1_ARATH Reviewed; 145 AA.
AC Q9ST43; O82386;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 2.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Pleckstrin homology domain-containing protein 1;
DE Short=AtPH1;
GN Name=PH1; OrderedLocusNames=At2g29700; ORFNames=T27A16.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RA Mikami K., Takahashi S., Katagiri T., Yamaguchi-Shinozaki K., Shinozaki K.;
RT "Isolation of an Arabidopsis thaliana cDNA encoding a pleckstrin homology
RT domain protein, a putative homologue of human pleckstrin.";
RL J. Exp. Bot. 50:729-730(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=11001876; DOI=10.1042/0264-6021:3510019;
RA Dowler S.J., Currie R.A., Campbell D.G., Deak M., Kular G., Downes C.P.,
RA Alessi D.R.;
RT "Identification of pleckstrin-homology-domain-containing proteins with
RT novel phosphoinositide-binding specificities.";
RL Biochem. J. 351:19-31(2000).
CC -!- FUNCTION: Binds specifically to phosphatidylinositol 3-phosphate
CC (PtdIns3P), but not to other phosphoinositides.
CC {ECO:0000269|PubMed:11001876}.
CC -!- SUBUNIT: Binds PtdIns3P.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed. {ECO:0000269|Ref.1}.
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DR EMBL; AB005903; BAA84651.1; -; mRNA.
DR EMBL; AC005496; AAC35236.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08295.1; -; Genomic_DNA.
DR EMBL; AY050344; AAK91361.1; -; mRNA.
DR EMBL; AY094043; AAM16199.1; -; mRNA.
DR EMBL; AY084905; AAM61468.1; -; mRNA.
DR PIR; E84699; E84699.
DR PIR; T52471; T52471.
DR RefSeq; NP_565687.1; NM_128524.4.
DR AlphaFoldDB; Q9ST43; -.
DR SMR; Q9ST43; -.
DR STRING; 3702.AT2G29700.1; -.
DR iPTMnet; Q9ST43; -.
DR PaxDb; Q9ST43; -.
DR PRIDE; Q9ST43; -.
DR ProteomicsDB; 236433; -.
DR EnsemblPlants; AT2G29700.1; AT2G29700.1; AT2G29700.
DR GeneID; 817520; -.
DR Gramene; AT2G29700.1; AT2G29700.1; AT2G29700.
DR KEGG; ath:AT2G29700; -.
DR Araport; AT2G29700; -.
DR TAIR; locus:2060625; AT2G29700.
DR eggNOG; ENOG502RXGP; Eukaryota.
DR HOGENOM; CLU_111315_0_0_1; -.
DR InParanoid; Q9ST43; -.
DR OMA; MESIWRI; -.
DR OrthoDB; 1459722at2759; -.
DR PhylomeDB; Q9ST43; -.
DR PRO; PR:Q9ST43; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ST43; baseline and differential.
DR Genevisible; Q9ST43; AT.
DR GO; GO:0005777; C:peroxisome; HDA:TAIR.
DR GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:GO_Central.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR045188; Boi1/Boi2-like.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR PANTHER; PTHR22902; PTHR22902; 1.
DR Pfam; PF00169; PH; 1.
DR SMART; SM00233; PH; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Lipid-binding; Reference proteome.
FT CHAIN 1..145
FT /note="Pleckstrin homology domain-containing protein 1"
FT /id="PRO_0000053905"
FT DOMAIN 26..127
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 29..53
FT /note="Binds specifically PtdIns3P"
FT CONFLICT 4
FT /note="I -> M (in Ref. 1; BAA84651)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 145 AA; 16811 MW; D9A5B7E724C8087A CRC64;
MESIWRIATG QDPSREDYEG IEFWSNPERS GWLTKQGDYI KTWRRRWFVL KRGKLLWFKD
QAAAGIRGST PRGVISVGDC LTVKGAEDVV NKPFAFELSS GSYTMFFIAD NEKEKEEWIN
SIGRSIVQHS RSVTDSEVLD YDHRR
