PHB1_ARATH
ID PHB1_ARATH Reviewed; 288 AA.
AC O49460; Q8LA39;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Prohibitin-1, mitochondrial;
DE Short=Atphb1;
GN Name=PHB1; OrderedLocusNames=At4g28510; ORFNames=F20O9.200;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. C24, and cv. Columbia;
RA Sun L., Goodman H.M.;
RT "Arabidopsis genes encoding prohibitin: importance for early development.";
RL Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, AND SUBUNIT.
RX PubMed=12837548; DOI=10.1016/s0005-2728(03)00045-8;
RA Heazlewood J.L., Howell K.A., Millar A.H.;
RT "Mitochondrial complex I from Arabidopsis and rice: orthologs of mammalian
RT and fungal components coupled with plant-specific subunits.";
RL Biochim. Biophys. Acta 1604:159-169(2003).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=14671022; DOI=10.1105/tpc.016055;
RA Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J.,
RA Millar A.H.;
RT "Experimental analysis of the Arabidopsis mitochondrial proteome highlights
RT signaling and regulatory components, provides assessment of targeting
RT prediction programs, and indicates plant-specific mitochondrial proteins.";
RL Plant Cell 16:241-256(2004).
RN [8]
RP TISSUE SPECIFICITY, SUBUNIT, SUBCELLULAR LOCATION, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC STRAIN=cv. Columbia;
RX PubMed=17883375; DOI=10.1111/j.1365-313x.2007.03276.x;
RA Van Aken O., Pecenkova T., van de Cotte B., De Rycke R., Eeckhout D.,
RA Fromm H., De Jaeger G., Witters E., Beemster G.T.S., Inze D.,
RA Van Breusegem F.;
RT "Mitochondrial type-I prohibitins of Arabidopsis thaliana are required for
RT supporting proficient meristem development.";
RL Plant J. 52:850-864(2007).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=18189341; DOI=10.1021/pr700595p;
RA Meyer E.H., Taylor N.L., Millar A.H.;
RT "Resolving and identifying protein components of plant mitochondrial
RT respiratory complexes using three dimensions of gel electrophoresis.";
RL J. Proteome Res. 7:786-794(2008).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=21841088; DOI=10.1104/pp.111.182352;
RA Klodmann J., Senkler M., Rode C., Braun H.-P.;
RT "Defining the protein complex proteome of plant mitochondria.";
RL Plant Physiol. 157:587-598(2011).
CC -!- FUNCTION: Prohibitin probably acts as a holdase/unfoldase for the
CC stabilization of newly synthesized mitochondrial proteins.
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of a prohibitin multimeric complex in mitochondrial
CC membranes. {ECO:0000269|PubMed:12837548, ECO:0000269|PubMed:17883375,
CC ECO:0000269|PubMed:18189341}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:12837548, ECO:0000269|PubMed:14671022,
CC ECO:0000269|PubMed:17883375, ECO:0000269|PubMed:18189341,
CC ECO:0000269|PubMed:21841088}; Single-pass type II membrane protein
CC {ECO:0000269|PubMed:12837548, ECO:0000269|PubMed:14671022,
CC ECO:0000269|PubMed:17883375, ECO:0000269|PubMed:18189341,
CC ECO:0000269|PubMed:21841088}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in proliferative tissues,
CC including vasculature, shoot and root apical tissues.
CC {ECO:0000269|PubMed:17883375}.
CC -!- SIMILARITY: Belongs to the prohibitin family. {ECO:0000305}.
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DR EMBL; U66591; AAD00155.1; -; mRNA.
DR EMBL; U66594; AAD00158.1; -; Genomic_DNA.
DR EMBL; AL021749; CAA16891.1; -; Genomic_DNA.
DR EMBL; AL161573; CAB81439.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85496.1; -; Genomic_DNA.
DR EMBL; AY093228; AAM13227.1; -; mRNA.
DR EMBL; BT000211; AAN15530.1; -; mRNA.
DR EMBL; AY088047; AAM65593.1; -; mRNA.
DR PIR; T04622; T04622.
DR RefSeq; NP_194580.1; NM_118993.4.
DR AlphaFoldDB; O49460; -.
DR SMR; O49460; -.
DR BioGRID; 14256; 6.
DR IntAct; O49460; 4.
DR STRING; 3702.AT4G28510.1; -.
DR PaxDb; O49460; -.
DR PRIDE; O49460; -.
DR ProMEX; O49460; -.
DR ProteomicsDB; 236726; -.
DR EnsemblPlants; AT4G28510.1; AT4G28510.1; AT4G28510.
DR GeneID; 828969; -.
DR Gramene; AT4G28510.1; AT4G28510.1; AT4G28510.
DR KEGG; ath:AT4G28510; -.
DR Araport; AT4G28510; -.
DR TAIR; locus:2121392; AT4G28510.
DR eggNOG; KOG3090; Eukaryota.
DR HOGENOM; CLU_047969_0_2_1; -.
DR InParanoid; O49460; -.
DR OMA; IKYTRLG; -.
DR OrthoDB; 1089994at2759; -.
DR PhylomeDB; O49460; -.
DR PRO; PR:O49460; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O49460; baseline and differential.
DR Genevisible; O49460; AT.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; HDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0007005; P:mitochondrion organization; IBA:GO_Central.
DR CDD; cd03401; SPFH_prohibitin; 1.
DR Gene3D; 3.30.479.30; -; 1.
DR InterPro; IPR001107; Band_7.
DR InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR InterPro; IPR000163; Prohibitin.
DR PANTHER; PTHR23222; PTHR23222; 1.
DR Pfam; PF01145; Band_7; 1.
DR PRINTS; PR00679; PROHIBITIN.
DR SMART; SM00244; PHB; 1.
DR SUPFAM; SSF117892; SSF117892; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..288
FT /note="Prohibitin-1, mitochondrial"
FT /id="PRO_0000420596"
FT TOPO_DOM 1..10
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..30
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..288
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT COILED 186..219
FT /evidence="ECO:0000255"
SQ SEQUENCE 288 AA; 31706 MW; A08BE4720B6BF1A8 CRC64;
MNNVKVPKIP GGGAISTLLK VGIIGGLGLY GATHSLYNVE GGHRAIMFNR LVGIKDKVYP
EGTHLMIPWF ERPVIYDVRA RPYLVESTSG SRDLQMVKIG LRVLTRPMAD QLPEIYRSLG
ENYSERVLPS IINETLKAVV AQYNASQLIT QREAVSREIR KILTERAANF NVALDDVSIT
NLTFGKEFTA AIEAKQVAAQ EAERAKFIVE KAEQDKRSAV IRAQGEAKSA QLIGQAIANN
QAFITLRKIE AAREIAQTIA NSANKVYLSS DDLLLNLQGM NLDVDAKN
