AST8_DROME
ID AST8_DROME Reviewed; 486 AA.
AC P09775; Q541C1; Q9W5G2;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1990, sequence version 2.
DT 03-AUG-2022, entry version 187.
DE RecName: Full=Achaete-scute complex protein T8;
DE AltName: Full=Protein asense;
GN Name=ase; Synonyms=T8; ORFNames=CG3258;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=Canton-S;
RX PubMed=2510998; DOI=10.1002/j.1460-2075.1989.tb08527.x;
RA Gonzalez F., Romani S., Cubas P., Modolell J., Campuzano S.;
RT "Molecular analysis of the asense gene, a member of the achaete-scute
RT complex of Drosophila melanogaster, and its novel role in optic lobe
RT development.";
RL EMBO J. 8:3553-3562(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 16-486.
RC STRAIN=Canton-S;
RX PubMed=2461300; DOI=10.1002/j.1460-2075.1988.tb03108.x;
RA Alonso M.C., Cabrera C.V.;
RT "The achaete-scute gene complex of Drosophila melanogaster comprises four
RT homologous genes.";
RL EMBO J. 7:2585-2591(1988).
CC -!- FUNCTION: Involved in the determination of the neuronal precursors of
CC optic lobes in the central nervous system.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein.
CC -!- TISSUE SPECIFICITY: L(1)SC, SC and AC strongly label the presumptive
CC stomatogastric nervous system, while ASE is more prominent in the
CC presumptive procephalic lobe.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA31066.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X52892; CAA37074.1; -; Genomic_DNA.
DR EMBL; AE014298; AAF45502.1; -; Genomic_DNA.
DR EMBL; AL009188; CAA15673.1; -; Genomic_DNA.
DR EMBL; AY095069; AAM11397.1; -; mRNA.
DR EMBL; X12550; CAA31066.1; ALT_FRAME; Genomic_DNA.
DR PIR; S06949; S06949.
DR RefSeq; NP_476694.1; NM_057346.5.
DR AlphaFoldDB; P09775; -.
DR SMR; P09775; -.
DR BioGRID; 57558; 15.
DR DIP; DIP-636N; -.
DR IntAct; P09775; 11.
DR STRING; 7227.FBpp0070074; -.
DR PaxDb; P09775; -.
DR PRIDE; P09775; -.
DR DNASU; 30985; -.
DR EnsemblMetazoa; FBtr0070075; FBpp0070074; FBgn0000137.
DR GeneID; 30985; -.
DR KEGG; dme:Dmel_CG3258; -.
DR CTD; 30985; -.
DR FlyBase; FBgn0000137; ase.
DR VEuPathDB; VectorBase:FBgn0000137; -.
DR eggNOG; KOG4029; Eukaryota.
DR GeneTree; ENSGT00940000172534; -.
DR HOGENOM; CLU_034324_0_0_1; -.
DR InParanoid; P09775; -.
DR OMA; ILPAFYD; -.
DR OrthoDB; 1131543at2759; -.
DR PhylomeDB; P09775; -.
DR SignaLink; P09775; -.
DR BioGRID-ORCS; 30985; 0 hits in 3 CRISPR screens.
DR ChiTaRS; tsr; fly.
DR GenomeRNAi; 30985; -.
DR PRO; PR:P09775; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0000137; Expressed in somatic precursor cell (Drosophila) and 56 other tissues.
DR Genevisible; P09775; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:FlyBase.
DR GO; GO:0042803; F:protein homodimerization activity; IPI:FlyBase.
DR GO; GO:0007417; P:central nervous system development; NAS:FlyBase.
DR GO; GO:0022416; P:chaeta development; IMP:FlyBase.
DR GO; GO:0008407; P:chaeta morphogenesis; IMP:FlyBase.
DR GO; GO:0061331; P:epithelial cell proliferation involved in Malpighian tubule morphogenesis; IMP:FlyBase.
DR GO; GO:0061382; P:Malpighian tubule tip cell differentiation; IMP:FlyBase.
DR GO; GO:0045839; P:negative regulation of mitotic nuclear division; IMP:FlyBase.
DR GO; GO:0007399; P:nervous system development; IGI:FlyBase.
DR GO; GO:0007400; P:neuroblast fate determination; IMP:FlyBase.
DR GO; GO:0007422; P:peripheral nervous system development; NAS:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:FlyBase.
DR GO; GO:2000177; P:regulation of neural precursor cell proliferation; IMP:FlyBase.
DR GO; GO:0050767; P:regulation of neurogenesis; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR InterPro; IPR015660; MASH1/Ascl1a-like.
DR PANTHER; PTHR13935; PTHR13935; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Neurogenesis;
KW Reference proteome.
FT CHAIN 1..486
FT /note="Achaete-scute complex protein T8"
FT /id="PRO_0000127138"
FT DOMAIN 159..223
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 75..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..115
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 486 AA; 53223 MW; 17D5A1A0B9A5B222 CRC64;
MAALSFSPSP PPKENPKENP NPGIKTTLKP FGKITVHNVL SESGANALQQ HIANQNTIIR
KIRDFGMLGA VQSAAASTTN TTPISSQRKR PLGESQKQNR HNQQNQQLSK TSVPAKKCKT
NKKLAVERPP KAGTISHPHK SQSDQSFGTP GRKGLPLPQA VARRNARERN RVKQVNNGFA
LLREKIPEEV SEAFEAQGAG RGASKKLSKV ETLRMAVEYI RSLEKLLGFD FPPLNSQGNS
SGSGDDSFMF IKDEFDCLDE HFDDSLSNYE MDEQQTVQQT LSEDMLNPPQ ASDLLPSLTT
LNGLQYIRIP GTNTYQLLTT DLLGDLSHEQ KLEETAASGQ LSRSPVPQKV VRSPCSSPVS
PVASTELLLQ TQTCATPLQQ QVIKQEYVST NISSSSNAQT SPQQQQQVQN LGSSPILPAF
YDQEPVSFYD NVVLPGFKKE FSDILQQDQP NNTTAGCLSD ESMIDAIDWW EAHAPKSNGA
CTNLSV
