ID   PHAE_HALHT              Reviewed;         181 AA.
AC   G0HQZ5; A8C9M3;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   19-OCT-2011, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=Poly(3-hydroxyalkanoate) polymerase subunit PhaE;
DE            Short=PHA polymerase;
DE            EC=2.3.1.-;
DE   AltName: Full=PHB synthase subunit PhaE;
DE   AltName: Full=Poly(3-hydroxybutyrate) polymerase subunit PhaE;
DE            Short=PHB polymerase;
DE   AltName: Full=Polyhydroxyalkanoic acid synthase subunit PhaE;
DE            Short=PHA synthase;
GN   Name=phaE; OrderedLocusNames=HAH_0153;
OS   Haloarcula hispanica (strain ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182
OS   / NCIMB 2187 / VKM B-1755).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Haloarculaceae; Haloarcula.
OX   NCBI_TaxID=634497;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182 / NCIMB 2187 / VKM
RC   B-1755;
RX   PubMed=17675423; DOI=10.1128/aem.00953-07;
RA   Han J., Lu Q., Zhou L., Zhou J., Xiang H.;
RT   "Molecular characterization of the phaECHm genes, required for biosynthesis
RT   of poly(3-hydroxybutyrate) in the extremely halophilic archaeon Haloarcula
RT   marismortui.";
RL   Appl. Environ. Microbiol. 73:6058-6065(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33960 / DSM 4426 / JCM 8911 / NBRC 102182 / NCIMB 2187 / VKM
RC   B-1755;
RX   PubMed=21994921; DOI=10.1128/jb.05953-11;
RA   Liu H., Wu Z., Li M., Zhang F., Zheng H., Han J., Liu J., Zhou J., Wang S.,
RA   Xiang H.;
RT   "Complete genome sequence of Haloarcula hispanica, a model haloarchaeon for
RT   studying genetics, metabolism, and virus-host interaction.";
RL   J. Bacteriol. 193:6086-6087(2011).
CC   -!- FUNCTION: Involved in the production of polyhydroxyalkonic acids
CC       (PHAs), which are water-insoluble biopolymers used as intracellular
CC       energy reserve material when cells grow under conditions of nutrient
CC       limitation. PHAs are composed primarily of 3-hydroxybutyric acid (3HB)
CC       and 3-hydroxyvaleric acid (3HV). Required for the production of poly-
CC       beta-hydroxybutyrate (PHB) and poly(beta-hydroxybutyrate-co-beta-
CC       hydroxyvalerate) (PHBV). {ECO:0000269|PubMed:17675423}.
CC   -!- PATHWAY: Biopolymer metabolism; poly-(R)-3-hydroxybutanoate
CC       biosynthesis.
CC   -!- SUBUNIT: Heterodimer with PhaC. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Depletion of both phaC and phaE genes leads to
CC       complete loss of the PHA synthase activity.
CC       {ECO:0000269|PubMed:17675423}.
CC   -!- BIOTECHNOLOGY: PHB and PHBV are desirable bioplastic due to their
CC       biodegradability, biocompatibility, and mechanical properties. However,
CC       PHBV has better mechanical properties than PHB.
CC   -!- SIMILARITY: Belongs to the PHA/PHB synthase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EU022705; ABV71393.1; -; Genomic_DNA.
DR   EMBL; CP002921; AEM55786.1; -; Genomic_DNA.
DR   RefSeq; WP_014039170.1; NC_015948.1.
DR   AlphaFoldDB; G0HQZ5; -.
DR   SMR; G0HQZ5; -.
DR   STRING; 634497.HAH_0153; -.
DR   EnsemblBacteria; AEM55786; AEM55786; HAH_0153.
DR   GeneID; 23803421; -.
DR   KEGG; hhi:HAH_0153; -.
DR   eggNOG; arCOG06343; Archaea.
DR   HOGENOM; CLU_1485849_0_0_2; -.
DR   OMA; EFRDIWL; -.
DR   OrthoDB; 83890at2157; -.
DR   UniPathway; UPA00917; -.
DR   Proteomes; UP000005629; Chromosome I.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0042621; P:poly(3-hydroxyalkanoate) biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0042619; P:poly-hydroxybutyrate biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR010123; PHA_synth_III_E.
DR   Pfam; PF09712; PHA_synth_III_E; 1.
PE   1: Evidence at protein level;
KW   Acyltransferase; PHA biosynthesis; PHB biosynthesis; Transferase.
FT   CHAIN           1..181
FT                   /note="Poly(3-hydroxyalkanoate) polymerase subunit PhaE"
FT                   /id="PRO_0000428872"
SQ   SEQUENCE   181 AA;  20610 MW;  61D59E6AEE9A9C00 CRC64;
     MSNTNNIQEE WTEMVEEMNN AVADSMEQNM KAQAAFVESW ADAVEDTIPK EEDLADGMDG
     YNRAYEEWID AAEQMVERST DAAQGEDVDP AEFRDIWLQS ANEAFKHVMG TSAFAAANGQ
     LVESMMEMQQ EADDLSQDAL EQMGFPTRND VDEVAERLIE LERRQHAVEQ KLDRVLEHLE
     E