PHCA_RALSO
ID PHCA_RALSO Reviewed; 347 AA.
AC P52698; Q9AIT9; Q9RBU2;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 25-MAY-2022, entry version 128.
DE RecName: Full=HTH-type transcriptional regulator PhcA;
DE AltName: Full=Virulence genes transcriptional regulator PhcA;
GN Name=phcA; OrderedLocusNames=RSc2748; ORFNames=RS00106;
OS Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=267608;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AW1;
RX PubMed=8366033; DOI=10.1128/jb.175.17.5477-5487.1993;
RA Brumbley S.M., Carney B.F., Denny T.P.;
RT "Phenotype conversion in Pseudomonas solanacearum due to spontaneous
RT inactivation of PhcA, a putative LysR transcriptional regulator.";
RL J. Bacteriol. 175:5477-5487(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ACH0158 / Biovar 2;
RX PubMed=10913109; DOI=10.1128/jb.182.16.4673-4676.2000;
RA Jeong E.-L., Timmis J.N.;
RT "Novel insertion sequence elements associated with genetic heterogeneity
RT and phenotype conversion in Ralstonia solanacearum.";
RL J. Bacteriol. 182:4673-4676(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Thoquet P., Trigalet-Demery D., Trigalet A.;
RT "Plant-induced phase reversion from non-pathogenic to pathogenic form of
RT Ralstonia solanacearum.";
RL Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GMI1000;
RX PubMed=11823852; DOI=10.1038/415497a;
RA Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL Nature 415:497-502(2002).
CC -!- FUNCTION: Regulates the transcription of one or more of the genes
CC involved in virulence.
CC -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC {ECO:0000305}.
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DR EMBL; L19269; AAC36989.1; -; Unassigned_DNA.
DR EMBL; AF184046; AAD56543.1; -; Genomic_DNA.
DR EMBL; AF239238; AAK15051.1; -; Genomic_DNA.
DR EMBL; AL646052; CAD16455.1; -; Genomic_DNA.
DR PIR; A48655; A48655.
DR RefSeq; WP_011002655.1; NC_003295.1.
DR AlphaFoldDB; P52698; -.
DR SMR; P52698; -.
DR STRING; 267608.RSc2748; -.
DR EnsemblBacteria; CAD16455; CAD16455; RSc2748.
DR GeneID; 60502250; -.
DR KEGG; rso:RSc2748; -.
DR PATRIC; fig|267608.8.peg.2795; -.
DR eggNOG; COG0583; Bacteria.
DR HOGENOM; CLU_039613_39_0_4; -.
DR OMA; MTWNESG; -.
DR PHI-base; PHI:7611; -.
DR Proteomes; UP000001436; Chromosome.
DR GO; GO:0032993; C:protein-DNA complex; IMP:CollecTF.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IMP:CollecTF.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IMP:CollecTF.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR005119; LysR_subst-bd.
DR InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00126; HTH_1; 1.
DR Pfam; PF03466; LysR_substrate; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50931; HTH_LYSR; 1.
PE 3: Inferred from homology;
KW DNA-binding; Reference proteome; Transcription; Transcription regulation;
KW Virulence.
FT CHAIN 1..347
FT /note="HTH-type transcriptional regulator PhcA"
FT /id="PRO_0000105744"
FT DOMAIN 1..61
FT /note="HTH lysR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT DNA_BIND 21..40
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT REGION 316..347
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 329..347
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 92
FT /note="L -> A (in strain: AW1 and ACH0158)"
FT VARIANT 100
FT /note="I -> V (in strain: AW1 and ACH0158)"
FT VARIANT 185
FT /note="H -> G (in strain: ACH0158)"
FT VARIANT 185
FT /note="H -> R (in strain: AW1)"
FT VARIANT 206
FT /note="T -> S (in strain: AW1 and ACH0158)"
FT VARIANT 247
FT /note="M -> L (in strain: AW1 and ACH0158)"
FT VARIANT 262
FT /note="T -> A (in strain: AW1 and ACH0158)"
FT VARIANT 264
FT /note="N -> S (in strain: ACH0158)"
FT VARIANT 314
FT /note="I -> N (in strain: AW1 and ACH0158)"
FT VARIANT 329
FT /note="S -> A (in strain: AW1 and ACH0158)"
FT VARIANT 347
FT /note="V -> A (in strain: AW1 and ACH0158)"
SQ SEQUENCE 347 AA; 38791 MW; 00DD51779F21782B CRC64;
MVNVDTKLLV IFVELLSKRN ATYVAEKMHM TAPAVSHSLG RLREIFDDPL FIRVPHGLTP
TPKALELGPK VREMLDLWAA INEGDIATFD PLEAAGTFNI SFAGTLGDAL FDRFLLRVKR
LAPGLQVRLT ESSSWEADVA AMRSNELDLA FSPFPTRHPE IVEEVVTSFN MWVCARKDHP
VLKDHCSLDQ YLECEHIFIA QGNPGTRAAP SLIPLDYALQ QRGLKRHSTM TVHAWRTQAE
VAAQTDMIFT VNSLMKDLVC ETYNLNAFPL PSELETVLGL NMLWHRSRNT HPMLVWARNL
FKQVVAEYTG KASIAPMHPP MLTDDSGKSG KTGKGDAEKE DESRLSV
