PHCA_SYNY3
ID PHCA_SYNY3 Reviewed; 162 AA.
AC Q54715;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=C-phycocyanin alpha subunit;
GN Name=cpcA; OrderedLocusNames=sll1578;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Plank T., Anderson L.K.;
RT "Cloning and sequence analysis of the genes encoding CpcB and CpcA from
RT Synechocystis sp. PCC 6803.";
RL (er) Plant Gene Register PGR95-087(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
RN [3]
RP PROTEIN SEQUENCE OF 1-19.
RX PubMed=9298645; DOI=10.1002/elps.1150180806;
RA Sazuka T., Ohara O.;
RT "Towards a proteome project of cyanobacterium Synechocystis sp. strain
RT PCC6803: linking 130 protein spots with their respective genes.";
RL Electrophoresis 18:1252-1258(1997).
RN [4]
RP SUBUNIT.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=31015331; DOI=10.1128/mbio.00669-19;
RA Liu H., Weisz D.A., Zhang M.M., Cheng M., Zhang B., Zhang H.,
RA Gerstenecker G.S., Pakrasi H.B., Gross M.L., Blankenship R.E.;
RT "Phycobilisomes Harbor FNRL in Cyanobacteria.";
RL MBio 10:0-0(2019).
RN [5] {ECO:0007744|PDB:4F0T}
RP X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) IN COMPLEX WITH PHYCOCYANOBILIN
RP CHROMOPHORE, AND SUBUNIT.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=23201474; DOI=10.1016/j.bbabio.2012.11.006;
RA Marx A., Adir N.;
RT "Allophycocyanin and phycocyanin crystal structures reveal facets of
RT phycobilisome assembly.";
RL Biochim. Biophys. Acta 1827:311-318(2013).
CC -!- FUNCTION: Light-harvesting photosynthetic bile pigment-protein from the
CC phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major
CC phycobiliprotein in the PBS rod.
CC -!- SUBUNIT: Heterodimer of an alpha and a beta chain, which further
CC assembles into trimers. The trimers assemble into hexamers, although
CC these were not seen in the crystallographic studies (PubMed:23201474).
CC Part of 2 PBS rod complexes, the conventional CpcG-PBS rod and a
CC photosystem I-specific CpcL-PBS rod, both of which include ferredoxin--
CC NADP reductase (petH) (PubMed:31015331). {ECO:0000269|PubMed:23201474,
CC ECO:0000269|PubMed:31015331}.
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}; Cytoplasmic side
CC {ECO:0000250}. Note=Part of the phycobilisome rod. {ECO:0000250,
CC ECO:0000269|PubMed:31015331}.
CC -!- PTM: Contains one covalently linked phycocyanobilin chromophore.
CC {ECO:0000269|PubMed:23201474}.
CC -!- SIMILARITY: Belongs to the phycobiliprotein family. {ECO:0000305}.
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DR EMBL; U34930; AAA91033.1; -; Genomic_DNA.
DR EMBL; BA000022; BAA17231.1; -; Genomic_DNA.
DR PIR; S75317; S75317.
DR PDB; 4F0T; X-ray; 2.25 A; A=1-162.
DR PDBsum; 4F0T; -.
DR AlphaFoldDB; Q54715; -.
DR SMR; Q54715; -.
DR IntAct; Q54715; 7.
DR STRING; 1148.1652308; -.
DR PaxDb; Q54715; -.
DR PRIDE; Q54715; -.
DR EnsemblBacteria; BAA17231; BAA17231; BAA17231.
DR KEGG; syn:sll1578; -.
DR eggNOG; ENOG502Z85C; Bacteria.
DR InParanoid; Q54715; -.
DR OMA; TTQMQGP; -.
DR PhylomeDB; Q54715; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0030089; C:phycobilisome; IDA:UniProtKB.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.490.20; -; 1.
DR InterPro; IPR009050; Globin-like_sf.
DR InterPro; IPR012128; Phycobilisome_asu/bsu.
DR InterPro; IPR038719; Phycobilisome_asu/bsu_sf.
DR InterPro; IPR006246; Phycocyanin_a.
DR Pfam; PF00502; Phycobilisome; 1.
DR PIRSF; PIRSF000081; Phycocyanin; 1.
DR SUPFAM; SSF46458; SSF46458; 1.
DR TIGRFAMs; TIGR01338; phycocy_alpha; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antenna complex; Bile pigment; Chromophore;
KW Direct protein sequencing; Electron transport; Membrane; Photosynthesis;
KW Phycobilisome; Reference proteome; Thylakoid; Transport.
FT CHAIN 1..162
FT /note="C-phycocyanin alpha subunit"
FT /id="PRO_0000199133"
FT BINDING 84
FT /ligand="(2R,3E)-phycocyanobilin"
FT /ligand_id="ChEBI:CHEBI:85275"
FT /note="covalent, via 1 link"
FT /evidence="ECO:0000269|PubMed:23201474,
FT ECO:0007744|PDB:4F0T"
FT HELIX 4..14
FT /evidence="ECO:0007829|PDB:4F0T"
FT HELIX 21..46
FT /evidence="ECO:0007829|PDB:4F0T"
FT HELIX 48..62
FT /evidence="ECO:0007829|PDB:4F0T"
FT HELIX 64..67
FT /evidence="ECO:0007829|PDB:4F0T"
FT HELIX 78..101
FT /evidence="ECO:0007829|PDB:4F0T"
FT HELIX 105..110
FT /evidence="ECO:0007829|PDB:4F0T"
FT TURN 111..114
FT /evidence="ECO:0007829|PDB:4F0T"
FT HELIX 115..121
FT /evidence="ECO:0007829|PDB:4F0T"
FT HELIX 126..137
FT /evidence="ECO:0007829|PDB:4F0T"
FT HELIX 144..161
FT /evidence="ECO:0007829|PDB:4F0T"
SQ SEQUENCE 162 AA; 17587 MW; 59F9935B6BB6773A CRC64;
MKTPLTEAVS TADSQGRFLS STELQIAFGR LRQANAGLQA AKALTDNAQS LVNGAAQAVY
NKFPYTTQTQ GNNFAADQRG KDKCARDIGY YLRIVTYCLV AGGTGPLDEY LIAGIDEINR
TFDLSPSWYV EALKYIKANH GLSGDARDEA NSYLDYAINA LS
