ID   AST2_YEAST              Reviewed;         430 AA.
AC   P39945; D3DM08;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 148.
DE   RecName: Full=Protein AST2 {ECO:0000305};
DE   AltName: Full=ATPAse stabilizing protein 1 {ECO:0000303|PubMed:7822420};
GN   Name=AST2 {ECO:0000303|PubMed:7822420}; OrderedLocusNames=YER101C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169868;
RA   Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA   Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA   Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA   Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA   Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA   Botstein D., Davis R.W.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL   Nature 387:78-81(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   FUNCTION.
RX   PubMed=7822420; DOI=10.1083/jcb.128.1.39;
RA   Chang A., Fink G.R.;
RT   "Targeting of the yeast plasma membrane [H+]ATPase: a novel gene AST1
RT   prevents mislocalization of mutant ATPase to the vacuole.";
RL   J. Cell Biol. 128:39-49(1995).
RN   [4]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Lipid raft-associated protein involved in the targeting of
CC       PMA1 from Golgi to the plasma membrane (Probable). May induce
CC       clustering of PMA1, which facilitates partition of PMA1 into lipid
CC       rafts after leaving the ER its and transport to the cell surface (By
CC       similarity). {ECO:0000250|UniProtKB:P35183,
CC       ECO:0000305|PubMed:7822420}.
CC   -!- MISCELLANEOUS: Present with 937 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; U18839; AAB64656.1; -; Genomic_DNA.
DR   EMBL; BK006939; DAA07762.1; -; Genomic_DNA.
DR   PIR; S50604; S50604.
DR   RefSeq; NP_011027.1; NM_001178992.1.
DR   AlphaFoldDB; P39945; -.
DR   SMR; P39945; -.
DR   BioGRID; 36847; 125.
DR   DIP; DIP-2914N; -.
DR   IntAct; P39945; 4.
DR   MINT; P39945; -.
DR   STRING; 4932.YER101C; -.
DR   iPTMnet; P39945; -.
DR   MaxQB; P39945; -.
DR   PaxDb; P39945; -.
DR   PRIDE; P39945; -.
DR   EnsemblFungi; YER101C_mRNA; YER101C; YER101C.
DR   GeneID; 856838; -.
DR   KEGG; sce:YER101C; -.
DR   SGD; S000000903; AST2.
DR   VEuPathDB; FungiDB:YER101C; -.
DR   eggNOG; KOG1198; Eukaryota.
DR   GeneTree; ENSGT00940000176396; -.
DR   HOGENOM; CLU_026673_4_0_1; -.
DR   InParanoid; P39945; -.
DR   OMA; IGREYSG; -.
DR   BioCyc; YEAST:G3O-30266-MON; -.
DR   PRO; PR:P39945; -.
DR   Proteomes; UP000002311; Chromosome V.
DR   RNAct; P39945; protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   GO; GO:0006612; P:protein targeting to membrane; IGI:SGD.
DR   InterPro; IPR013154; ADH_N.
DR   InterPro; IPR011032; GroES-like_sf.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020843; PKS_ER.
DR   Pfam; PF08240; ADH_N; 1.
DR   SMART; SM00829; PKS_ER; 1.
DR   SUPFAM; SSF50129; SSF50129; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..430
FT                   /note="Protein AST2"
FT                   /id="PRO_0000064711"
SQ   SEQUENCE   430 AA;  48371 MW;  FBEC2F2CDE894F84 CRC64;
     MAEKILENKD PKLEAMTVDH EVSAPKPIPV DEPTLTRVAR PLRHVRHIPV KSLVFHSKHG
     PITFSYENKI KLPISKNKLV VQVNYVGLNP VDMKIRNGYT KPIYGEAGIG REYSGVITHV
     GDNLTNRWNV GDDVYGIYYH PKLAIGALQS SLLIDPRVDP ILMRPKNTLS PEKAAGSLFC
     LGTALNLLAQ LKEKDQLNTE SNVLINGGTS SVGMFAIQLL KRYYKVSKKL VVVTSGNGAA
     VLSEHFPDLK DEIIFINYLS CRGKSSKPLR RMLDTGKVVD YDDFNTLKET EDYTQGKFNV
     VLDFIGGYDI LSHSSSLIHA KGAYITTVGD YVGNYKKDVF DSWDNPSANA RKMFGSMLWS
     YDYSHFYFDP NIKIIPKKND WIHECGKLLN EGVVDCVVDK VYSWKNFKEA FSYMATQRAQ
     GKLIMKVEGF