ID   PHAF1_CAEEL             Reviewed;         422 AA.
AC   P34692; Q22076; Q9U383;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2002, sequence version 3.
DT   03-AUG-2022, entry version 143.
DE   RecName: Full=PHAF1 protein T01G9.2;
GN   ORFNames=T01G9.2;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
RC   STRAIN=Bristol N2;
RX   PubMed=2159938; DOI=10.1101/gad.4.3.357;
RA   Kim S.K., Horvitz H.R.;
RT   "The Caenorhabditis elegans gene lin-10 is broadly expressed while required
RT   specifically for the determination of vulval cell fates.";
RL   Genes Dev. 4:357-371(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
CC   -!- FUNCTION: May play a regulatory role in autophagic activity.
CC       {ECO:0000250|UniProtKB:Q9BSU1}.
CC   -!- INTERACTION:
CC       P34692; Q9UAT1: CELE_D2063.1; NbExp=3; IntAct=EBI-326630, EBI-325518;
CC       P34692; G5EFF2: CELE_F20C5.6; NbExp=3; IntAct=EBI-326630, EBI-320916;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BSU1}.
CC       Preautophagosomal structure {ECO:0000250|UniProtKB:Q9BSU1}. Note=The
CC       BCAS3:PHAF1 complex is recruited to the preautophagosomal structures
CC       adjacent to the damaged mitochondria upon mitophagy in a PRKN-PINK1
CC       dependent manner. {ECO:0000250|UniProtKB:Q9BSU1}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=a;
CC         IsoId=P34692-1; Sequence=Displayed;
CC       Name=b;
CC         IsoId=P34692-2; Sequence=VSP_006725;
CC   -!- SIMILARITY: Belongs to the PHAF1 family. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to be lin-10.
CC       {ECO:0000305|PubMed:2159938}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA35704.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; X51321; CAA35704.1; ALT_INIT; mRNA.
DR   EMBL; Z75713; CAB00051.2; -; Genomic_DNA.
DR   EMBL; Z75713; CAB54301.1; -; Genomic_DNA.
DR   PIR; A34589; A34589.
DR   PIR; T24321; T24321.
DR   PIR; T24322; T24322.
DR   RefSeq; NP_492252.1; NM_059851.3. [P34692-2]
DR   RefSeq; NP_740894.1; NM_171841.6. [P34692-1]
DR   AlphaFoldDB; P34692; -.
DR   BioGRID; 38043; 9.
DR   IntAct; P34692; 5.
DR   STRING; 6239.T01G9.2a; -.
DR   iPTMnet; P34692; -.
DR   EPD; P34692; -.
DR   PaxDb; P34692; -.
DR   PeptideAtlas; P34692; -.
DR   EnsemblMetazoa; T01G9.2a.1; T01G9.2a.1; WBGene00011344. [P34692-1]
DR   EnsemblMetazoa; T01G9.2b.1; T01G9.2b.1; WBGene00011344. [P34692-2]
DR   GeneID; 172609; -.
DR   KEGG; cel:CELE_T01G9.2; -.
DR   UCSC; T01G9.2b.1; c. elegans. [P34692-1]
DR   CTD; 172609; -.
DR   WormBase; T01G9.2a; CE27202; WBGene00011344; -. [P34692-1]
DR   WormBase; T01G9.2b; CE23937; WBGene00011344; -. [P34692-2]
DR   eggNOG; KOG2819; Eukaryota.
DR   GeneTree; ENSGT00940000153528; -.
DR   InParanoid; P34692; -.
DR   OMA; GCEQWEF; -.
DR   OrthoDB; 709631at2759; -.
DR   PhylomeDB; P34692; -.
DR   PRO; PR:P34692; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00011344; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR   GO; GO:0005737; C:cytoplasm; HDA:WormBase.
DR   GO; GO:0000407; C:phagophore assembly site; ISS:UniProtKB.
DR   GO; GO:0055120; C:striated muscle dense body; HDA:WormBase.
DR   GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR   GO; GO:0043001; P:Golgi to plasma membrane protein transport; IBA:GO_Central.
DR   InterPro; IPR005373; PHAF1.
DR   InterPro; IPR039156; PHAF1/BROMI.
DR   PANTHER; PTHR13465; PTHR13465; 1.
DR   PANTHER; PTHR13465:SF2; PTHR13465:SF2; 1.
DR   Pfam; PF03676; UPF0183; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Cytoplasm; Reference proteome.
FT   CHAIN           1..422
FT                   /note="PHAF1 protein T01G9.2"
FT                   /id="PRO_0000221086"
FT   VAR_SEQ         21..23
FT                   /note="Missing (in isoform b)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_006725"
FT   CONFLICT        1..6
FT                   /note="MNGKQQ -> MMQTA (in Ref. 1; CAA35704)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   422 AA;  48211 MW;  2DFC14A9A652F571 CRC64;
     MNGKQQVTKK PSTEEDLSFT KFKVVPDVGL KSSQFEFVLG MPINQCIAMI QQHPRMLTKV
     ELKYSKKDPF YQDIIIYIGS TGIRLYFDGL SQLIKLIEVD NLSMITLTYN DTIFSDPNNM
     ATLDRVNEFF GSTHPGSYDD KHNIYVQSWP GLSFCFPYGG ENSNLEVRPG FGGNLRSLKY
     DANSQPKLTK MSIYRGPNPS EPESVDTPFS CYCGQNRTRK VEAIWENGNI VGIDIQFDTQ
     NGRIVDGEYD VSTYTRQIYF GDSVSDVQSI LGAPTKVFYK SDDKMKIHRG LHKETLYGPP
     NFFFNYFVMG LDILFDFVSK RVVKFVLHTN APGHCDFGMY SRCNFSIFLN DKQYEIRTDS
     KFDEFSHAFM NDSNPPRPVV LARQEQQPFG STFCYGIKQI IVERTENGFL TSVTIYDGAK
     EK