PHAC_CHRVO
ID PHAC_CHRVO Reviewed; 568 AA.
AC Q9ZHI2;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 24-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Poly(3-hydroxyalkanoate) polymerase;
DE Short=PHA polymerase;
DE EC=2.3.1.-;
DE AltName: Full=PHA synthase;
DE AltName: Full=Polyhydroxyalkanoic acid synthase;
GN Name=phaC; OrderedLocusNames=CV_2789;
OS Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC
OS 12614 / NCIMB 9131 / NCTC 9757).
OC Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales;
OC Chromobacteriaceae; Chromobacterium.
OX NCBI_TaxID=243365;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC
RC 9757;
RX PubMed=10427049; DOI=10.1128/aem.65.8.3561-3565.1999;
RA Kolibachuk D., Miller A., Dennis D.;
RT "Cloning, molecular analysis, and expression of the polyhydroxyalkanoic
RT acid synthase (phaC) gene from Chromobacterium violaceum.";
RL Appl. Environ. Microbiol. 65:3561-3565(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC
RC 9757;
RX PubMed=14500782; DOI=10.1073/pnas.1832124100;
RA Vasconcelos A.T.R., de Almeida D.F., Hungria M., Guimaraes C.T.,
RA Antonio R.V., Almeida F.C., de Almeida L.G.P., de Almeida R.,
RA Alves-Gomes J.A., Andrade E.M., Araripe J., de Araujo M.F.F.,
RA Astolfi-Filho S., Azevedo V., Baptista A.J., Bataus L.A.M., Batista J.S.,
RA Belo A., van den Berg C., Bogo M., Bonatto S., Bordignon J., Brigido M.M.,
RA Brito C.A., Brocchi M., Burity H.A., Camargo A.A., Cardoso D.D.P.,
RA Carneiro N.P., Carraro D.M., Carvalho C.M.B., Cascardo J.C.M., Cavada B.S.,
RA Chueire L.M.O., Creczynski-Pasa T.B., Cunha-Junior N.C., Fagundes N.,
RA Falcao C.L., Fantinatti F., Farias I.P., Felipe M.S.S., Ferrari L.P.,
RA Ferro J.A., Ferro M.I.T., Franco G.R., Freitas N.S.A., Furlan L.R.,
RA Gazzinelli R.T., Gomes E.A., Goncalves P.R., Grangeiro T.B.,
RA Grattapaglia D., Grisard E.C., Hanna E.S., Jardim S.N., Laurino J.,
RA Leoi L.C.T., Lima L.F.A., Loureiro M.F., Lyra M.C.C.P., Madeira H.M.F.,
RA Manfio G.P., Maranhao A.Q., Martins W.S., di Mauro S.M.Z.,
RA de Medeiros S.R.B., Meissner R.V., Moreira M.A.M., Nascimento F.F.,
RA Nicolas M.F., Oliveira J.G., Oliveira S.C., Paixao R.F.C., Parente J.A.,
RA Pedrosa F.O., Pena S.D.J., Pereira J.O., Pereira M., Pinto L.S.R.C.,
RA Pinto L.S., Porto J.I.R., Potrich D.P., Ramalho-Neto C.E., Reis A.M.M.,
RA Rigo L.U., Rondinelli E., Santos E.B.P., Santos F.R., Schneider M.P.C.,
RA Seuanez H.N., Silva A.M.R., da Silva A.L.C., Silva D.W., Silva R.,
RA Simoes I.C., Simon D., Soares C.M.A., Soares R.B.A., Souza E.M.,
RA Souza K.R.L., Souza R.C., Steffens M.B.R., Steindel M., Teixeira S.R.,
RA Urmenyi T., Vettore A., Wassem R., Zaha A., Simpson A.J.G.;
RT "The complete genome sequence of Chromobacterium violaceum reveals
RT remarkable and exploitable bacterial adaptability.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:11660-11665(2003).
CC -!- FUNCTION: Involved in the production of polyhydroxyalkonic acids
CC (PHAs), composed primarily of 3-hydroxybutyric acid (3HB) and 3-
CC hydroxyvaleric acid (3HV).
CC -!- PATHWAY: Biopolymer metabolism; poly-(R)-3-hydroxybutanoate
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PHA/PHB synthase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF061446; AAC69615.1; -; Genomic_DNA.
DR EMBL; AE016825; AAQ60457.2; -; Genomic_DNA.
DR AlphaFoldDB; Q9ZHI2; -.
DR SMR; Q9ZHI2; -.
DR STRING; 243365.CV_2789; -.
DR ESTHER; chrvi-PHAC; PHA_synth_I.
DR EnsemblBacteria; AAQ60457; AAQ60457; CV_2789.
DR KEGG; cvi:CV_2789; -.
DR eggNOG; COG3243; Bacteria.
DR HOGENOM; CLU_017387_1_0_4; -.
DR OMA; YIYGSRE; -.
DR BRENDA; 2.3.1.304; 1370.
DR UniPathway; UPA00917; -.
DR Proteomes; UP000001424; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0042621; P:poly(3-hydroxyalkanoate) biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0042619; P:poly-hydroxybutyrate biosynthetic process; IEA:InterPro.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR010963; PHA_synth_I.
DR InterPro; IPR010941; PhaC_N.
DR Pfam; PF07167; PhaC_N; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
DR TIGRFAMs; TIGR01838; PHA_synth_I; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Cytoplasm; PHA biosynthesis; Reference proteome;
KW Transferase.
FT CHAIN 1..568
FT /note="Poly(3-hydroxyalkanoate) polymerase"
FT /id="PRO_0000215464"
FT ACT_SITE 292
FT /evidence="ECO:0000255"
FT CONFLICT 1..25
FT /note="MQQFVNSLSQPPAPDGAAHPFAGAW -> MDAAVRQLAVAASSPRTAQPIPS
FT PAL (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 41..56
FT /note="SLYQQQLNLWSQFLGQ -> GLPAAGQSVVAIGRP (in Ref. 1;
FT AAC69615)"
FT /evidence="ECO:0000305"
FT CONFLICT 89
FT /note="L -> V (in Ref. 1; AAC69615)"
FT /evidence="ECO:0000305"
FT CONFLICT 124
FT /note="Y -> F (in Ref. 1; AAC69615)"
FT /evidence="ECO:0000305"
FT CONFLICT 127
FT /note="A -> S (in Ref. 1; AAC69615)"
FT /evidence="ECO:0000305"
FT CONFLICT 524
FT /note="W -> M (in Ref. 1; AAC69615)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 568 AA; 63270 MW; BF83B10D0FC8C4BA CRC64;
MQQFVNSLSQ PPAPDGAAHP FAGAWAQLMN QTNQLFALQS SLYQQQLNLW SQFLGQAAGQ
EAAAEAGAKP ADRRFASPEW NEHPFYNFLK QSYLQTSKWM MELVDKTQLD EDAKDKLAFA
TRQYLDAMSP SNFMLTNPDV VKRAIETKGE SLVEGMKNML DDFQKGHISM SDESKFEIGK
NLVVTPGQVV FRNELIELIQ YTPTTDKVYE KPLLFVPPCI NKYYLMDLQP DNSMVRHFVA
QGYRVFLISW RSAVAEMKHF TWETYIEKGV FAAAEAVQKI TKQPTMNVLG FCVGGVILTT
ALCVAQAKGL KYFDSATFMT SLIDHAEPGE ISFFIDESVV AGREAKMASG GIISGKEIGR
TFASLRANDL VWNYVVNNYL LGKTPAPFDL LFWNNDAVDL PLPMHTFLLR QFYMNNALVR
PGAITLCGVP IDIAKIDVPV YMFAARDDHI VLWSSAFSGL KYLQGAPSRR FVLGASGHIA
GSINPVTKDK RNYWANDTLP LHAEEWLESA ESRPGSWWKD WDAWLAPQSG KQVAAPKSLG
NKEFPPLLAA PGSYVLAKAM PSVAASLQ
