PHAF1_MOUSE
ID PHAF1_MOUSE Reviewed; 422 AA.
AC Q922R1; Q6PER2;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Phagosome assembly factor 1 {ECO:0000312|MGI:MGI:2443049};
GN Name=Phaf1 {ECO:0000312|MGI:MGI:2443049};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD; TISSUE=Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a regulatory role in autophagic activity. In complex
CC with BCAS3, associates with the autophagosome formation site during
CC both non-selective and selective autophagy.
CC {ECO:0000250|UniProtKB:Q9BSU1}.
CC -!- SUBUNIT: Interacts with BCAS3; the interaction is requrired for the
CC association with the phagophore. {ECO:0000250|UniProtKB:Q9BSU1}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BSU1}.
CC Preautophagosomal structure {ECO:0000250|UniProtKB:Q9BSU1}. Note=The
CC BCAS3:PHAF1 complex is recruited to the preautophagosomal structures
CC adjacent to the damaged mitochondria upon mitophagy in a PRKN-PINK1
CC dependent manner. {ECO:0000250|UniProtKB:Q9BSU1}.
CC -!- SIMILARITY: Belongs to the PHAF1 family. {ECO:0000305}.
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DR EMBL; AK156120; BAE33592.1; -; mRNA.
DR EMBL; CH466525; EDL11253.1; -; Genomic_DNA.
DR EMBL; BC006874; AAH06874.1; -; mRNA.
DR EMBL; BC057919; AAH57919.1; -; mRNA.
DR CCDS; CCDS22593.1; -.
DR RefSeq; NP_663579.2; NM_145604.2.
DR AlphaFoldDB; Q922R1; -.
DR CORUM; Q922R1; -.
DR STRING; 10090.ENSMUSP00000034361; -.
DR iPTMnet; Q922R1; -.
DR PhosphoSitePlus; Q922R1; -.
DR EPD; Q922R1; -.
DR MaxQB; Q922R1; -.
DR PaxDb; Q922R1; -.
DR PeptideAtlas; Q922R1; -.
DR PRIDE; Q922R1; -.
DR Antibodypedia; 29405; 46 antibodies from 19 providers.
DR DNASU; 234678; -.
DR Ensembl; ENSMUST00000034361; ENSMUSP00000034361; ENSMUSG00000031889.
DR GeneID; 234678; -.
DR KEGG; mmu:234678; -.
DR UCSC; uc009nbt.2; mouse.
DR CTD; 80262; -.
DR MGI; MGI:2443049; Phaf1.
DR VEuPathDB; HostDB:ENSMUSG00000031889; -.
DR eggNOG; KOG2819; Eukaryota.
DR GeneTree; ENSGT00940000153528; -.
DR HOGENOM; CLU_032056_0_1_1; -.
DR InParanoid; Q922R1; -.
DR OMA; GCEQWEF; -.
DR OrthoDB; 709631at2759; -.
DR PhylomeDB; Q922R1; -.
DR TreeFam; TF313669; -.
DR BioGRID-ORCS; 234678; 3 hits in 71 CRISPR screens.
DR PRO; PR:Q922R1; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q922R1; protein.
DR Bgee; ENSMUSG00000031889; Expressed in cleaving embryo and 249 other tissues.
DR ExpressionAtlas; Q922R1; baseline and differential.
DR Genevisible; Q922R1; MM.
DR GO; GO:0030425; C:dendrite; ISO:MGI.
DR GO; GO:0016020; C:membrane; ISO:MGI.
DR GO; GO:0000407; C:phagophore assembly site; ISS:UniProtKB.
DR GO; GO:0030672; C:synaptic vesicle membrane; ISO:MGI.
DR GO; GO:0005802; C:trans-Golgi network; ISO:MGI.
DR GO; GO:0035254; F:glutamate receptor binding; ISO:MGI.
DR GO; GO:0030165; F:PDZ domain binding; ISO:MGI.
DR GO; GO:0043001; P:Golgi to plasma membrane protein transport; ISO:MGI.
DR InterPro; IPR005373; PHAF1.
DR InterPro; IPR039156; PHAF1/BROMI.
DR PANTHER; PTHR13465; PTHR13465; 1.
DR PANTHER; PTHR13465:SF2; PTHR13465:SF2; 1.
DR Pfam; PF03676; UPF0183; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Reference proteome.
FT CHAIN 1..422
FT /note="Phagosome assembly factor 1"
FT /id="PRO_0000221084"
FT CONFLICT 356
FT /note="E -> D (in Ref. 3; AAH06874)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 422 AA; 47416 MW; 5639E2BB96D8DEA4 CRC64;
MLDLEVVPER SLGNEQWEFT LGMPLAQAVA ILQKHCRIIR NVQVLYSEQS PLSHDLILNL
TQDGITLLFD AFNQRLKVIE VCELTKVKLK YCGVHFNSQA IAPTIEQIDQ SFGATHPGVY
NSTEQLFHLN FRGLSFSFQL DSWTEAPKYE PNFAHGLASL QIPHGATVKR MYIYSGNSLQ
DTKAPVMPLS CFLGNVYAES VDVLRDGTGP SGLRLRLLAA GCGPGVLADA KMRVFERAVY
FGDSCQDVLS MLGSPHKVFY KSEDKMKIHS PSPHKQVPSK CNDYFFNYFT LGVDILFDAN
THKVKKFVLH TNYPGHYNFN IYHRCEFKIP LAIKKENAGG QTEICTTYSK WDSIQELLGH
PVEKPVVLHR SSSPNNTNPF GSTFCFGLQR MIFEVMQNNH IASVTLYGPP RPGAHLRTAE
LP
