ID   PHAE_HALMA              Reviewed;         181 AA.
AC   Q5UYM1;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Poly(3-hydroxyalkanoate) polymerase subunit PhaE;
DE            Short=PHA polymerase;
DE            EC=2.3.1.-;
DE   AltName: Full=PHB synthase;
DE   AltName: Full=Poly(3-hydroxybutyrate) polymerase subunit PhaE;
DE            Short=PHB polymerase;
DE   AltName: Full=Polyhydroxyalkanoic acid synthase subunit PhaE;
DE            Short=PHA synthase;
GN   Name=phaE; OrderedLocusNames=rrnAC2885;
OS   Haloarcula marismortui (strain ATCC 43049 / DSM 3752 / JCM 8966 / VKM
OS   B-1809) (Halobacterium marismortui).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Haloarculaceae; Haloarcula.
OX   NCBI_TaxID=272569;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809;
RX   PubMed=15520287; DOI=10.1101/gr.2700304;
RA   Baliga N.S., Bonneau R., Facciotti M.T., Pan M., Glusman G., Deutsch E.W.,
RA   Shannon P., Chiu Y., Weng R.S., Gan R.R., Hung P., Date S.V., Marcotte E.,
RA   Hood L., Ng W.V.;
RT   "Genome sequence of Haloarcula marismortui: a halophilic archaeon from the
RT   Dead Sea.";
RL   Genome Res. 14:2221-2234(2004).
RN   [2]
RP   FUNCTION.
RC   STRAIN=ATCC 43049 / DSM 3752 / JCM 8966 / VKM B-1809;
RX   PubMed=17675423; DOI=10.1128/aem.00953-07;
RA   Han J., Lu Q., Zhou L., Zhou J., Xiang H.;
RT   "Molecular characterization of the phaECHm genes, required for biosynthesis
RT   of poly(3-hydroxybutyrate) in the extremely halophilic archaeon Haloarcula
RT   marismortui.";
RL   Appl. Environ. Microbiol. 73:6058-6065(2007).
CC   -!- FUNCTION: Involved in the production of polyhydroxyalkonic acids
CC       (PHAs), which are water-insoluble biopolymers used as intracellular
CC       energy reserve material when cells grow under conditions of nutrient
CC       limitation. PHAs are composed primarily of 3-hydroxybutyric acid (3HB)
CC       and 3-hydroxyvaleric acid (3HV). Required for the production of poly-
CC       beta-hydroxybutyrate (PHB) and poly(beta-hydroxybutyrate-co-beta-
CC       hydroxyvalerate) (PHBV). {ECO:0000269|PubMed:17675423}.
CC   -!- PATHWAY: Biopolymer metabolism; poly-(R)-3-hydroxybutanoate
CC       biosynthesis.
CC   -!- SUBUNIT: Heterodimer with PhaC. {ECO:0000250}.
CC   -!- BIOTECHNOLOGY: PHB and PHBV are desirable bioplastic due to their
CC       biodegradability, biocompatibility, and mechanical properties. However,
CC       PHBV has better mechanical properties than PHB.
CC   -!- SIMILARITY: Belongs to the PHA/PHB synthase family. {ECO:0000305}.
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DR   EMBL; AY596297; AAV47632.1; -; Genomic_DNA.
DR   RefSeq; WP_004960317.1; NZ_CP039138.1.
DR   AlphaFoldDB; Q5UYM1; -.
DR   SMR; Q5UYM1; -.
DR   STRING; 272569.rrnAC2885; -.
DR   EnsemblBacteria; AAV47632; AAV47632; rrnAC2885.
DR   GeneID; 40153728; -.
DR   GeneID; 64823906; -.
DR   KEGG; hma:rrnAC2885; -.
DR   PATRIC; fig|272569.17.peg.3453; -.
DR   eggNOG; arCOG06343; Archaea.
DR   HOGENOM; CLU_1485849_0_0_2; -.
DR   OMA; EFRDIWL; -.
DR   UniPathway; UPA00917; -.
DR   Proteomes; UP000001169; Chromosome I.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0042621; P:poly(3-hydroxyalkanoate) biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0042619; P:poly-hydroxybutyrate biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR010123; PHA_synth_III_E.
DR   Pfam; PF09712; PHA_synth_III_E; 1.
PE   1: Evidence at protein level;
KW   Acyltransferase; PHA biosynthesis; PHB biosynthesis; Reference proteome;
KW   Transferase.
FT   CHAIN           1..181
FT                   /note="Poly(3-hydroxyalkanoate) polymerase subunit PhaE"
FT                   /id="PRO_0000428873"
SQ   SEQUENCE   181 AA;  20640 MW;  2395B62F491A61DB CRC64;
     MSNTNNIQEE WTEMVEEMNN AVADSMEQNM KAQAAFVESW ADAVEDTIPE QDDLADGMDG
     YNRAYEEWMD AAEQMVERST DAAQGEDVDP AEFRDIWLQS ANEAFKHVMG TSAFAAANGQ
     LVESMMEMQQ EADDLSQDTL EQLGFPTRND VDEIAERLIE LERRQHAVEQ KLDRVLEHLE
     E