PHAAB_RHIME
ID PHAAB_RHIME Reviewed; 999 AA.
AC Q52978; Q52979; Q8RTE9; Q92LV7;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Probable K(+)/H(+) antiporter subunit A/B;
DE AltName: Full=pH adaptation potassium efflux system protein A/B;
DE Short=Pha system subunit A/B;
GN Name=phaAB; Synonyms=phaA, phaA1, phaB; OrderedLocusNames=R02910;
GN ORFNames=SMc03179;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=41;
RX PubMed=9680201; DOI=10.1046/j.1365-2958.1998.00868.x;
RA Putnoky P., Kereszt A., Nakamura T., Endre G., Grosskopf E., Kiss P.,
RA Kondorosi A.;
RT "The pha gene cluster of Rhizobium meliloti involved in pH adaptation and
RT symbiosis encodes a novel type of K+ efflux system.";
RL Mol. Microbiol. 28:1091-1101(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-926.
RC STRAIN=JJ1c10;
RA Watson R.J., Heys R.;
RT "Sinorhizobium meliloti phaA gene segment.";
RL Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Part of a K(+) efflux system which is required for the
CC adaptation of R.meliloti to alkaline pH as well as for the infection
CC process during symbiotic nodule development.
CC -!- SUBUNIT: May form a heterooligomeric complex that consists of six
CC subunits: PhaAB, PhaC, PhaD, PhaE, PhaF and PhaG.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the CPA3 antiporters
CC (TC 2.A.63) subunit A family. {ECO:0000305}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the CPA3 antiporters
CC (TC 2.A.63) subunit B family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA63735.1; Type=Frameshift; Note=Produces two separate ORFs named phaA and phaB.; Evidence={ECO:0000305};
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DR EMBL; X93358; CAA63734.1; ALT_FRAME; Genomic_DNA.
DR EMBL; X93358; CAA63735.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AL591688; CAC47489.1; -; Genomic_DNA.
DR EMBL; AF465791; AAL76258.1; -; Genomic_DNA.
DR RefSeq; NP_387016.1; NC_003047.1.
DR RefSeq; WP_010970284.1; NC_003047.1.
DR AlphaFoldDB; Q52978; -.
DR SMR; Q52978; -.
DR STRING; 266834.SMc03179; -.
DR TCDB; 2.A.63.1.1; the monovalent cation (k(+) or na(+)):proton antiporter-3 (cpa3) family.
DR PRIDE; Q52978; -.
DR EnsemblBacteria; CAC47489; CAC47489; SMc03179.
DR GeneID; 61604370; -.
DR KEGG; sme:SMc03179; -.
DR PATRIC; fig|266834.11.peg.4431; -.
DR eggNOG; COG1009; Bacteria.
DR eggNOG; COG2111; Bacteria.
DR HOGENOM; CLU_007100_2_0_5; -.
DR OMA; AFILQYM; -.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005451; F:monovalent cation:proton antiporter activity; IEA:InterPro.
DR GO; GO:0098662; P:inorganic cation transmembrane transport; IEA:InterPro.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR005281; CPA3_sub_B.
DR InterPro; IPR025383; MbhD-like_TM.
DR InterPro; IPR007182; MnhB.
DR InterPro; IPR005663; MrpA/MnhA1/PhaAB.
DR InterPro; IPR001750; ND/Mrp_mem.
DR InterPro; IPR001516; Proton_antipo_N.
DR Pfam; PF13244; DUF4040; 1.
DR Pfam; PF04039; MnhB; 1.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR Pfam; PF00662; Proton_antipo_N; 1.
DR TIGRFAMs; TIGR00940; 2a6301s01; 1.
DR TIGRFAMs; TIGR00943; 2a6301s02; 1.
PE 3: Inferred from homology;
KW Antiport; Cell membrane; Hydrogen ion transport; Ion transport; Membrane;
KW Potassium; Potassium transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..999
FT /note="Probable K(+)/H(+) antiporter subunit A/B"
FT /id="PRO_0000217075"
FT TRANSMEM 31..48
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..160
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..255
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 389..411
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..464
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 488..510
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 530..552
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 604..621
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 636..653
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 660..682
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 686..708
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 729..751
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 788..807
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 846..868
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 878..900
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 913..935
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 955..977
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 122
FT /note="A -> V (in Ref. 1; CAA63734)"
FT /evidence="ECO:0000305"
FT CONFLICT 188
FT /note="G -> A (in Ref. 1; CAA63734)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 999 AA; 108790 MW; 9857B829580C2286 CRC64;
MTRPASVLAG PKSRPPIHSQ GDKTIEYAEK LLSVFILVPF AGSLIAIFFP SDQRGAISWF
AGAIALVCFL VTAGLYPYVA SGGVLHYRID WVPELGLNFT LRMDGFAWLF SALITAIGVL
VALYARYYMA EEDPVPRFFA LFLAFMGSML GVVLSGNLIL LAVFWELTSI VSFLLIGYWH
HNAHARDGAR MALTITGTGG LAMFVGLIII GKIVGSYELD AVLASGDAIR NHPLYGTVLV
LVLLGALTKS AQFPFHFWLP HAMAAPTPVS AYLHSATMVK AGVFLLVRFW PVMAGTEAWF
WIVGLAGLTT LLLGAYFAIF QQDLKGLLAY STISHLGLIT VLLSLGSPLA AVAAVFHIVN
HATFKASLFM AAGIIDHESG TRDIRRLGGL FHFMPITATL AMVASAAMAG VPLLNGFLSK
EMFFAEAIET HLVNPLDTVT PYVATIAGMF AVTYSLRFIH GVFFGRPPAD LPRKPHEPPR
WMRAPLDFLV LACLVVGIIP AQTIGPFLHT AVLSVLREGT PDYSLSVWHG WNIPLIMSFV
ALSGGIGLYF LMRSYLATAV EGPPVFRLLQ GQRIFERVLV TLSWKWARWL EQRLGTRRLQ
PQMRLLVFLA LAAGASPLLL GNFELPPLVI RGIDPAFALL WAIGIACAIG SAYQAKFHRL
ASLVLLGGAG LVTCITFVWL SAPDLAVTQL LVEIVTTVLI LLGLRWLPKR IEEPVAAEDI
SIRVRLRRLR DLLLAIGAGG GMMLIAYTVM TRPLPETIAS YFLERAYREG GGTNVVNVIL
VDFRGFDTLG EIAVLCIVAL TVFALLLRFR PQSDSLEAPE QQKVQNAFDD DHPDRAAGDS
VAEYLFIPAV IMRWMFPVTG MLAAFLFLRG HDLPGGGFAA GIAMSIGFIL QYMSGGTRWV
EERLRIHPLR WMSIGLLVAT ATGVGSWFFG YPFLTSHAQY ASLPVVGKFP LASAILFDLG
VFSLVLGATV LILIALAHQS VRAPRAHAKA ARSDKEAVR