PHC2_DANRE
ID PHC2_DANRE Reviewed; 827 AA.
AC Q8QHL5; Q6DGB9; Q6TGW0; Q7ZYX8; Q8QHL4;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=Polyhomeotic-like protein 2;
GN Name=phc2; Synonyms=edr2, ph2;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RX PubMed=11846397; DOI=10.1006/bbrc.2002.6447;
RA Kawamura A., Yamada K., Fujimori K., Higashinakagawa T.;
RT "Alternative transcripts of a polyhomeotic gene homolog are expressed in
RT distinct regions of somites during segmentation of zebrafish embryos.";
RL Biochem. Biophys. Res. Commun. 291:245-254(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Kidney marrow;
RX PubMed=15520368; DOI=10.1073/pnas.0407241101;
RA Song H.-D., Sun X.-J., Deng M., Zhang G.-W., Zhou Y., Wu X.-Y., Sheng Y.,
RA Chen Y., Ruan Z., Jiang C.-L., Fan H.-Y., Zon L.I., Kanki J.P., Liu T.X.,
RA Look A.T., Chen Z.;
RT "Hematopoietic gene expression profile in zebrafish kidney marrow.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:16240-16245(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=AB; TISSUE=Embryo;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of a Polycomb group (PcG) multiprotein PRC1-like
CC complex, a complex class required to maintain the transcriptionally
CC repressive state of many genes, including Hox genes, throughout
CC development. PcG PRC1 complex acts via chromatin remodeling and
CC modification of histones; it mediates monoubiquitination of histone H2A
CC 'Lys-119', rendering chromatin heritably changed in its expressibility
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of a PRC1-like complex.
CC {ECO:0000250|UniProtKB:Q8IXK0}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8IXK0}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=ph2-alpha;
CC IsoId=Q8QHL5-1; Sequence=Displayed;
CC Name=2; Synonyms=ph2-beta;
CC IsoId=Q8QHL5-2; Sequence=VSP_016920;
CC -!- TISSUE SPECIFICITY: Isoform 1 expression is stronger at the posterior
CC border than in the anterior region within individual somites; On the
CC contrary, isoform 2 expression is higher at the posterior border.
CC -!- DEVELOPMENTAL STAGE: Isoform 1 is expressed during the segmentation
CC period and is initially detected at the 7-8 somite stage; Isoform 2 is
CC expressed at all developmental stages and appears in the first somites.
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DR EMBL; AB064940; BAB83527.1; -; mRNA.
DR EMBL; AB064941; BAB83528.1; -; mRNA.
DR EMBL; AY398396; AAQ97829.1; -; mRNA.
DR EMBL; BC044345; AAH44345.1; -; mRNA.
DR EMBL; BC065351; AAH65351.1; -; mRNA.
DR EMBL; BC076431; AAH76431.1; -; mRNA.
DR RefSeq; NP_001129960.1; NM_001136488.2. [Q8QHL5-2]
DR AlphaFoldDB; Q8QHL5; -.
DR SMR; Q8QHL5; -.
DR BioGRID; 79680; 4.
DR STRING; 7955.ENSDARP00000117469; -.
DR PaxDb; Q8QHL5; -.
DR Ensembl; ENSDART00000054362; ENSDARP00000054361; ENSDARG00000056695. [Q8QHL5-2]
DR GeneID; 171465; -.
DR KEGG; dre:171465; -.
DR CTD; 171465; -.
DR ZFIN; ZDB-GENE-020312-1; phc2a.
DR eggNOG; ENOG502QS5Q; Eukaryota.
DR GeneTree; ENSGT00940000154964; -.
DR HOGENOM; CLU_047131_0_0_1; -.
DR InParanoid; Q8QHL5; -.
DR OMA; RPTLPHI; -.
DR PhylomeDB; Q8QHL5; -.
DR TreeFam; TF331299; -.
DR PRO; PR:Q8QHL5; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 23.
DR Bgee; ENSDARG00000056695; Expressed in presomitic mesoderm and 29 other tissues.
DR ExpressionAtlas; Q8QHL5; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0031519; C:PcG protein complex; ISS:UniProtKB.
DR GO; GO:0035102; C:PRC1 complex; ISS:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IBA:GO_Central.
DR Gene3D; 1.10.150.50; -; 1.
DR Gene3D; 3.30.60.160; -; 1.
DR InterPro; IPR001660; SAM.
DR InterPro; IPR013761; SAM/pointed_sf.
DR InterPro; IPR012313; Znf_FCS.
DR InterPro; IPR038603; Znf_FCS_sf.
DR Pfam; PF00536; SAM_1; 1.
DR SMART; SM00454; SAM; 1.
DR SUPFAM; SSF47769; SSF47769; 1.
DR PROSITE; PS50105; SAM_DOMAIN; 1.
DR PROSITE; PS51024; ZF_FCS; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Metal-binding;
KW Nucleus; Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..827
FT /note="Polyhomeotic-like protein 2"
FT /id="PRO_0000076288"
FT DOMAIN 763..827
FT /note="SAM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00184"
FT ZN_FING 609..643
FT /note="FCS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00367"
FT REGION 1..78
FT /note="Disordered"
FT /evidence="ECO:0000250|UniProtKB:Q9QWH1"
FT REGION 282..316
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 482..545
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 653..730
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 540..570
FT /note="HD1"
FT COMPBIAS 7..40
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..316
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 517..539
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 662..680
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 681..730
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 618
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00367"
FT BINDING 621
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00367"
FT BINDING 637
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00367"
FT BINDING 641
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00367"
FT VAR_SEQ 1..517
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11846397,
FT ECO:0000303|PubMed:15520368, ECO:0000303|Ref.3"
FT /id="VSP_016920"
FT CONFLICT 698
FT /note="L -> F (in Ref. 3; AAH76431)"
FT /evidence="ECO:0000305"
FT CONFLICT 705..706
FT /note="Missing (in Ref. 3; AAH76431)"
FT /evidence="ECO:0000305"
FT CONFLICT 724
FT /note="S -> T (in Ref. 3; AAH44345)"
FT /evidence="ECO:0000305"
FT CONFLICT 740
FT /note="S -> K (in Ref. 2; AAQ97829)"
FT /evidence="ECO:0000305"
FT CONFLICT 764
FT /note="N -> K (in Ref. 2; AAQ97829 and 3; AAH76431)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 827 AA; 88680 MW; F623EC8F0F95FE4A CRC64;
MEKEQEQGSV ASSASVTIPS TTSVSTSTSA GTLSNSSSRQ QTVPQISVYG GITDRQTVQV
IQQALNRQPS TVAAQYLQQM YAAQQQHLML QTAALQQQHL SLAAVQQASI VAGRQSCSQH
GSTSQQTVAS QTTINLATSP AARHLISRAQ SGSSVPVCIG QQAVLLGNSS TPTLTASQAQ
MYLRAQMAQQ TNLVQVARSL GRAVPLSSQL IFTPTASVTA IQPEAPAPSI NTPPTSGQVQ
NLALRSQQGA LTSTLSQSQL QSLSVKQSVA SVTGQSVARL KGLGAEPSPQ GTAAKASPAE
TSSETTAKND KTSDLTTSVC PSVTSVAGHP FISTAYTQIQ THQLLQQHKQ QFVIQQQPQI
LQRGQAQLLE AAAIHPHTVQ AVAIQSALPA QPQQCPIPLL PKVPVTCQQA TIFHSTTVSQ
QALAQNGQAH ILTHTKAPPL QLTAVKCQIH PVQIQQGVAS QNVPDKDTPV LLEPQLLSIP
IQEPTRTELR QSDKESQVKE NTQGSVNVKA GVGSPPAMTS GSGNNAPTVT GSAPQNGESK
PPPQAVVKPQ ILTHVIEGFV IQEGAEPFPV ERPSLLIENL KQKQHHAYSD LQKHNADSEM
EDLSLQELNN QPEPVRTCEF CGNVDFAFNF KRSKRFCSTV CAKRYNVGCT KRMGLFPGKS
SPEDTKKPKA SDESPKNCST ETRKRNPSIQ TTTGASLLSP HPSHPSHGES SQCSDMSSYE
EPISPLSNSS FGAPIEHEES FDHSRELTPL LTQHFLASDP TKWNVEDVYE FICSLPGCHE
IAEEFRSQEI DGQALMLLKE DHLMSTMNIK LGPALKIFAR ISMLKDS
