PHAF1_DROME
ID PHAF1_DROME Reviewed; 438 AA.
AC Q9VSH9; A8WHJ9;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=PHAF1 protein CG7083;
GN ORFNames=CG7083;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May play a regulatory role in autophagic activity.
CC {ECO:0000250|UniProtKB:Q9BSU1}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BSU1}.
CC Preautophagosomal structure {ECO:0000250|UniProtKB:Q9BSU1}. Note=The
CC BCAS3:PHAF1 complex is recruited to the preautophagosomal structures
CC adjacent to the damaged mitochondria upon mitophagy in a PRKN-PINK1
CC dependent manner. {ECO:0000250|UniProtKB:Q9BSU1}.
CC -!- SIMILARITY: Belongs to the PHAF1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014296; AAF50440.1; -; Genomic_DNA.
DR EMBL; BT031145; ABX00767.1; -; mRNA.
DR RefSeq; NP_648211.1; NM_139954.2.
DR AlphaFoldDB; Q9VSH9; -.
DR STRING; 7227.FBpp0076331; -.
DR PaxDb; Q9VSH9; -.
DR PRIDE; Q9VSH9; -.
DR DNASU; 38943; -.
DR EnsemblMetazoa; FBtr0076604; FBpp0076331; FBgn0035877.
DR GeneID; 38943; -.
DR KEGG; dme:Dmel_CG7083; -.
DR UCSC; CG7083-RA; d. melanogaster.
DR FlyBase; FBgn0035877; CG7083.
DR VEuPathDB; VectorBase:FBgn0035877; -.
DR eggNOG; KOG2819; Eukaryota.
DR GeneTree; ENSGT00940000153528; -.
DR HOGENOM; CLU_032056_0_0_1; -.
DR InParanoid; Q9VSH9; -.
DR OMA; GCEQWEF; -.
DR OrthoDB; 709631at2759; -.
DR PhylomeDB; Q9VSH9; -.
DR BioGRID-ORCS; 38943; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 38943; -.
DR PRO; PR:Q9VSH9; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0035877; Expressed in oviduct (Drosophila) and 29 other tissues.
DR Genevisible; Q9VSH9; DM.
DR GO; GO:0000407; C:phagophore assembly site; ISS:UniProtKB.
DR GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR GO; GO:0043001; P:Golgi to plasma membrane protein transport; IBA:GO_Central.
DR InterPro; IPR005373; PHAF1.
DR InterPro; IPR039156; PHAF1/BROMI.
DR PANTHER; PTHR13465; PTHR13465; 1.
DR PANTHER; PTHR13465:SF2; PTHR13465:SF2; 1.
DR Pfam; PF03676; UPF0183; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Reference proteome.
FT CHAIN 1..438
FT /note="PHAF1 protein CG7083"
FT /id="PRO_0000221087"
SQ SEQUENCE 438 AA; 49264 MW; 2AF709AD30804AA5 CRC64;
MLDLEIVPEI SLGCDAWEFV LGMHFSQAIA IIQSQVGIIK GVQVLYSDTT PLGVDIIINL
PQDGVRLIFD PVSQRLKTIE VFNMKLVKLR YFGVYFNSPE VLPSIEQIEH SFGATHPGVY
DAAKQLFALH FRGLSFYFPV DSKLHSGYAH GLSSLVFLNG ASPVVSKMSL YAGSNVLENR
VPSLPLSCYH RQMYLESATV LRTAFGHTKG LKLKLFTEGS GRALEPRRQC FTRELLFGDS
CEDVATSLGA PNRIFFKSED KMKIHSSSVN RQAQSKRSDI FFNYFTLGID VLFDARTQTC
KKFILHTNYP GHFNFNMYHR CEFQFLLQAD HPSMSDSGHD LVTPTKQEHV NITAYTKWDA
ISSALATSER PVVLHRASST NTANPFGSTF CYGYQDLIFE VMPNSHIASV TLYNTAPPRQ
PAHSWQQHKM QDIRLTVA
