ID   PHAC_METEX              Reviewed;         605 AA.
AC   P52070;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Poly(3-hydroxyalkanoate) polymerase;
DE            Short=PHA polymerase;
DE            EC=2.3.1.-;
DE   AltName: Full=PHA synthase;
DE   AltName: Full=Polyhydroxyalkanoic acid synthase;
GN   Name=phaC;
OS   Methylorubrum extorquens (Methylobacterium dichloromethanicum)
OS   (Methylobacterium extorquens).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylorubrum.
OX   NCBI_TaxID=408;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=IBT 6;
RX   PubMed=7763712; DOI=10.1007/bf00192084;
RA   Valentin H.E., Steinbuechel A.;
RT   "Cloning and characterization of the Methylobacterium extorquens
RT   polyhydroxyalkanoic-acid-synthase structural gene.";
RL   Appl. Microbiol. Biotechnol. 39:309-317(1993).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PHA/PHB synthase family. {ECO:0000305}.
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DR   EMBL; L07893; AAA72330.1; -; Genomic_DNA.
DR   AlphaFoldDB; P52070; -.
DR   SMR; P52070; -.
DR   ESTHER; metex-phac; PHA_synth_I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0042621; P:poly(3-hydroxyalkanoate) biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0042619; P:poly-hydroxybutyrate biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR010963; PHA_synth_I.
DR   InterPro; IPR010941; PhaC_N.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   Pfam; PF07167; PhaC_N; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
DR   TIGRFAMs; TIGR01838; PHA_synth_I; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Cytoplasm; PHA biosynthesis; Transferase.
FT   CHAIN           1..605
FT                   /note="Poly(3-hydroxyalkanoate) polymerase"
FT                   /id="PRO_0000215467"
FT   DOMAIN          319..527
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        341
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   605 AA;  66773 MW;  624F515F367809CD CRC64;
     MGTERTNPAA PDFETIARNA NQLAEVFRQS AAASLKPFEP AGQGALLPGA NLQGASEIDE
     MTRTLTRVAE TWLKDPEKAL QAQTKLGQSF AALWASTLTR MQGAVTEPVV QPPPTDKRFA
     HADWSANPVF DLIKQSYLLL GRWAEEMVET AEGIDEHTRH KAEFYLRQLL SAYSPSNFVM
     TNPELLRQTL EEGGANLMRG MKMLQEDLEA GGGQLRVRQT DLSAFTFGKD VAVTPGEVIF
     RNDLMELIQY APTTETVLKR PLLIVPPWIN KFYILDLNPQ KSLIGWMVSQ GITVFVISWV
     NPDERHRDKD FESYMREGIE TAIDMIGVAT GETDVAAAGY CVGGTLLAVT LAYQAATGNR
     RIKSATFLTT QVDFTHAGDL KVFADEGQIK AIEERMAEHG YLEGARMANA FNMLRPNDLI
     WSYVVNNYVR GKAPAAFDLL YWNADATRMP AANHSFYLRN CYLNNTLAKG QMVLGNVRLD
     LKKVKVPVFN LATREDHIAP ALSVFEGSAK FGGKVDYVLA GSGHIAGVVA PPGPKAKYGF
     RTGGPARGRF EDWVAAATEH PGSWWPYWYK WLEEQAPERV PARIPGTGAL PSLAPAPGTY
     VRMKA