AST1_CAEEL
ID AST1_CAEEL Reviewed; 377 AA.
AC Q22355;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1999, sequence version 2.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Transcription factor ast-1 {ECO:0000305};
DE AltName: Full=axon steering defect-1 {ECO:0000312|WormBase:T08H4.3};
GN Name=ast-1 {ECO:0000312|WormBase:T08H4.3};
GN ORFNames=T08H4.3 {ECO:0000312|WormBase:T08H4.3};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP GLY-240 AND ALA-251.
RX PubMed=16584723; DOI=10.1016/j.ydbio.2006.02.042;
RA Schmid C., Schwarz V., Hutter H.;
RT "AST-1, a novel ETS-box transcription factor, controls axon guidance and
RT pharynx development in C. elegans.";
RL Dev. Biol. 293:403-413(2006).
RN [3] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF MET-263.
RX PubMed=19287374; DOI=10.1038/nature07929;
RA Flames N., Hobert O.;
RT "Gene regulatory logic of dopamine neuron differentiation.";
RL Nature 458:885-889(2009).
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE,
RP AND MUTAGENESIS OF GLY-240.
RX PubMed=22069471; DOI=10.1371/journal.pone.0026811;
RA Siehr M.S., Koo P.K., Sherlekar A.L., Bian X., Bunkers M.R., Miller R.M.,
RA Portman D.S., Lints R.;
RT "Multiple doublesex-related genes specify critical cell fates in a C.
RT elegans male neural circuit.";
RL PLoS ONE 6:E26811-E26811(2011).
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=23788625; DOI=10.1101/gad.217224.113;
RA Doitsidou M., Flames N., Topalidou I., Abe N., Felton T., Remesal L.,
RA Popovitchenko T., Mann R., Chalfie M., Hobert O.;
RT "A combinatorial regulatory signature controls terminal differentiation of
RT the dopaminergic nervous system in C. elegans.";
RL Genes Dev. 27:1391-1405(2013).
CC -!- FUNCTION: Transcription factor (PubMed:16584723, PubMed:19287374,
CC PubMed:23788625). Probably binds to DNA sequences containing the
CC consensus motif 5'-CGGA[AT][AG]-3' (PubMed:19287374). Positively
CC modulates expression of dopamine pathway genes, acting as a terminal
CC selector for differentiation of dopaminergic neurons; may act in
CC concert with homeobox proteins ceh-40, ceh-43 and ceh-20
CC (PubMed:19287374, PubMed:23788625, PubMed:22069471). Required for axon
CC navigation in some interneurons, perhaps acting in the same pathways as
CC basement membrane protein nid-1 and unc-6/netrin (PubMed:16584723).
CC Plays a role in the differentiation of the ventral cord pioneer neuron
CC AVG (PubMed:16584723). Required for morphogenesis of the pharynx
CC (PubMed:16584723). {ECO:0000269|PubMed:16584723,
CC ECO:0000269|PubMed:19287374, ECO:0000269|PubMed:22069471,
CC ECO:0000269|PubMed:23788625}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|RuleBase:RU004019,
CC ECO:0000269|PubMed:16584723, ECO:0000269|PubMed:22069471}. Cell
CC projection, neuron projection {ECO:0000269|PubMed:16584723}.
CC Note=Initially localized only in the nucleus, but later in development,
CC at about the 3-fold stage, expressed elsewhere in the cell body and
CC also in the neuronal projections. {ECO:0000269|PubMed:16584723}.
CC -!- TISSUE SPECIFICITY: Expressed in the A-neurons in the male-specific
CC genital sensilla (simple sense organs) known as rays.
CC {ECO:0000269|PubMed:22069471}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the embryo in sensory neurons with 6-
CC fold symmetry, probably the inner labial neurons (PubMed:16584723).
CC Expressed between comma and 3-fold stage, diminishing afterwards and
CC absent in larval stages and adults (PubMed:16584723). Expressed in all
CC dopaminergic neurons throughout postembryonic stages (PubMed:19287374).
CC Expressed in a few cells in the pharynx at the 2-fold stage
CC (PubMed:16584723). {ECO:0000269|PubMed:16584723,
CC ECO:0000269|PubMed:19287374}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown abolishes expression of
CC tyrosine monooxygenase cat-2 in neurons in the male-specific genital
CC sensilla (simple sense organs) known as rays.
CC {ECO:0000269|PubMed:22069471}.
CC -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
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DR EMBL; BX284602; CCD73307.1; -; Genomic_DNA.
DR PIR; T34343; T34343.
DR RefSeq; NP_001022326.1; NM_001027155.4.
DR AlphaFoldDB; Q22355; -.
DR SMR; Q22355; -.
DR STRING; 6239.T08H4.3; -.
DR EPD; Q22355; -.
DR PaxDb; Q22355; -.
DR PeptideAtlas; Q22355; -.
DR EnsemblMetazoa; T08H4.3.1; T08H4.3.1; WBGene00020368.
DR GeneID; 173749; -.
DR KEGG; cel:CELE_T08H4.3; -.
DR UCSC; T08H4.3; c. elegans.
DR CTD; 173749; -.
DR WormBase; T08H4.3; CE19576; WBGene00020368; ast-1.
DR eggNOG; KOG3806; Eukaryota.
DR HOGENOM; CLU_045216_1_0_1; -.
DR InParanoid; Q22355; -.
DR OMA; NSHAMGR; -.
DR OrthoDB; 1042747at2759; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00020368; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0043005; C:neuron projection; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR GO; GO:0007411; P:axon guidance; IMP:UniProtKB.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0071542; P:dopaminergic neuron differentiation; IMP:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; IMP:UniProtKB.
DR GO; GO:0048664; P:neuron fate determination; IMP:UniProtKB.
DR GO; GO:0110040; P:pharynx morphogenesis; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0110037; P:regulation of nematode male tail tip morphogenesis; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000418; Ets_dom.
DR InterPro; IPR046328; ETS_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR11849; PTHR11849; 3.
DR Pfam; PF00178; Ets; 1.
DR PRINTS; PR00454; ETSDOMAIN.
DR SMART; SM00413; ETS; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR PROSITE; PS50061; ETS_DOMAIN_3; 1.
PE 1: Evidence at protein level;
KW Cell projection; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..377
FT /note="Transcription factor ast-1"
FT /id="PRO_0000454715"
FT DNA_BIND 214..294
FT /note="ETS"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00237"
FT REGION 72..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 240
FT /note="G->R: In rh300; interneuron axon guidance defects in
FT the ventral nerve cord (VNC). Partial differentiation
FT defects of the VNC pioneer neuron, AVG. Abolishes
FT expression of tyrosine monooxygenase cat-2 in the male-
FT specific ray neurons and other dopaminergic neurons."
FT /evidence="ECO:0000269|PubMed:16584723,
FT ECO:0000269|PubMed:19287374, ECO:0000269|PubMed:22069471"
FT MUTAGEN 251
FT /note="A->V: In hd1; interneuron axon guidance defects in
FT the ventral nerve cord (VNC). Reduced expression of
FT tyrosine monooxygenase cat-2 in dopaminergic neurons."
FT /evidence="ECO:0000269|PubMed:16584723,
FT ECO:0000269|PubMed:19287374"
FT MUTAGEN 263
FT /note="M->I: In ot417; defects in dopaminergic neuron fate.
FT Reduced expression of tyrosine monooxygenase cat-2 in
FT dopaminergic neurons."
FT /evidence="ECO:0000269|PubMed:19287374"
SQ SEQUENCE 377 AA; 41466 MW; D78A6BFA2472D409 CRC64;
MQVVSSATLP PTVASADLAI VGGRSSDEDV IKYSAIQTIK QEQQQQQQQQ SNTALPSYNF
PFFNGMQNDF PPNRMLYNDN TMQKSENDHF TGMNLSTSAS SSGNSTSSKD QSRRQFYTES
SNSSGNGAAA TSGSNGSSSS TESKSDVFNI SMNAFAATPG SKSEDHNIPS FNMLSSYYTG
ALKLSNSTSF ANPDPYQILG PTSKNLAHSG SGQTQLWQFL LELLSDKRYS EVITWEGTQG
EFKLVDPDEV ARKWGERKSK PNMNYDKMSR ALRYYYDKNI MAKVHGKRYA YKFDFQGIAQ
ALQPPTASHP QDYFNSHAMG RIAPDFSSWT SANYRSLNIA GFNNGSTIFN PSVNYSAFGA
TGTSNNLSAA RAFPLYR
