PHAD_RHIME
ID PHAD_RHIME Reviewed; 539 AA.
AC Q52981; Q92LV6;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Probable K(+)/H(+) antiporter subunit D;
DE AltName: Full=pH adaptation potassium efflux system protein D;
DE Short=Pha system subunit D;
GN Name=phaD; Synonyms=phaD1; OrderedLocusNames=R02912; ORFNames=SMc03181;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=41;
RX PubMed=9680201; DOI=10.1046/j.1365-2958.1998.00868.x;
RA Putnoky P., Kereszt A., Nakamura T., Endre G., Grosskopf E., Kiss P.,
RA Kondorosi A.;
RT "The pha gene cluster of Rhizobium meliloti involved in pH adaptation and
RT symbiosis encodes a novel type of K+ efflux system.";
RL Mol. Microbiol. 28:1091-1101(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- FUNCTION: Part of a K(+) efflux system which is required for the
CC adaptation of R.meliloti to alkaline pH as well as for the infection
CC process during symbiotic nodule development.
CC -!- SUBUNIT: May form a hetero-oligomeric complex that consists of six
CC subunits: PhaAB, PhaC, PhaD, PhaE, PhaF and PhaG.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA63737.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X93358; CAA63737.1; ALT_INIT; Genomic_DNA.
DR EMBL; AL591688; CAC47491.1; -; Genomic_DNA.
DR RefSeq; NP_387018.1; NC_003047.1.
DR RefSeq; WP_013844865.1; NC_003047.1.
DR AlphaFoldDB; Q52981; -.
DR SMR; Q52981; -.
DR STRING; 266834.SMc03181; -.
DR TCDB; 2.A.63.1.1; the monovalent cation (k(+) or na(+)):proton antiporter-3 (cpa3) family.
DR EnsemblBacteria; CAC47491; CAC47491; SMc03181.
DR GeneID; 61604372; -.
DR KEGG; sme:SMc03181; -.
DR PATRIC; fig|266834.11.peg.4433; -.
DR eggNOG; COG0651; Bacteria.
DR HOGENOM; CLU_007100_9_2_5; -.
DR OMA; YVAHHIT; -.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015386; F:potassium:proton antiporter activity; IEA:InterPro.
DR InterPro; IPR004775; MnhD1.
DR InterPro; IPR001750; ND/Mrp_mem.
DR Pfam; PF00361; Proton_antipo_M; 1.
DR TIGRFAMs; TIGR00944; 2a6301s04; 1.
PE 3: Inferred from homology;
KW Antiport; Cell membrane; Hydrogen ion transport; Ion transport; Membrane;
KW Potassium; Potassium transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..539
FT /note="Probable K(+)/H(+) antiporter subunit D"
FT /id="PRO_0000217085"
FT TRANSMEM 4..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..58
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 78..100
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 175..197
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 283..305
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 335..357
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 400..422
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..464
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 484..506
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 539 AA; 56303 MW; 2EA3B3FA2D406722 CRC64;
MTDWLDHLLI LPILLPLAVA AVLIPINERD RTLKGAIGFA STLVVFILSM ILMRLAAAGT
GSLPGSGVYQ LGNWPAPFGI VLVLDRLSAL MLCLTSGLAL AAQAYSMARW HTAGHHFHSL
FQLLVAGLNG AFLTGDLFNL FVFFEMMLAA SYGLLLHGSG PLRVKAGLHY IAVNLAASAL
FLIGVSLIYG AAGTLNMADL ATKLAALEPR SRTLVEMGSA ILGVAFLVKA GMWPLSFWLP
TAYAAATPPV AGVFAVLTKV GIYVIIRLHL LVFGTAAGAS SGFGQEWLVT GGMLTIAFGG
IGVLASQAMG RLAGYSVLVS SGTLLAAVGL GHDGMLAGAL FYLVSSTLTI GAFFLLIELV
ERGRDAGADV LAVTMEAYGD FDEDEEEEEV GAAIPGTMAV LGLCFCLCAL LLAGLPPLSG
FIAKFALISG LFDMPAAELA TAMSAADWTY VTLLILSGLA AMIAMNRIGI RTFWASIEGT
IPRVVVIEIT PVVVLLGACI FLSLQAGPAM RYMQATADDL LAPLTHSERV LSAPRAGSQ
