ID   PHAP_HALMT              Reviewed;         154 AA.
AC   I3R9Z2; B3FRM3;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2012, sequence version 1.
DT   25-MAY-2022, entry version 37.
DE   RecName: Full=PHA granule-associated protein PhaP;
DE   AltName: Full=Major haloarchaeal phasin;
GN   Name=phaP; OrderedLocusNames=HFX_5219; ORFNames=BM92_17940, C439_00150;
OS   Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC
OS   14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei).
OG   Plasmid HMPLAS2, and Plasmid pHM300.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC   Haloferacaceae; Haloferax.
OX   NCBI_TaxID=523841;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX   PubMed=18408025; DOI=10.1128/jb.00134-08;
RA   Lu Q., Han J., Zhou L., Zhou J., Xiang H.;
RT   "Genetic and biochemical characterization of the poly(3-hydroxybutyrate-co-
RT   3-hydroxyvalerate) synthase in Haloferax mediterranei.";
RL   J. Bacteriol. 190:4173-4180(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX   PubMed=22247127; DOI=10.1128/aem.07114-11;
RA   Cai S., Cai L., Liu H., Liu X., Han J., Zhou J., Xiang H.;
RT   "Identification of the haloarchaeal phasin (PhaP) that functions in
RT   polyhydroxyalkanoate accumulation and granule formation in Haloferax
RT   mediterranei.";
RL   Appl. Environ. Microbiol. 78:1946-1952(2012).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RC   PLASMID=pHM300;
RX   PubMed=22843593; DOI=10.1128/jb.00880-12;
RA   Han J., Zhang F., Hou J., Liu X., Li M., Liu H., Cai L., Zhang B., Chen Y.,
RA   Zhou J., Hu S., Xiang H.;
RT   "Complete genome sequence of the metabolically versatile halophilic
RT   archaeon Haloferax mediterranei, a poly(3-hydroxybutyrate-co-3-
RT   hydroxyvalerate) producer.";
RL   J. Bacteriol. 194:4463-4464(2012).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX   PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA   Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA   Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT   "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT   strategies for static and dynamic osmo-response.";
RL   PLoS Genet. 10:E1004784-E1004784(2014).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RC   PLASMID=HMPLAS2;
RA   Bautista V.;
RT   "Transcriptional profiles of Haloferax mediterranei on the basis of
RT   nitrogen availability.";
RL   Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Polyhydroxyalkanoate (PHA) granule structural protein.
CC       Important for PHA granule formation and separation, and for cell
CC       growth. {ECO:0000269|PubMed:22247127}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic granule
CC       {ECO:0000269|PubMed:22247127}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant is defective in both PHA
CC       biosynthesis and cell growth. The number of PHA granules is
CC       significantly lower. {ECO:0000269|PubMed:22247127}.
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DR   EMBL; EU374220; ACB10368.2; -; Genomic_DNA.
DR   EMBL; JN980969; AEU08668.1; -; Genomic_DNA.
DR   EMBL; CP001870; AFK21052.1; -; Genomic_DNA.
DR   EMBL; AOLO01000001; EMA05163.1; -; Genomic_DNA.
DR   EMBL; CP007553; AHZ24088.1; -; Genomic_DNA.
DR   RefSeq; WP_004056140.1; NZ_CP039141.1.
DR   AlphaFoldDB; I3R9Z2; -.
DR   EnsemblBacteria; AFK21052; AFK21052; HFX_5219.
DR   EnsemblBacteria; AHZ24088; AHZ24088; BM92_17940.
DR   EnsemblBacteria; EMA05163; EMA05163; C439_00150.
DR   GeneID; 40158348; -.
DR   KEGG; hme:HFX_5219; -.
DR   PATRIC; fig|523841.21.peg.28; -.
DR   HOGENOM; CLU_111886_0_0_2; -.
DR   OMA; TTAFEMQ; -.
DR   OrthoDB; 98579at2157; -.
DR   Proteomes; UP000006469; Plasmid pHM300.
DR   Proteomes; UP000011603; Unassembled WGS sequence.
DR   Proteomes; UP000027075; Plasmid HMPLAS2.
PE   4: Predicted;
KW   Plasmid.
FT   CHAIN           1..154
FT                   /note="PHA granule-associated protein PhaP"
FT                   /id="PRO_0000430463"
SQ   SEQUENCE   154 AA;  17864 MW;  C251D3383AECBD3E CRC64;
     MSEQANPFTQ FFQLQRRSIE QSQRSMHQGI EFQKQVTRMM VDGMKAQQSV SRKGTDFART
     AVKSYFEMMD SSVPGDNRMY ADVKDAMDEQ FDSIDEMTEQ TWDVMEESFE ENADAMDEIM
     EQSLEYLDDA TEVYLEGLQE IEETSAQVTP DTPE