PHB6_ARATH
ID PHB6_ARATH Reviewed; 286 AA.
AC Q9SIL6;
DT 09-JAN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Prohibitin-6, mitochondrial;
DE Short=Atphb6;
GN Name=PHB6; OrderedLocusNames=At2g20530; ORFNames=T13C7.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY, AND SUBUNIT.
RX PubMed=12837548; DOI=10.1016/s0005-2728(03)00045-8;
RA Heazlewood J.L., Howell K.A., Millar A.H.;
RT "Mitochondrial complex I from Arabidopsis and rice: orthologs of mammalian
RT and fungal components coupled with plant-specific subunits.";
RL Biochim. Biophys. Acta 1604:159-169(2003).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=14671022; DOI=10.1105/tpc.016055;
RA Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J.,
RA Millar A.H.;
RT "Experimental analysis of the Arabidopsis mitochondrial proteome highlights
RT signaling and regulatory components, provides assessment of targeting
RT prediction programs, and indicates plant-specific mitochondrial proteins.";
RL Plant Cell 16:241-256(2004).
RN [7]
RP TISSUE SPECIFICITY, SUBUNIT, SUBCELLULAR LOCATION, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC STRAIN=cv. Columbia;
RX PubMed=17883375; DOI=10.1111/j.1365-313x.2007.03276.x;
RA Van Aken O., Pecenkova T., van de Cotte B., De Rycke R., Eeckhout D.,
RA Fromm H., De Jaeger G., Witters E., Beemster G.T.S., Inze D.,
RA Van Breusegem F.;
RT "Mitochondrial type-I prohibitins of Arabidopsis thaliana are required for
RT supporting proficient meristem development.";
RL Plant J. 52:850-864(2007).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=18189341; DOI=10.1021/pr700595p;
RA Meyer E.H., Taylor N.L., Millar A.H.;
RT "Resolving and identifying protein components of plant mitochondrial
RT respiratory complexes using three dimensions of gel electrophoresis.";
RL J. Proteome Res. 7:786-794(2008).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=21841088; DOI=10.1104/pp.111.182352;
RA Klodmann J., Senkler M., Rode C., Braun H.-P.;
RT "Defining the protein complex proteome of plant mitochondria.";
RL Plant Physiol. 157:587-598(2011).
CC -!- FUNCTION: Prohibitin probably acts as a holdase/unfoldase for the
CC stabilization of newly synthesized mitochondrial proteins.
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of a prohibitin multimeric complex in mitochondrial
CC membranes. {ECO:0000269|PubMed:12837548, ECO:0000269|PubMed:17883375,
CC ECO:0000269|PubMed:18189341}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:12837548, ECO:0000269|PubMed:14671022,
CC ECO:0000269|PubMed:17883375, ECO:0000269|PubMed:18189341,
CC ECO:0000269|PubMed:21841088}; Single-pass type II membrane protein
CC {ECO:0000269|PubMed:12837548, ECO:0000269|PubMed:14671022,
CC ECO:0000269|PubMed:17883375, ECO:0000269|PubMed:18189341,
CC ECO:0000269|PubMed:21841088}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in proliferative tissues,
CC including vasculature, shoot and root apical tissues.
CC {ECO:0000269|PubMed:17883375}.
CC -!- SIMILARITY: Belongs to the prohibitin family. {ECO:0000305}.
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DR EMBL; AC007109; AAD25653.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07023.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07024.1; -; Genomic_DNA.
DR EMBL; AY072378; AAL62370.1; -; mRNA.
DR EMBL; AY114620; AAM47939.1; -; mRNA.
DR EMBL; AY088363; AAM65902.1; -; mRNA.
DR PIR; D84590; D84590.
DR RefSeq; NP_001077924.1; NM_001084455.1.
DR RefSeq; NP_179643.1; NM_127613.3.
DR AlphaFoldDB; Q9SIL6; -.
DR SMR; Q9SIL6; -.
DR BioGRID; 1928; 8.
DR IntAct; Q9SIL6; 2.
DR STRING; 3702.AT2G20530.2; -.
DR PaxDb; Q9SIL6; -.
DR PRIDE; Q9SIL6; -.
DR ProteomicsDB; 236746; -.
DR EnsemblPlants; AT2G20530.1; AT2G20530.1; AT2G20530.
DR EnsemblPlants; AT2G20530.2; AT2G20530.2; AT2G20530.
DR GeneID; 816575; -.
DR Gramene; AT2G20530.1; AT2G20530.1; AT2G20530.
DR Gramene; AT2G20530.2; AT2G20530.2; AT2G20530.
DR KEGG; ath:AT2G20530; -.
DR Araport; AT2G20530; -.
DR TAIR; locus:2054779; AT2G20530.
DR eggNOG; KOG3090; Eukaryota.
DR HOGENOM; CLU_047969_0_2_1; -.
DR InParanoid; Q9SIL6; -.
DR OMA; LMIPWIE; -.
DR OrthoDB; 1089994at2759; -.
DR PhylomeDB; Q9SIL6; -.
DR PRO; PR:Q9SIL6; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SIL6; baseline and differential.
DR Genevisible; Q9SIL6; AT.
DR GO; GO:0005747; C:mitochondrial respiratory chain complex I; IDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0007005; P:mitochondrion organization; IBA:GO_Central.
DR CDD; cd03401; SPFH_prohibitin; 1.
DR Gene3D; 3.30.479.30; -; 1.
DR InterPro; IPR001107; Band_7.
DR InterPro; IPR036013; Band_7/SPFH_dom_sf.
DR InterPro; IPR000163; Prohibitin.
DR PANTHER; PTHR23222; PTHR23222; 1.
DR Pfam; PF01145; Band_7; 1.
DR PRINTS; PR00679; PROHIBITIN.
DR SMART; SM00244; PHB; 1.
DR SUPFAM; SSF117892; SSF117892; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..286
FT /note="Prohibitin-6, mitochondrial"
FT /id="PRO_0000420601"
FT TOPO_DOM 1..12
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT TRANSMEM 13..35
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 36..286
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
SQ SEQUENCE 286 AA; 31637 MW; ABB380A2DA93CEE4 CRC64;
MNFKNVKVPK GPGGGVIAAV VIGGLSLYGA THTLYNVDGG HRAIVFNRLV GIKDKVYPEG
THLMIPWFER PIIYDVRAKP YLVESTSGSR DLQMVKIGLR VLTRPMADQL PEVYRSLGEN
YRERVLPSII HETLKAVVAQ YNASQLITQR ESVSREIRKI LTLRAANFHI ALDDVSITGL
TFGKEFTAAI EGKQVAAQEA ERAKFIVEKA EQDKRSAVIR AEGEAKSAQL IGQAIANNQA
FLTLRKIEAA REIAQTISRS ANKVYLSSND LLLNLQAMDL DVKPKK
