ID   PHAZ_PSEFL              Reviewed;         278 AA.
AC   Q51718;
DT   20-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 64.
DE   RecName: Full=Poly(3-hydroxyoctanoate) depolymerase;
DE            Short=PHA depolymerase;
DE            Short=PHO depolymerase;
DE            Short=[P(3HO)] depolymerase;
DE            EC=3.1.1.76;
DE   Flags: Precursor;
GN   Name=phaZ;
OS   Pseudomonas fluorescens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=294;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, AND
RP   CHARACTERIZATION.
RC   STRAIN=DSM 7139 / GK13;
RX   PubMed=7961472; DOI=10.1128/jb.176.22.7065-7073.1994;
RA   Schirmer A., Jendrossek D.;
RT   "Molecular characterization of the extracellular poly(3-hydroxyoctanoic
RT   acid) [P(3HO)] depolymerase gene of Pseudomonas fluorescens GK13 and of its
RT   gene product.";
RL   J. Bacteriol. 176:7065-7073(1994).
CC   -!- FUNCTION: Hydrolysis of poly(3-hydroxyoctanoic acid).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes the polyester poly{oxycarbonyl[(R)-2-
CC         pentylethylene]} to oligomers.; EC=3.1.1.76;
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U10470; AAA64538.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q51718; -.
DR   SMR; Q51718; -.
DR   KEGG; ag:AAA64538; -.
DR   BioCyc; MetaCyc:MON-15975; -.
DR   BRENDA; 3.1.1.75; 5121.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050527; F:poly(3-hydroxyoctanoate) depolymerase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Secreted; Signal.
FT   SIGNAL          1..33
FT   CHAIN           34..278
FT                   /note="Poly(3-hydroxyoctanoate) depolymerase"
FT                   /id="PRO_0000022046"
SQ   SEQUENCE   278 AA;  30267 MW;  76744C0B24F170A0 CRC64;
     MPLRTLLCGL LLAVCLGQHA LAASRCSERP RTLLRPAEVS CSYQSTWLDS GLVGQRKIIY
     QTPLGTPPAG GWPVVLIYQG SFFPLNDFSY HSNLPFGGYY EGKLVQNLLD HGYAVIAPSA
     PADLFWQTNI PGLAQAYELS TDYDFLGNVL AAIASGHFGP LNAQRQYATG ISSGGYNTSR
     MAVSFPGKFR ALAVQSGSYA TCSGPLCVVP DQLPADHPPT LFLHGFVDAV VPWWSMDLYY
     DRLLHQGIET ARYTEPLGGH EWFAASPGKV LAWFNAHP