PHB3_ARATH
ID PHB3_ARATH Reviewed; 277 AA.
AC O04331;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 25-MAY-2022, entry version 131.
DE RecName: Full=Prohibitin-3, mitochondrial;
DE Short=Atphb3;
DE AltName: Full=Protein ENHANCED ETHYLENE RESPONSE 3;
GN Name=PHB3; Synonyms=EER3; OrderedLocusNames=At5g40770; ORFNames=K1B16.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=9132067; DOI=10.1023/a:1005737026289;
RA Snedden W.A., Fromm H.;
RT "Characterization of the plant homologue of prohibitin, a gene associated
RT with antiproliferative activity in mammalian cells.";
RL Plant Mol. Biol. 33:753-756(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Sun L., Goodman H.M.;
RT "Arabidopsis genes encoding prohibitin: importance for early development.";
RL Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, AND SUBUNIT.
RX PubMed=12837548; DOI=10.1016/s0005-2728(03)00045-8;
RA Heazlewood J.L., Howell K.A., Millar A.H.;
RT "Mitochondrial complex I from Arabidopsis and rice: orthologs of mammalian
RT and fungal components coupled with plant-specific subunits.";
RL Biochim. Biophys. Acta 1604:159-169(2003).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=15060130; DOI=10.1074/mcp.m400001-mcp200;
RA Marmagne A., Rouet M.-A., Ferro M., Rolland N., Alcon C., Joyard J.,
RA Garin J., Barbier-Brygoo H., Ephritikhine G.;
RT "Identification of new intrinsic proteins in Arabidopsis plasma membrane
RT proteome.";
RL Mol. Cell. Proteomics 3:675-691(2004).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=14671022; DOI=10.1105/tpc.016055;
RA Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J.,
RA Millar A.H.;
RT "Experimental analysis of the Arabidopsis mitochondrial proteome highlights
RT signaling and regulatory components, provides assessment of targeting
RT prediction programs, and indicates plant-specific mitochondrial proteins.";
RL Plant Cell 16:241-256(2004).
RN [10]
RP FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF ASP-165, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=17525078; DOI=10.1093/jxb/erm086;
RA Christians M.J., Larsen P.B.;
RT "Mutational loss of the prohibitin AtPHB3 results in an extreme
RT constitutive ethylene response phenotype coupled with partial loss of
RT ethylene-inducible gene expression in Arabidopsis seedlings.";
RL J. Exp. Bot. 58:2237-2248(2007).
RN [11]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBUNIT, SUBCELLULAR
RP LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=cv. Columbia;
RX PubMed=17883375; DOI=10.1111/j.1365-313x.2007.03276.x;
RA Van Aken O., Pecenkova T., van de Cotte B., De Rycke R., Eeckhout D.,
RA Fromm H., De Jaeger G., Witters E., Beemster G.T.S., Inze D.,
RA Van Breusegem F.;
RT "Mitochondrial type-I prohibitins of Arabidopsis thaliana are required for
RT supporting proficient meristem development.";
RL Plant J. 52:850-864(2007).
RN [12]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=18189341; DOI=10.1021/pr700595p;
RA Meyer E.H., Taylor N.L., Millar A.H.;
RT "Resolving and identifying protein components of plant mitochondrial
RT respiratory complexes using three dimensions of gel electrophoresis.";
RL J. Proteome Res. 7:786-794(2008).
RN [13]
RP FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF GLY-37.
RX PubMed=20068191; DOI=10.1105/tpc.109.072066;
RA Wang Y., Ries A., Wu K., Yang A., Crawford N.M.;
RT "The Arabidopsis prohibitin gene PHB3 functions in nitric oxide-mediated
RT responses and in hydrogen peroxide-induced nitric oxide accumulation.";
RL Plant Cell 22:249-259(2010).
RN [14]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=21841088; DOI=10.1104/pp.111.182352;
RA Klodmann J., Senkler M., Rode C., Braun H.-P.;
RT "Defining the protein complex proteome of plant mitochondria.";
RL Plant Physiol. 157:587-598(2011).
RN [15]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT GLY-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
CC -!- FUNCTION: Prohibitin probably acts as a holdase/unfoldase for the
CC stabilization of newly synthesized mitochondrial proteins (By
CC similarity). Necessary for mitochondrial and cell metabolism and
CC biogenesis. Required to regulate the ethylene-mediated signaling;
CC involved in growth maintenance in the presence of ethylene. Functions
CC in nitric oxide (NO)-mediated responses and in hydrogen peroxide-
CC induced NO accumulation. {ECO:0000250, ECO:0000269|PubMed:17525078,
CC ECO:0000269|PubMed:17883375, ECO:0000269|PubMed:20068191}.
CC -!- SUBUNIT: Component of a prohibitin multimeric complex in mitochondrial
CC membranes. {ECO:0000269|PubMed:12837548, ECO:0000269|PubMed:17883375,
CC ECO:0000269|PubMed:18189341}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15060130}.
CC Mitochondrion inner membrane {ECO:0000269|PubMed:12837548,
CC ECO:0000269|PubMed:14671022, ECO:0000269|PubMed:17883375,
CC ECO:0000269|PubMed:18189341, ECO:0000269|PubMed:21841088,
CC ECO:0000269|PubMed:9132067}; Single-pass type II membrane protein
CC {ECO:0000255}. Nucleus {ECO:0000269|PubMed:17525078}. Cytoplasm
CC {ECO:0000269|PubMed:17525078}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in proliferative tissues,
CC including vasculature, shoot and root apical tissues. Expressed in
CC roots, stems, leaves and flowers (at protein level).
CC -!- DISRUPTION PHENOTYPE: Extreme constitutive ethylene response in air
CC associated with a partial loss of ethylene-inducible gene expression
CC and an increased ethylene production. Mitochondrial swelling, decreased
CC meristematic cell production, increased cell division time and reduced
CC cell expansion rates, leading to severe growth retardation. Reduced
CC sensitivity to salt stress and defective in H(2)O(2)-induced nitric
CC oxide (NO) accumulation, light-induced NO in cotyledons, abscisic acid
CC (ABA)-induced NO accumulation and stomatal closure, and in auxin-
CC induced lateral root formation. {ECO:0000269|PubMed:17525078,
CC ECO:0000269|PubMed:17883375, ECO:0000269|PubMed:20068191}.
CC -!- SIMILARITY: Belongs to the prohibitin family. {ECO:0000305}.
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DR EMBL; U69155; AAC49691.1; -; mRNA.
DR EMBL; U66593; AAD00157.1; -; mRNA.
DR EMBL; AB015470; BAB08838.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94592.1; -; Genomic_DNA.
DR EMBL; AY054499; AAK96690.1; -; mRNA.
DR EMBL; AY114631; AAM47950.1; -; mRNA.
DR EMBL; AY087641; AAM65180.1; -; mRNA.
DR RefSeq; NP_198893.1; NM_123442.5.
DR AlphaFoldDB; O04331; -.
DR SMR; O04331; -.
DR BioGRID; 19328; 29.
DR IntAct; O04331; 3.
DR STRING; 3702.AT5G40770.1; -.
DR iPTMnet; O04331; -.
DR PaxDb; O04331; -.
DR PRIDE; O04331; -.
DR ProMEX; O04331; -.
DR ProteomicsDB; 235078; -.
DR EnsemblPlants; AT5G40770.1; AT5G40770.1; AT5G40770.
DR GeneID; 834077; -.
DR Gramene; AT5G40770.1; AT5G40770.1; AT5G40770.
DR KEGG; ath:AT5G40770; -.
DR Araport; AT5G40770; -.
DR TAIR; locus:2154810; AT5G40770.
DR eggNOG; KOG3083; Eukaryota.
DR HOGENOM; CLU_047969_0_2_1; -.
DR InParanoid; O04331; -.
DR OMA; SAIEHKQ; -.
DR OrthoDB; 1089994at2759; -.
DR PhylomeDB; O04331; -.
DR PRO; PR:O04331; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O04331; baseline and differential.
DR Genevisible; O04331; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; HDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0044877; F:protein-containing complex binding; IPI:TAIR.
DR GO; GO:0051301; P:cell division; IMP:TAIR.
DR GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR GO; GO:0048527; P:lateral root development; IMP:TAIR.
DR GO; GO:0007005; P:mitochondrion organization; IMP:TAIR.
DR GO; GO:0051782; P:negative regulation of cell division; IMP:TAIR.
DR GO; GO:0009733; P:response to auxin; IEP:TAIR.
DR GO; GO:0009723; P:response to ethylene; IMP:UniProtKB.
DR GO; GO:0071731; P:response to nitric oxide; IMP:TAIR.
DR GO; GO:0009651; P:response to salt stress; IMP:TAIR.
DR GO; GO:0009697; P:salicylic acid biosynthetic process; IMP:TAIR.
DR CDD; cd03401; SPFH_prohibitin; 1.
DR Gene3D; 3.30.479.30; -; 1.
DR InterPro; IPR001107; Band_7.
DR InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR InterPro; IPR000163; Prohibitin.
DR PANTHER; PTHR23222; PTHR23222; 1.
DR Pfam; PF01145; Band_7; 1.
DR PRINTS; PR00679; PROHIBITIN.
DR SMART; SM00244; PHB; 1.
DR SUPFAM; SSF117892; SSF117892; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell membrane; Cytoplasm; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Nucleus; Reference proteome; Signal-anchor;
KW Transmembrane; Transmembrane helix.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..277
FT /note="Prohibitin-3, mitochondrial"
FT /id="PRO_0000420598"
FT TOPO_DOM 2..6
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..28
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 29..277
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylglycine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT MUTAGEN 37
FT /note="G->D: In phb3-3; reduced sensitivity to salt stress
FT and defective in H(2)O(2)-induced nitric oxide (NO)
FT accumulation, light-induced NO in cotyledons, abscisic acid
FT (ABA)-induced NO accumulation and stomatal closure, and in
FT auxin-induced lateral root formation."
FT /evidence="ECO:0000269|PubMed:20068191"
FT MUTAGEN 165
FT /note="D->N: In eer3-1; increased sensitivity and profound
FT exaggeration of response to ethylene, as well as increased
FT ethylene production."
FT /evidence="ECO:0000269|PubMed:17525078"
SQ SEQUENCE 277 AA; 30400 MW; 1D57190F11370693 CRC64;
MGSQQAAVSF LSNLAKAAFG LGTAATVLNT SLFTVDGGER AVIFDRFRGV MDQTVGEGTH
FLIPILQRPH IFDIRTKPHT FSSISGTKDL QMVNLTLRVL SRPEVSRLPY IFQTLGLEYD
EKVLPSIGNE VLKAVVAQFN ADQLLTERPH VSALVRESLI TRAKDFNIVL DDVAITHLSY
GVEFSRAVEQ KQVAQQEAER SKFVVMKADQ ERRAAVIRAE GESEAAQLIS DATAKAGMGL
IELRRIEASR EIASTLARSP NVAYLPGGQS MLFALNR
