PHAF_RHIME
ID PHAF_RHIME Reviewed; 92 AA.
AC Q52983;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 103.
DE RecName: Full=Probable K(+)/H(+) antiporter subunit F;
DE AltName: Full=pH adaptation potassium efflux system protein F;
DE Short=Pha system subunit F;
GN Name=phaF; Synonyms=phaF1; OrderedLocusNames=R02914; ORFNames=SMc03183;
OS Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium
OS meliloti).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=266834;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=41;
RX PubMed=9680201; DOI=10.1046/j.1365-2958.1998.00868.x;
RA Putnoky P., Kereszt A., Nakamura T., Endre G., Grosskopf E., Kiss P.,
RA Kondorosi A.;
RT "The pha gene cluster of Rhizobium meliloti involved in pH adaptation and
RT symbiosis encodes a novel type of K+ efflux system.";
RL Mol. Microbiol. 28:1091-1101(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11481430; DOI=10.1073/pnas.161294398;
RA Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J.,
RA Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S.,
RA Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T.,
RA Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C.,
RA Thebault P., Vandenbol M., Weidner S., Galibert F.;
RT "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium
RT meliloti strain 1021.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1021;
RX PubMed=11474104; DOI=10.1126/science.1060966;
RA Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F.,
RA Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G.,
RA Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P.,
RA Cowie A., Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S.,
RA Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I.,
RA Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S.,
RA Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C.,
RA Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R.,
RA Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H.,
RA Wong K., Yeh K.-C., Batut J.;
RT "The composite genome of the legume symbiont Sinorhizobium meliloti.";
RL Science 293:668-672(2001).
CC -!- FUNCTION: Part of a K(+) efflux system which is required for the
CC adaptation of R.meliloti to alkaline pH as well as for the infection
CC process during symbiotic nodule development.
CC -!- SUBUNIT: May form a hetero-oligomeric complex that consists of six
CC subunits: PhaAB, PhaC, PhaD, PhaE, PhaF and PhaG.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the CPA3 antiporters (TC 2.A.63) subunit F
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAC47493.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X93358; CAA63739.1; -; Genomic_DNA.
DR EMBL; AL591688; CAC47493.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_387020.1; NC_003047.1.
DR AlphaFoldDB; Q52983; -.
DR SMR; Q52983; -.
DR STRING; 266834.SMc03183; -.
DR TCDB; 2.A.63.1.1; the monovalent cation (k(+) or na(+)):proton antiporter-3 (cpa3) family.
DR EnsemblBacteria; CAC47493; CAC47493; SMc03183.
DR KEGG; sme:SMc03183; -.
DR PATRIC; fig|266834.11.peg.4435; -.
DR eggNOG; COG2212; Bacteria.
DR HOGENOM; CLU_125825_1_3_5; -.
DR OMA; VIAFARY; -.
DR Proteomes; UP000001976; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015075; F:ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR007208; MrpF/PhaF-like.
DR PANTHER; PTHR34702; PTHR34702; 1.
DR Pfam; PF04066; MrpF_PhaF; 1.
DR PIRSF; PIRSF028784; MrpF; 1.
PE 3: Inferred from homology;
KW Antiport; Cell membrane; Hydrogen ion transport; Ion transport; Membrane;
KW Potassium; Potassium transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..92
FT /note="Probable K(+)/H(+) antiporter subunit F"
FT /id="PRO_0000087744"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 92 AA; 10144 MW; 071ADF4CB950B0A8 CRC64;
MELAVVWSVL VAQTMLALAM AFALYRMARG PRAQDRILGL DTLYINAMLM LITFGIRTAN
TVYFETALII AVIGFASSIA LAKFLMRGEV IE
