AST3_DROME
ID AST3_DROME Reviewed; 257 AA.
AC P09774; Q24387; Q8MRA5;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2001, sequence version 2.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=Achaete-scute complex protein T3;
DE AltName: Full=Protein lethal of scute;
DE Short=Lethal of sc;
GN Name=l(1)sc; Synonyms=l'sc, T3; ORFNames=CG3839;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=Canton-S;
RX PubMed=2461300; DOI=10.1002/j.1460-2075.1988.tb03108.x;
RA Alonso M.C., Cabrera C.V.;
RT "The achaete-scute gene complex of Drosophila melanogaster comprises four
RT homologous genes.";
RL EMBO J. 7:2585-2591(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Canton-S; TISSUE=Embryo;
RX PubMed=8076513; DOI=10.1242/dev.118.3.1003;
RA Martin-Bermudo M.D., Gonzalez F., Dominguez M., Rodriguez I.,
RA Ruiz-Gomez M., Romani S., Modolell J., Jimenez F.;
RT "Molecular characterization of the lethal of scute genetic function.";
RL Development 118:1003-1012(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: AS-C proteins are involved in the determination of the
CC neuronal precursors in the peripheral nervous system and the central
CC nervous system.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein.
CC -!- TISSUE SPECIFICITY: L(1)SC, SC and AC strongly label the presumptive
CC stomatogastric nervous system, while ASE is more prominent in the
CC presumptive procephalic lobe.
CC -!- MISCELLANEOUS: AS-C proteins are expressed first earlier in ectoderm
CC and then in the internalized neuroblasts, while T8 is expressed in
CC presumptive neural precursors, once they have segregated from the
CC ectoderm.
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DR EMBL; X12549; CAA31065.1; -; Genomic_DNA.
DR EMBL; X71806; CAA50680.1; -; mRNA.
DR EMBL; AE014298; AAF45500.1; -; Genomic_DNA.
DR EMBL; AL024453; CAA19656.1; -; Genomic_DNA.
DR EMBL; AY121693; AAM52020.1; -; mRNA.
DR PIR; S01165; S01165.
DR RefSeq; NP_476623.1; NM_057275.5.
DR AlphaFoldDB; P09774; -.
DR SMR; P09774; -.
DR BioGRID; 57556; 17.
DR IntAct; P09774; 12.
DR STRING; 7227.FBpp0070073; -.
DR PaxDb; P09774; -.
DR DNASU; 30983; -.
DR EnsemblMetazoa; FBtr0070074; FBpp0070073; FBgn0002561.
DR GeneID; 30983; -.
DR KEGG; dme:Dmel_CG3839; -.
DR FlyBase; FBgn0002561; l(1)sc.
DR VEuPathDB; VectorBase:FBgn0002561; -.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00940000170891; -.
DR HOGENOM; CLU_063523_0_0_1; -.
DR InParanoid; P09774; -.
DR OMA; PYGEQLP; -.
DR OrthoDB; 1131543at2759; -.
DR PhylomeDB; P09774; -.
DR SignaLink; P09774; -.
DR BioGRID-ORCS; 30983; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 30983; -.
DR PRO; PR:P09774; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0002561; Expressed in ectoderm anlage and 54 other tissues.
DR Genevisible; P09774; DM.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central.
DR GO; GO:0005667; C:transcription regulator complex; IPI:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:FlyBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0007417; P:central nervous system development; NAS:FlyBase.
DR GO; GO:0061331; P:epithelial cell proliferation involved in Malpighian tubule morphogenesis; IMP:FlyBase.
DR GO; GO:0061382; P:Malpighian tubule tip cell differentiation; IMP:FlyBase.
DR GO; GO:0007400; P:neuroblast fate determination; IMP:FlyBase.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0007422; P:peripheral nervous system development; NAS:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:FlyBase.
DR GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0007423; P:sensory organ development; IBA:GO_Central.
DR GO; GO:0007419; P:ventral cord development; TAS:FlyBase.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR015660; MASH1/Ascl1a-like.
DR PANTHER; PTHR13935; PTHR13935; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Neurogenesis;
KW Reference proteome.
FT CHAIN 1..257
FT /note="Achaete-scute complex protein T3"
FT /id="PRO_0000127135"
FT DOMAIN 83..145
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 161..221
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 161..215
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 198
FT /note="H -> R (in Ref. 1; CAA31065)"
FT /evidence="ECO:0000305"
FT CONFLICT 257
FT /note="Q -> R (in Ref. 6; AAM52020)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 257 AA; 28972 MW; 1AEC8FA0B3144F87 CRC64;
MTSICSSKFQ QQHYQLTNSN IFLLQHQHHH QTQQHQLIAP KIPLGTSQLQ NMQQSQQSNV
GPMLSSQKKK FNYNNMPYGE QLPSVARRNA RERNRVKQVN NGFVNLRQHL PQTVVNSLSN
GGRGSSKKLS KVDTLRIAVE YIRGLQDMLD DGTASSTRHI YNSADESSND GSSYNDYNDS
LDSSQQFLTG ATQSAQSHSY HSASPTPSYS GSEISGGGYI KQELQEQDLK FDSFDSFSDE
QPDDEELLDY ISSWQEQ
